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Membrane filtration test for rapid presumptive differentiation of four Candida species

(1999) JOURNAL OF CLINICAL MICROBIOLOGY. 37(5). p.1498-1502
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Abstract
A rapid enzymatic two-step test for the presumptive differentiation of four Candida species commonly occurring in various clinical samples is described. The technique involves membrane filtration of a liquid sample, followed by preincubation of the membrane filter on Sabouraud glucose agar supplemented with ticarcillin-clavulanic acid to yield microcolonies. In a separate assay step, parts of the filter are placed on absorbent pads impregnated;vith fluorogenic 4-methylumbelliferyl ( 4-MU) enzyme substrates (4-MU-N-acetyl-beta-D-galactosaminide, 4-MU-phosphate, 4-MU-pyrophosphate. and 4-MU-beta-D-galactoside) in combination with 0.1% digitonin acting as a membrane permeabilizer. The membrane Biter in contact with the assay medium is incubated to allow cleavage of the enzyme substrate, resulting in fluorescent microcolonies under long-wavelength UV light This approach, tested on 301 clinical samples, is able to presumptively differentiate C. albicans, C. glabrata, C. krusei, and C. tropicalis and to distinguish them from other Candida spp. in about 9 to 11 h. Overall agreement with the conventional methods of 94.4% tone Candida species present in the sample to 83.8% (multiple Candida spp. present) aas obtained, The false-negative rates, with reference to identification by traditional methods were 1.39 (single species) and 3.8% (multiple species).
Keywords
KRUSEI, PLATES, PROSTHESIS, ALBICANS, DETERIORATION, IDENTIFICATION, YEAST-CELLS, CHROMAGAR CANDIDA, AGAR, SYSTEM

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Chicago
BAUTERS, TIENEKE, Renaat Peleman, M Moerman, Hubert Vermeersch, Danny De Looze, Lucien Noens, and Hans Nelis. 1999. “Membrane Filtration Test for Rapid Presumptive Differentiation of Four Candida Species.” Journal of Clinical Microbiology 37 (5): 1498–1502.
APA
BAUTERS, T., Peleman, R., Moerman, M., Vermeersch, H., De Looze, D., Noens, L., & Nelis, H. (1999). Membrane filtration test for rapid presumptive differentiation of four Candida species. JOURNAL OF CLINICAL MICROBIOLOGY, 37(5), 1498–1502.
Vancouver
1.
BAUTERS T, Peleman R, Moerman M, Vermeersch H, De Looze D, Noens L, et al. Membrane filtration test for rapid presumptive differentiation of four Candida species. JOURNAL OF CLINICAL MICROBIOLOGY. 1999;37(5):1498–502.
MLA
BAUTERS, TIENEKE, Renaat Peleman, M Moerman, et al. “Membrane Filtration Test for Rapid Presumptive Differentiation of Four Candida Species.” JOURNAL OF CLINICAL MICROBIOLOGY 37.5 (1999): 1498–1502. Print.
@article{115548,
  abstract     = {A rapid enzymatic two-step test for the presumptive differentiation of four Candida species commonly occurring in various clinical samples is described. The technique involves membrane filtration of a liquid sample, followed by preincubation of the membrane filter on Sabouraud glucose agar supplemented with ticarcillin-clavulanic acid to yield microcolonies. In a separate assay step, parts of the filter are placed on absorbent pads impregnated;vith fluorogenic 4-methylumbelliferyl ( 4-MU) enzyme substrates (4-MU-N-acetyl-beta-D-galactosaminide, 4-MU-phosphate, 4-MU-pyrophosphate. and 4-MU-beta-D-galactoside) in combination with 0.1\% digitonin acting as a membrane permeabilizer. The membrane Biter in contact with the assay medium is incubated to allow cleavage of the enzyme substrate, resulting in fluorescent microcolonies under long-wavelength UV light This approach, tested on 301 clinical samples, is able to presumptively differentiate C. albicans, C. glabrata, C. krusei, and C. tropicalis and to distinguish them from other Candida spp. in about 9 to 11 h. Overall agreement with the conventional methods of 94.4\% tone Candida species present in the sample to 83.8\% (multiple Candida spp. present) aas obtained, The false-negative rates, with reference to identification by traditional methods were 1.39 (single species) and 3.8\% (multiple species).},
  author       = {Bauters, Tieneke and Peleman, Renaat and Moerman, M and Vermeersch, Hubert and De Looze, Danny and Noens, Lucien and Nelis, Hans},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  keyword      = {KRUSEI,PLATES,PROSTHESIS,ALBICANS,DETERIORATION,IDENTIFICATION,YEAST-CELLS,CHROMAGAR CANDIDA,AGAR,SYSTEM},
  language     = {eng},
  number       = {5},
  pages        = {1498--1502},
  title        = {Membrane filtration test for rapid presumptive differentiation of four Candida species},
  url          = {http://jcm.asm.org/cgi/content/abstract/37/5/1498},
  volume       = {37},
  year         = {1999},
}

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