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Villin function in the organization of the actin cytoskeleton: correlation of in vivo effects to its biochemical activities in vitro

Evelyne Friederich, Katia Vancompernolle UGent, Daniel Louvard and Joël Vandekerckhove (1999) JOURNAL OF BIOLOGICAL CHEMISTRY. 274(38). p.26751-26760
abstract
Villin is an actin-binding protein of the intestinal brush border that bundles, nucleates, caps, and severs actin in a Ca2+-dependent manner in vitro, Villin induces the growth of microvilli in transfected cells, an activity that requires a carboxyl-terminally located KKEK motif. By combining cell transfection and biochemical assays, we show that the capacity of villin to induce growth of microvilli in cells correlates with its ability to bundle F-actin in vitro but not with its nucleating activity. In agreement with its importance for microfilament bundling in cells, the KKEK motif of the carboxyl-terminal F-actin-binding site is crucial for bundling in vitro, In addition, substitutions of basic residues in a second site, located in the amino-terminal portion of villin, impaired its activity in cells and reduced its binding to F-actin in the absence of Ca2+ as well as its bundling and severing activities in vitro. Altogether, these findings suggest that villin participates in the organization and stabilization of the brush border core bundle but does not initiate its assembly by nucleation of actin filaments.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MICROVILLUS CYTOSKELETON, F-ACTIN, BUNDLING PROTEIN, BINDING-PROTEINS, PHYSARUM ACTIN, GELSOLIN, DOMAINS, CALCIUM, SEQUENCE, CELLS
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
274
issue
38
pages
26751 - 26760
Web of Science type
Article
Web of Science id
000082570700018
ISSN
0021-9258
DOI
10.1074/jbc.274.38.26751
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
115429
handle
http://hdl.handle.net/1854/LU-115429
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:36:46
@article{115429,
  abstract     = {Villin is an actin-binding protein of the intestinal brush border that bundles, nucleates, caps, and severs actin in a Ca2+-dependent manner in vitro, Villin induces the growth of microvilli in transfected cells, an activity that requires a carboxyl-terminally located KKEK motif. By combining cell transfection and biochemical assays, we show that the capacity of villin to induce growth of microvilli in cells correlates with its ability to bundle F-actin in vitro but not with its nucleating activity. In agreement with its importance for microfilament bundling in cells, the KKEK motif of the carboxyl-terminal F-actin-binding site is crucial for bundling in vitro, In addition, substitutions of basic residues in a second site, located in the amino-terminal portion of villin, impaired its activity in cells and reduced its binding to F-actin in the absence of Ca2+ as well as its bundling and severing activities in vitro. Altogether, these findings suggest that villin participates in the organization and stabilization of the brush border core bundle but does not initiate its assembly by nucleation of actin filaments.},
  author       = {Friederich, Evelyne and Vancompernolle, Katia and Louvard, Daniel and Vandekerckhove, Jo{\"e}l},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {MICROVILLUS CYTOSKELETON,F-ACTIN,BUNDLING PROTEIN,BINDING-PROTEINS,PHYSARUM ACTIN,GELSOLIN,DOMAINS,CALCIUM,SEQUENCE,CELLS},
  language     = {eng},
  number       = {38},
  pages        = {26751--26760},
  title        = {Villin function in the organization of the actin cytoskeleton: correlation of in vivo effects to its biochemical activities in vitro},
  url          = {http://dx.doi.org/10.1074/jbc.274.38.26751},
  volume       = {274},
  year         = {1999},
}

Chicago
Friederich, Evelyne, Katia Vancompernolle, Daniel Louvard, and Joël Vandekerckhove. 1999. “Villin Function in the Organization of the Actin Cytoskeleton: Correlation of in Vivo Effects to Its Biochemical Activities in Vitro.” Journal of Biological Chemistry 274 (38): 26751–26760.
APA
Friederich, E., Vancompernolle, K., Louvard, D., & Vandekerckhove, J. (1999). Villin function in the organization of the actin cytoskeleton: correlation of in vivo effects to its biochemical activities in vitro. JOURNAL OF BIOLOGICAL CHEMISTRY, 274(38), 26751–26760.
Vancouver
1.
Friederich E, Vancompernolle K, Louvard D, Vandekerckhove J. Villin function in the organization of the actin cytoskeleton: correlation of in vivo effects to its biochemical activities in vitro. JOURNAL OF BIOLOGICAL CHEMISTRY. 1999;274(38):26751–60.
MLA
Friederich, Evelyne, Katia Vancompernolle, Daniel Louvard, et al. “Villin Function in the Organization of the Actin Cytoskeleton: Correlation of in Vivo Effects to Its Biochemical Activities in Vitro.” JOURNAL OF BIOLOGICAL CHEMISTRY 274.38 (1999): 26751–26760. Print.