Ghent University Academic Bibliography

Advanced

In vitro culture of chestnut (Castanea sativa Mill.) using temporary immersion bioreactors

Veronique Troch UGent, Helena Sapeta, Stefaan Werbrouck UGent, Danny Geelen UGent and Marie-Christine Van Labeke UGent (2010) Acta Horticulturae. 885. p.383-389
abstract
Chestnut (Castanea sativa Mill.) is difficult to propagate by grafting and cuttings. However, as an alternative to conventional vegetative propagation methods, efforts have been made to establish reliable in vitro regeneration systems that allow clonal propagation. The two major systems are based on embryogenesis and on micropropagation of axillary shoots. The use of temporary immersion bioreactors may provide an efficient and economic tool for mass clonal propagation of chestnut. Seedlings of Castanea sativa were established in vitro and used as the starting material for the experiments. Apical and axillary explants were grown in liquid multiplication media with a composition based on the semi-solid substrates using the temporary immersion bioreactors (TIB), twin flasks system. Apical explants were qualitatively better (longer shoots, higher number of leaves) compared to axillary explants. Duration and frequency of an immersion affected the multiplication rate and quality of plantlets of Castanea sativa.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
in
Acta Horticulturae
Acta Hortic.
editor
Frantisek Sramek
volume
885
issue title
I International symposium on woody ornamentals of the temperate zone
pages
383 - 389
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
1st International symposium on Woody Ornamentals of the Temperate Zone
conference location
Pruhonice, Czech Republic
conference start
2008-05-26
conference end
2008-05-30
Web of Science type
Proceedings Paper
Web of Science id
000323620500054
ISSN
0567-7572
ISBN
9789066056633
language
English
UGent publication?
yes
classification
P1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1162275
handle
http://hdl.handle.net/1854/LU-1162275
alternative location
http://www.actahort.org/books/885/
date created
2011-02-21 13:44:00
date last changed
2017-01-02 09:53:10
@inproceedings{1162275,
  abstract     = {Chestnut (Castanea sativa Mill.) is difficult to propagate by grafting and cuttings. However, as an alternative to conventional vegetative propagation methods, efforts have been made to establish reliable in vitro regeneration systems that allow clonal propagation. The two major systems are based on embryogenesis and on micropropagation of axillary shoots. The use of temporary immersion bioreactors may provide an efficient and economic tool for mass clonal propagation of chestnut. Seedlings of Castanea sativa were established in vitro and used as the starting material for the experiments. Apical and axillary explants were grown in liquid multiplication media with a composition based on the semi-solid substrates using the temporary immersion bioreactors (TIB), twin flasks system. Apical explants were qualitatively better (longer shoots, higher number of leaves) compared to axillary explants. Duration and frequency of an immersion affected the multiplication rate and quality of plantlets of Castanea sativa.},
  author       = {Troch, Veronique and Sapeta, Helena and Werbrouck, Stefaan and Geelen, Danny and Van Labeke, Marie-Christine},
  booktitle    = {Acta Horticulturae},
  editor       = {Sramek, Frantisek},
  isbn         = {9789066056633},
  issn         = {0567-7572},
  language     = {eng},
  location     = {Pruhonice, Czech Republic},
  pages        = {383--389},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {In vitro culture of chestnut (Castanea sativa Mill.) using temporary immersion bioreactors},
  url          = {http://www.actahort.org/books/885/},
  volume       = {885},
  year         = {2010},
}

Chicago
Troch, Veronique, Helena Sapeta, Stefaan Werbrouck, Danny Geelen, and Marie-Christine Van Labeke. 2010. “In Vitro Culture of Chestnut (Castanea Sativa Mill.) Using Temporary Immersion Bioreactors.” In Acta Horticulturae, ed. Frantisek Sramek, 885:383–389. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Troch, V., Sapeta, H., Werbrouck, S., Geelen, D., & Van Labeke, M.-C. (2010). In vitro culture of chestnut (Castanea sativa Mill.) using temporary immersion bioreactors. In F. Sramek (Ed.), Acta Horticulturae (Vol. 885, pp. 383–389). Presented at the 1st International symposium on Woody Ornamentals of the Temperate Zone, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Troch V, Sapeta H, Werbrouck S, Geelen D, Van Labeke M-C. In vitro culture of chestnut (Castanea sativa Mill.) using temporary immersion bioreactors. In: Sramek F, editor. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2010. p. 383–9.
MLA
Troch, Veronique, Helena Sapeta, Stefaan Werbrouck, et al. “In Vitro Culture of Chestnut (Castanea Sativa Mill.) Using Temporary Immersion Bioreactors.” Acta Horticulturae. Ed. Frantisek Sramek. Vol. 885. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2010. 383–389. Print.