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Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants

Lennert Steukers, Sarah Glorieux, Annelies Vandekerckhove UGent and Hans Nauwynck UGent (2010) Proceedings of the 35th annual international herpesvirus workshop.
abstract
Bovine herpes virus 1 (BoHV-1) belongs to the subfamily of the Alphaherpesvirinae. In general, alphaherpesviruses use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. BoHV-1 may replicate at both sites and causes two major clinical entities designated infectious bovine rhinotracheïtis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa where subtype 1.2 favors replication at the peripheral genital tract. However, some in vivo data are in contrast with that hypothesis. A thorough study of primary replication at both mucosae could elucidate this question. In the present study, we established a bovine respiratory and genital organ culture. Bovine respiratory and genital mucosal explants of the same animal were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral mucosal invasion was performed at 0, 24, 48 and 72 hours post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane (BM). BoHV-1 spreads plaquewise in the epithelium and penetrates the BM. Viral plaques could be observed at 24hpi for all isolates in both respiratory and genital mucosa explants with the plaque latitude increasing over time. At 24hpi, all the different subtypes spread more efficiently lateral in the epithelium of the genital tract compared to the respiratory tract. On the contrary, all BoHV-1 subtypes exhibit a more profound invasion capacity in the respiratory tract at 24h pi compared to the genital tract. These findings take the edge of the belief of the existence of specific mucosa tropisms off different BoHV-1 subtypes.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (meetingAbstract)
publication status
published
subject
in
Proceedings of the 35th annual international herpesvirus workshop
article number
abstract 2.23
conference name
35th Annual international Herpesvirus Workshop (IHW 2010)
conference location
Salt Lake City, UT, USA
conference start
2010-07-24
conference end
2010-07-29
language
English
UGent publication?
yes
classification
C3
copyright statement
I have transferred the copyright for this publication to the publisher
id
1153225
handle
http://hdl.handle.net/1854/LU-1153225
date created
2011-02-16 15:50:13
date last changed
2017-10-12 10:29:24
@inproceedings{1153225,
  abstract     = {Bovine herpes virus 1 (BoHV-1) belongs to the subfamily of the Alphaherpesvirinae. In general, alphaherpesviruses use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. BoHV-1 may replicate at both sites and causes two major clinical entities designated infectious bovine rhinotrache{\"i}tis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa where subtype 1.2 favors replication at the peripheral genital tract. However, some in vivo data are in contrast with that hypothesis. A thorough study of primary replication at both mucosae could elucidate this question. In the present study, we established a bovine respiratory and genital organ culture. Bovine respiratory and genital mucosal explants of the same animal were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral mucosal invasion was performed at 0, 24, 48 and 72 hours post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane (BM). BoHV-1 spreads plaquewise in the epithelium and penetrates the BM. Viral plaques could be observed at 24hpi for all isolates in both respiratory and genital mucosa explants with the plaque latitude increasing over time. At 24hpi, all the different subtypes spread more efficiently lateral in the epithelium of the genital tract compared to the respiratory tract. On the contrary, all BoHV-1 subtypes exhibit a more profound invasion capacity in the respiratory tract at 24h pi compared to the genital tract. These findings take the edge of the belief of the existence of specific mucosa tropisms off different BoHV-1 subtypes.},
  articleno    = {abstract 2.23},
  author       = {Steukers, Lennert and Glorieux, Sarah and Vandekerckhove, Annelies and Nauwynck, Hans},
  booktitle    = {Proceedings of the 35th annual international herpesvirus workshop},
  language     = {eng},
  location     = {Salt Lake City, UT, USA},
  title        = {Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants},
  year         = {2010},
}

Chicago
Steukers, Lennert, SARAH GLORIEUX, Annelies Vandekerckhove, and Hans Nauwynck. 2010. “Comparative Analysis of Replication Characteristics of Different BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants.” In Proceedings of the 35th Annual International Herpesvirus Workshop.
APA
Steukers, L., GLORIEUX, S., Vandekerckhove, A., & Nauwynck, H. (2010). Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants. Proceedings of the 35th annual international herpesvirus workshop. Presented at the 35th Annual international Herpesvirus Workshop (IHW 2010).
Vancouver
1.
Steukers L, GLORIEUX S, Vandekerckhove A, Nauwynck H. Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants. Proceedings of the 35th annual international herpesvirus workshop. 2010.
MLA
Steukers, Lennert, SARAH GLORIEUX, Annelies Vandekerckhove, et al. “Comparative Analysis of Replication Characteristics of Different BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants.” Proceedings of the 35th Annual International Herpesvirus Workshop. 2010. Print.