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Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli

Ward De Spiegelaere UGent, Pieter Cornillie UGent, Tim Erkens UGent, Denis Van Loo UGent, Christophe Casteleyn UGent, Mario Van Poucke UGent, Christian Burvenich UGent, Luc Van Hoorebeke UGent, Chris Van Ginneken and Luc Peelman UGent, et al. (2010) JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 58(12). p.1045-1056
abstract
The development and growth of renal glomeruli is regulated by specific angiogenic growth factors, including vascular endothelial growth factor (VEGF) and the angiopoietins (ANGPT1 and ANGPT2). The expression of these factors has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros, which can function as an interesting model for glomerular development and senescence. In this study, the presence of the angiogenic growth factors was studied in developing porcine mesonephroi, using IHC and real-time RT-qPCR on laser capture microdissected glomeruli. In addition, mesonephric glomerular growth was measured by using stereological methods. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. The VEGF receptor VEGFR1 was stably expressed during the whole lifespan of mesonephric glomeruli, whereas VEGFR2 mRNA was only upregulated in early glomerulogenesis, suggesting that VEGFR2 is important for the vascular growth but that VEGFR1 is important for the maintenance of endothelial fenestrations. (J Histochem Cytochem 58:1045-1056, 2010)
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
real-time PCR, 3D reconstruction, TEK, FLT1, RECEPTORS, MECHANISMS, CELLS, VEGF, KIDNEY DEVELOPMENT, VASCULAR MORPHOGENESIS, MICROVASCULAR GROWTH, IN-VIVO, KDR, fenestrations, TIE-2 LIGAND ANGIOPOIETIN-2, micro-CT, INTUSSUSCEPTIVE CAPILLARY GROWTH, mesonephros, kidney, intussusceptive angiogenesis
journal title
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
J. Histochem. Cytochem.
volume
58
issue
12
pages
1045 - 1056
Web of Science type
Article
Web of Science id
000284449300001
JCR category
CELL BIOLOGY
JCR impact factor
2.381 (2010)
JCR rank
116/174 (2010)
JCR quartile
3 (2010)
ISSN
0022-1554
DOI
10.1369/jhc.2010.956557
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1163722
handle
http://hdl.handle.net/1854/LU-1163722
date created
2011-02-22 10:47:47
date last changed
2011-02-22 11:55:36
@article{1163722,
  abstract     = {The development and growth of renal glomeruli is regulated by specific angiogenic growth factors, including vascular endothelial growth factor (VEGF) and the angiopoietins (ANGPT1 and ANGPT2). The expression of these factors has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros, which can function as an interesting model for glomerular development and senescence. In this study, the presence of the angiogenic growth factors was studied in developing porcine mesonephroi, using IHC and real-time RT-qPCR on laser capture microdissected glomeruli. In addition, mesonephric glomerular growth was measured by using stereological methods. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. The VEGF receptor VEGFR1 was stably expressed during the whole lifespan of mesonephric glomeruli, whereas VEGFR2 mRNA was only upregulated in early glomerulogenesis, suggesting that VEGFR2 is important for the vascular growth but that VEGFR1 is important for the maintenance of endothelial fenestrations. (J Histochem Cytochem 58:1045-1056, 2010)},
  author       = {De Spiegelaere, Ward and Cornillie, Pieter and Erkens, Tim and Van Loo, Denis and Casteleyn, Christophe and Van Poucke, Mario and Burvenich, Christian and Van Hoorebeke, Luc and Van Ginneken, Chris and Peelman, Luc and Van Den Broeck, Wim},
  issn         = {0022-1554},
  journal      = {JOURNAL OF HISTOCHEMISTRY \& CYTOCHEMISTRY},
  keyword      = {real-time PCR,3D reconstruction,TEK,FLT1,RECEPTORS,MECHANISMS,CELLS,VEGF,KIDNEY DEVELOPMENT,VASCULAR MORPHOGENESIS,MICROVASCULAR GROWTH,IN-VIVO,KDR,fenestrations,TIE-2 LIGAND ANGIOPOIETIN-2,micro-CT,INTUSSUSCEPTIVE CAPILLARY GROWTH,mesonephros,kidney,intussusceptive angiogenesis},
  language     = {eng},
  number       = {12},
  pages        = {1045--1056},
  title        = {Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli},
  url          = {http://dx.doi.org/10.1369/jhc.2010.956557},
  volume       = {58},
  year         = {2010},
}

Chicago
DE SPIEGELAERE, WARD, Pieter Cornillie, Tim Erkens, Denis Van Loo, Christophe Casteleyn, Mario Van Poucke, Christian Burvenich, et al. 2010. “Expression and Localization of Angiogenic Growth Factors in Developing Porcine Mesonephric Glomeruli.” Journal of Histochemistry & Cytochemistry 58 (12): 1045–1056.
APA
DE SPIEGELAERE, W., Cornillie, P., Erkens, T., Van Loo, D., Casteleyn, C., Van Poucke, M., Burvenich, C., et al. (2010). Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 58(12), 1045–1056.
Vancouver
1.
DE SPIEGELAERE W, Cornillie P, Erkens T, Van Loo D, Casteleyn C, Van Poucke M, et al. Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 2010;58(12):1045–56.
MLA
DE SPIEGELAERE, WARD, Pieter Cornillie, Tim Erkens, et al. “Expression and Localization of Angiogenic Growth Factors in Developing Porcine Mesonephric Glomeruli.” JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 58.12 (2010): 1045–1056. Print.