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Intrinsics and dynamics of fat grafts: an in vitro study

Filip Stillaert, Keren M Abberton, Effie Keramidaris, Erik W Thompson, Phillip Blondeel UGent and Wayne A Morrison (2010) PLASTIC AND RECONSTRUCTIVE SURGERY. 126(4). p.1155-1162
abstract
Background: Despite a revived interest in fat grafting procedures, clinicians still fail to demonstrate clearly the in vivo behavior of fat grafts as a dynamic tissue substitute. However, the basic principles in cellular biology teach us that cells can survive and develop, provided that a structural matrix exists that directs their behavior. The purpose of this in vitro study was to analyze that behavior of crude fat grafts, cultured on a three-dimensional laminin-rich matrix. Methods: Nonprocessed, human fat biopsy specimens (approximately 1 mm) were inoculated on Matrigel-coated wells to which culture medium was added. The control group consisted of fat biopsy specimens embedded in medium alone. The cellular proliferation pattern was followed over 6 weeks. Additional cultures of primary generated cellular spheroids were performed and eventually subjected to adipogenic differentiation media. Results: A progressive outgrowth of fibroblast-like cells from the core fat biopsy specimen was observed in both groups. Within the Matrigel group, an interconnecting three-dimensional network of spindle-shaped cells was established. This new cell colony reproduced spheroids that functioned again as solitary sources of cellular proliferation. Addition of differentiation media resulted in lipid droplet deposition in the majority of generated cells, indicating the initial steps of adipogenic differentiation. Conclusions: The authors noticed that crude, nonprocessed fat biopsy specimens do have considerable potential for future tissue engineering based applications, provided that the basic principles of developmental, cellular biology are respected. Spontaneous in vitro expansion of the stromal cells present in fat grafts within autologous and injectable matrices could create "off-the-shelf" therapies for reconstructive procedures. (Plast. Reconstr. Surg. 126: 1155, 2010.)
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DIFFERENTIATION, GROWTH, STEM-CELLS, EXTRACELLULAR-MATRIX, ACID-BASED SCAFFOLDS, TISSUE, ADIPOSE PRECURSOR CELLS, IMPLANTATION, MICE
journal title
PLASTIC AND RECONSTRUCTIVE SURGERY
Plast. Reconstr. Surg.
volume
126
issue
4
pages
1155 - 1162
Web of Science type
Article
Web of Science id
000282559100080
JCR category
SURGERY
JCR impact factor
2.635 (2010)
JCR rank
38/186 (2010)
JCR quartile
1 (2010)
ISSN
0032-1052
DOI
10.1097/PRS.0b013e3181ebe224
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1156585
handle
http://hdl.handle.net/1854/LU-1156585
date created
2011-02-18 16:38:41
date last changed
2016-12-19 15:45:26
@article{1156585,
  abstract     = {Background: Despite a revived interest in fat grafting procedures, clinicians still fail to demonstrate clearly the in vivo behavior of fat grafts as a dynamic tissue substitute. However, the basic principles in cellular biology teach us that cells can survive and develop, provided that a structural matrix exists that directs their behavior. The purpose of this in vitro study was to analyze that behavior of crude fat grafts, cultured on a three-dimensional laminin-rich matrix.
Methods: Nonprocessed, human fat biopsy specimens (approximately 1 mm) were inoculated on Matrigel-coated wells to which culture medium was added. The control group consisted of fat biopsy specimens embedded in medium alone. The cellular proliferation pattern was followed over 6 weeks. Additional cultures of primary generated cellular spheroids were performed and eventually subjected to adipogenic differentiation media.
Results: A progressive outgrowth of fibroblast-like cells from the core fat biopsy specimen was observed in both groups. Within the Matrigel group, an interconnecting three-dimensional network of spindle-shaped cells was established. This new cell colony reproduced spheroids that functioned again as solitary sources of cellular proliferation. Addition of differentiation media resulted in lipid droplet deposition in the majority of generated cells, indicating the initial steps of adipogenic differentiation.
Conclusions: The authors noticed that crude, nonprocessed fat biopsy specimens do have considerable potential for future tissue engineering based applications, provided that the basic principles of developmental, cellular biology are respected. Spontaneous in vitro expansion of the stromal cells present in fat grafts within autologous and injectable matrices could create {\textacutedbl}off-the-shelf{\textacutedbl} therapies for reconstructive procedures. (Plast. Reconstr. Surg. 126: 1155, 2010.)},
  author       = {Stillaert, Filip and Abberton, Keren M and Keramidaris, Effie and Thompson, Erik W and Blondeel, Phillip and Morrison, Wayne A},
  issn         = {0032-1052},
  journal      = {PLASTIC AND RECONSTRUCTIVE SURGERY},
  keyword      = {DIFFERENTIATION,GROWTH,STEM-CELLS,EXTRACELLULAR-MATRIX,ACID-BASED SCAFFOLDS,TISSUE,ADIPOSE PRECURSOR CELLS,IMPLANTATION,MICE},
  language     = {eng},
  number       = {4},
  pages        = {1155--1162},
  title        = {Intrinsics and dynamics of fat grafts: an in vitro study},
  url          = {http://dx.doi.org/10.1097/PRS.0b013e3181ebe224},
  volume       = {126},
  year         = {2010},
}

Chicago
STILLAERT, FILIP, Keren M Abberton, Effie Keramidaris, Erik W Thompson, Phillip Blondeel, and Wayne A Morrison. 2010. “Intrinsics and Dynamics of Fat Grafts: An in Vitro Study.” Plastic and Reconstructive Surgery 126 (4): 1155–1162.
APA
STILLAERT, F., Abberton, K. M., Keramidaris, E., Thompson, E. W., Blondeel, P., & Morrison, W. A. (2010). Intrinsics and dynamics of fat grafts: an in vitro study. PLASTIC AND RECONSTRUCTIVE SURGERY, 126(4), 1155–1162.
Vancouver
1.
STILLAERT F, Abberton KM, Keramidaris E, Thompson EW, Blondeel P, Morrison WA. Intrinsics and dynamics of fat grafts: an in vitro study. PLASTIC AND RECONSTRUCTIVE SURGERY. 2010;126(4):1155–62.
MLA
STILLAERT, FILIP, Keren M Abberton, Effie Keramidaris, et al. “Intrinsics and Dynamics of Fat Grafts: An in Vitro Study.” PLASTIC AND RECONSTRUCTIVE SURGERY 126.4 (2010): 1155–1162. Print.