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Investigation of the potential association of vitamin D binding protein with lipoproteins

Marijn Speeckaert UGent, Youri Taes, Marc De Buyzere, Armand Christophe, Jean Kaufman UGent and Joris Delanghe UGent (2010) ANNALS OF CLINICAL BIOCHEMISTRY. 47(2). p.143-150
abstract
BACKGROUND: Vitamin D binding protein (DBP) acts as a vitamin D carrier and an actin scavenger. We have previously reported a correlation between serum DBP and lipid parameters in a cystic fibrosis population. In the present study, the relationship between serum DBP, lipoprotein fractions and vitamin D is investigated. METHODS: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)- and 1.25(OH)(2)-vitamin D(3) were assayed by radioimmunoassay. Total cholesterol, high-density lipoprotein cholesterol (HDL), triglycerides and insulin were measured using routine methods. Low-density lipoprotein-cholesterol (LDL) was calculated according to Friedewald's formula. RESULTS: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D(3) in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D(3)/DBP ratio correlated negatively with serum triglyceride concentration and body mass index (BMI). The actin-bound DBP complex was identified with Western blot. CONCLUSIONS: The lipid-bound DBP fraction may be of greater importance than initially thought. The present findings may have clinical consequences in view of the important physiological role of DBP.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
POLYMORPHISM, DBP, LOW-DENSITY LIPOPROTEIN, BONE-MINERAL DENSITY, GC-GLOBULIN, CHOLESTEROL, ASSAY, PLASMA, PHENOTYPE, SERUM
journal title
ANNALS OF CLINICAL BIOCHEMISTRY
Ann. Clin. Biochem.
volume
47
issue
2
pages
143 - 150
Web of Science type
Article
Web of Science id
000288147400007
JCR category
MEDICAL LABORATORY TECHNOLOGY
JCR impact factor
2.209 (2010)
JCR rank
8/30 (2010)
JCR quartile
2 (2010)
ISSN
0004-5632
DOI
10.1258/acb.2009.009018
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1162537
handle
http://hdl.handle.net/1854/LU-1162537
date created
2011-02-21 14:32:32
date last changed
2017-05-15 13:06:56
@article{1162537,
  abstract     = {BACKGROUND: Vitamin D binding protein (DBP) acts as a vitamin D carrier and an actin scavenger. We have previously reported a correlation between serum DBP and lipid parameters in a cystic fibrosis population. In the present study, the relationship between serum DBP, lipoprotein fractions and vitamin D is investigated.
METHODS: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)- and 1.25(OH)(2)-vitamin D(3) were assayed by radioimmunoassay. Total cholesterol, high-density lipoprotein cholesterol (HDL), triglycerides and insulin were measured using routine methods. Low-density lipoprotein-cholesterol (LDL) was calculated according to Friedewald's formula.
RESULTS: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D(3) in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D(3)/DBP ratio correlated negatively with serum triglyceride concentration and body mass index (BMI). The actin-bound DBP complex was identified with Western blot.
CONCLUSIONS: The lipid-bound DBP fraction may be of greater importance than initially thought. The present findings may have clinical consequences in view of the important physiological role of DBP.},
  author       = {Speeckaert, Marijn and Taes, Youri and De Buyzere, Marc and Christophe, Armand and Kaufman, Jean and Delanghe, Joris},
  issn         = {0004-5632},
  journal      = {ANNALS OF CLINICAL BIOCHEMISTRY},
  keyword      = {POLYMORPHISM,DBP,LOW-DENSITY LIPOPROTEIN,BONE-MINERAL DENSITY,GC-GLOBULIN,CHOLESTEROL,ASSAY,PLASMA,PHENOTYPE,SERUM},
  language     = {eng},
  number       = {2},
  pages        = {143--150},
  title        = {Investigation of the potential association of vitamin D binding protein with lipoproteins},
  url          = {http://dx.doi.org/10.1258/acb.2009.009018},
  volume       = {47},
  year         = {2010},
}

Chicago
Speeckaert, Marijn, Youri Taes, MARC DE BUYZERE, Armand Christophe, Jean Kaufman, and Joris Delanghe. 2010. “Investigation of the Potential Association of Vitamin D Binding Protein with Lipoproteins.” Annals of Clinical Biochemistry 47 (2): 143–150.
APA
Speeckaert, M., Taes, Y., DE BUYZERE, M., Christophe, A., Kaufman, J., & Delanghe, J. (2010). Investigation of the potential association of vitamin D binding protein with lipoproteins. ANNALS OF CLINICAL BIOCHEMISTRY, 47(2), 143–150.
Vancouver
1.
Speeckaert M, Taes Y, DE BUYZERE M, Christophe A, Kaufman J, Delanghe J. Investigation of the potential association of vitamin D binding protein with lipoproteins. ANNALS OF CLINICAL BIOCHEMISTRY. 2010;47(2):143–50.
MLA
Speeckaert, Marijn, Youri Taes, MARC DE BUYZERE, et al. “Investigation of the Potential Association of Vitamin D Binding Protein with Lipoproteins.” ANNALS OF CLINICAL BIOCHEMISTRY 47.2 (2010): 143–150. Print.