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Advances in N-15-tracing experiments: new labelling and data analysis approaches

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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
To obtain an in-depth understanding of soil nitrogen dynamics, it is necessary to quantify a variety of simultaneously occurring gross nitrogen transformation processes. In order to do so, most studies apply N-15 in a disturbed soil-microbial-root system and quantify gross rates based on the principles of N-15 isotope dilution. However, this approach has several shortcomings. First, studying disturbed soil provides only limited information on in situ soil nitrogen dynamics. Secondly, the analytical data analysis allows the quantification of total production and consumption rates of the labelled pool, but does not provide information on process-specific transformation rates. Combining in situ N-15 isotope labelling over 1-2 weeks with numerical data analysis allows determining process-specific gross nitrogen transformations in undisturbed soils under field conditions in the presence of live roots and their associated microbial communities. This has the potential to increase our understanding of nitrogen dynamics in the soil environment.
Keywords
TROPICAL FOREST, POOL DILUTION, INTACT SOIL CORES, GROSS NITROGEN MINERALIZATION, ECOSYSTEM FUNCTION, PLANT, RHIZOSPHERE, RATES, N-15, IMMOBILIZATION, N-15 labelling, N-15 tracing, nitrogen transformation, soil nitrogen cycle

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Citation

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Chicago
Tobias, Rütting, Dries Huygens, Jeroen Staelens, Christoph Müller, and Pascal Boeckx. 2011. “Advances in N-15-tracing Experiments: New Labelling and Data Analysis Approaches.” Biochemical Society Transactions 39 (1): 279–283.
APA
Tobias, R., Huygens, D., Staelens, J., Müller, C., & Boeckx, P. (2011). Advances in N-15-tracing experiments: new labelling and data analysis approaches. BIOCHEMICAL SOCIETY TRANSACTIONS, 39(1), 279–283.
Vancouver
1.
Tobias R, Huygens D, Staelens J, Müller C, Boeckx P. Advances in N-15-tracing experiments: new labelling and data analysis approaches. BIOCHEMICAL SOCIETY TRANSACTIONS. 2011;39(1):279–83.
MLA
Tobias, Rütting, Dries Huygens, Jeroen Staelens, et al. “Advances in N-15-tracing Experiments: New Labelling and Data Analysis Approaches.” BIOCHEMICAL SOCIETY TRANSACTIONS 39.1 (2011): 279–283. Print.
@article{1154197,
  abstract     = {To obtain an in-depth understanding of soil nitrogen dynamics, it is necessary to quantify a variety of simultaneously occurring gross nitrogen transformation processes. In order to do so, most studies apply N-15 in a disturbed soil-microbial-root system and quantify gross rates based on the principles of N-15 isotope dilution. However, this approach has several shortcomings. First, studying disturbed soil provides only limited information on in situ soil nitrogen dynamics. Secondly, the analytical data analysis allows the quantification of total production and consumption rates of the labelled pool, but does not provide information on process-specific transformation rates. Combining in situ N-15 isotope labelling over 1-2 weeks with numerical data analysis allows determining process-specific gross nitrogen transformations in undisturbed soils under field conditions in the presence of live roots and their associated microbial communities. This has the potential to increase our understanding of nitrogen dynamics in the soil environment.},
  author       = {Tobias, R{\"u}tting and Huygens, Dries and Staelens, Jeroen and M{\"u}ller, Christoph and Boeckx, Pascal},
  issn         = {0300-5127},
  journal      = {BIOCHEMICAL SOCIETY TRANSACTIONS},
  keyword      = {TROPICAL FOREST,POOL DILUTION,INTACT SOIL CORES,GROSS NITROGEN MINERALIZATION,ECOSYSTEM FUNCTION,PLANT,RHIZOSPHERE,RATES,N-15,IMMOBILIZATION,N-15 labelling,N-15 tracing,nitrogen transformation,soil nitrogen cycle},
  language     = {eng},
  number       = {1},
  pages        = {279--283},
  title        = {Advances in N-15-tracing experiments: new labelling and data analysis approaches},
  url          = {http://dx.doi.org/10.1042/BST0390279},
  volume       = {39},
  year         = {2011},
}

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