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Effect of prednisolone on cytokine synthesis in nasal polyps

C Rudack, Claus Bachert UGent and W Stoll (1999) JOURNAL OF INTERFERON AND CYTOKINE RESEARCH. 19(9). p.1031-1035
abstract
To investigate steroid effects on eosinophils and their associated cytokines in nasal polyps, we sampled nasal polyp tissue from 20 subjects during routine surgery, Freshley removed polyps were cut into small pieces and incubated in culture medium for 24 h in different concentrations of prednisolone (10(-2)-10(-6) mol/L). Cell viability was assessed by means of trypan dye exclusion of the supernatants. The number of eosinophils was quantified by means of cytocentrifuge smears stained by May-Giemsa-Grunwald stain. The cytokine protein content of interleukin-5 (IL-5), IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was measured by ELISA technique in supernatants and in homogenates of nasal polyp tissue. Our results revealed a significant reduction in the number of eosinophils and total number of vital cells at concentrations of 10(-3) and 10(-2) mol/L. GM-CSF and IL-5 protein levels were significantly reduced in supernatants and in homogenates after treatment with prednisolone, whereas IL-3 synthesis was not diminished. Corticosteroid effects known to inhibit synthesis of eosinophil-associated cytokines could be measured in terms of a significant decrease in IL-5 and GM-CSF protein level as well as in a decrease in the number of vital cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GLUCOCORTICOIDS, INHIBITION, INFLAMMATION, EOSINOPHILS, STEROIDS, TISSUE, IL-5
journal title
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
J. Interferon Cytokine Res.
volume
19
issue
9
pages
1031 - 1035
Web of Science type
Article
Web of Science id
000082641000009
ISSN
1079-9907
DOI
10.1089/107999099313253
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
116192
handle
http://hdl.handle.net/1854/LU-116192
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:24
@article{116192,
  abstract     = {To investigate steroid effects on eosinophils and their associated cytokines in nasal polyps, we sampled nasal polyp tissue from 20 subjects during routine surgery, Freshley removed polyps were cut into small pieces and incubated in culture medium for 24 h in different concentrations of prednisolone (10(-2)-10(-6) mol/L). Cell viability was assessed by means of trypan dye exclusion of the supernatants. The number of eosinophils was quantified by means of cytocentrifuge smears stained by May-Giemsa-Grunwald stain. The cytokine protein content of interleukin-5 (IL-5), IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was measured by ELISA technique in supernatants and in homogenates of nasal polyp tissue. Our results revealed a significant reduction in the number of eosinophils and total number of vital cells at concentrations of 10(-3) and 10(-2) mol/L. GM-CSF and IL-5 protein levels were significantly reduced in supernatants and in homogenates after treatment with prednisolone, whereas IL-3 synthesis was not diminished. Corticosteroid effects known to inhibit synthesis of eosinophil-associated cytokines could be measured in terms of a significant decrease in IL-5 and GM-CSF protein level as well as in a decrease in the number of vital cells.},
  author       = {Rudack, C and Bachert, Claus and Stoll, W},
  issn         = {1079-9907},
  journal      = {JOURNAL OF INTERFERON AND CYTOKINE RESEARCH},
  keyword      = {GLUCOCORTICOIDS,INHIBITION,INFLAMMATION,EOSINOPHILS,STEROIDS,TISSUE,IL-5},
  language     = {eng},
  number       = {9},
  pages        = {1031--1035},
  title        = {Effect of prednisolone on cytokine synthesis in nasal polyps},
  url          = {http://dx.doi.org/10.1089/107999099313253},
  volume       = {19},
  year         = {1999},
}

Chicago
Rudack, C, Claus Bachert, and W Stoll. 1999. “Effect of Prednisolone on Cytokine Synthesis in Nasal Polyps.” Journal of Interferon and Cytokine Research 19 (9): 1031–1035.
APA
Rudack, C., Bachert, C., & Stoll, W. (1999). Effect of prednisolone on cytokine synthesis in nasal polyps. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 19(9), 1031–1035.
Vancouver
1.
Rudack C, Bachert C, Stoll W. Effect of prednisolone on cytokine synthesis in nasal polyps. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH. 1999;19(9):1031–5.
MLA
Rudack, C, Claus Bachert, and W Stoll. “Effect of Prednisolone on Cytokine Synthesis in Nasal Polyps.” JOURNAL OF INTERFERON AND CYTOKINE RESEARCH 19.9 (1999): 1031–1035. Print.