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Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli

Ward De Spiegelaere (UGent) , Pieter Cornillie (UGent) , Tim Erkens (UGent) , Denis Van Loo (UGent) , Christophe Casteleyn (UGent) , Mario Van Poucke (UGent) , Christian Burvenich (UGent) , Luc Van Hoorebeke (UGent) , Chris Van Ginneken, Luc Peelman (UGent) , et al.
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Abstract
The development and growth of renal glomeruli is regulated by specific angiogenic growth factors, including vascular endothelial growth factor (VEGF) and the angiopoietins (ANGPT1 and ANGPT2). The expression of these factors has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros, which can function as an interesting model for glomerular development and senescence. In this study, the presence of the angiogenic growth factors was studied in developing porcine mesonephroi, using IHC and real-time RT-qPCR on laser capture microdissected glomeruli. In addition, mesonephric glomerular growth was measured by using stereological methods. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. The VEGF receptor VEGFR1 was stably expressed during the whole lifespan of mesonephric glomeruli, whereas VEGFR2 mRNA was only upregulated in early glomerulogenesis, suggesting that VEGFR2 is important for the vascular growth but that VEGFR1 is important for the maintenance of endothelial fenestrations. (J Histochem Cytochem 58:1045-1056, 2010)
Keywords
real-time PCR, 3D reconstruction, TEK, FLT1, RECEPTORS, MECHANISMS, CELLS, VEGF, KIDNEY DEVELOPMENT, VASCULAR MORPHOGENESIS, MICROVASCULAR GROWTH, IN-VIVO, KDR, fenestrations, TIE-2 LIGAND ANGIOPOIETIN-2, micro-CT, INTUSSUSCEPTIVE CAPILLARY GROWTH, mesonephros, kidney, intussusceptive angiogenesis

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Chicago
De Spiegelaere, Ward, Pieter Cornillie, Tim Erkens, Denis Van Loo, Christophe Casteleyn, Mario Van Poucke, Christian Burvenich, et al. 2010. “Expression and Localization of Angiogenic Growth Factors in Developing Porcine Mesonephric Glomeruli.” Journal of Histochemistry & Cytochemistry 58 (12): 1045–1056.
APA
De Spiegelaere, W., Cornillie, P., Erkens, T., Van Loo, D., Casteleyn, C., Van Poucke, M., Burvenich, C., et al. (2010). Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 58(12), 1045–1056.
Vancouver
1.
De Spiegelaere W, Cornillie P, Erkens T, Van Loo D, Casteleyn C, Van Poucke M, et al. Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 2010;58(12):1045–56.
MLA
De Spiegelaere, Ward, Pieter Cornillie, Tim Erkens, et al. “Expression and Localization of Angiogenic Growth Factors in Developing Porcine Mesonephric Glomeruli.” JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 58.12 (2010): 1045–1056. Print.
@article{1163722,
  abstract     = {The development and growth of renal glomeruli is regulated by specific angiogenic growth factors, including vascular endothelial growth factor (VEGF) and the angiopoietins (ANGPT1 and ANGPT2). The expression of these factors has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros, which can function as an interesting model for glomerular development and senescence. In this study, the presence of the angiogenic growth factors was studied in developing porcine mesonephroi, using IHC and real-time RT-qPCR on laser capture microdissected glomeruli. In addition, mesonephric glomerular growth was measured by using stereological methods. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. The VEGF receptor VEGFR1 was stably expressed during the whole lifespan of mesonephric glomeruli, whereas VEGFR2 mRNA was only upregulated in early glomerulogenesis, suggesting that VEGFR2 is important for the vascular growth but that VEGFR1 is important for the maintenance of endothelial fenestrations. (J Histochem Cytochem 58:1045-1056, 2010)},
  author       = {De Spiegelaere, Ward and Cornillie, Pieter and Erkens, Tim and Van Loo, Denis and Casteleyn, Christophe and Van Poucke, Mario and Burvenich, Christian and Van Hoorebeke, Luc and Van Ginneken, Chris and Peelman, Luc and Van Den Broeck, Wim},
  issn         = {0022-1554},
  journal      = {JOURNAL OF HISTOCHEMISTRY \& CYTOCHEMISTRY},
  language     = {eng},
  number       = {12},
  pages        = {1045--1056},
  title        = {Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli},
  url          = {http://dx.doi.org/10.1369/jhc.2010.956557},
  volume       = {58},
  year         = {2010},
}

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