Advanced search
1 file | 111.91 KB

Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants

Lennert Steukers (UGent) , Sarah Glorieux (UGent) , Annelies Vandekerckhove (UGent) and Hans Nauwynck (UGent)
Author
Organization
Abstract
Bovine herpes virus 1 (BoHV-1) belongs to the subfamily of the Alphaherpesvirinae. In general, alphaherpesviruses use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. BoHV-1 may replicate at both sites and causes two major clinical entities designated infectious bovine rhinotracheïtis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa where subtype 1.2 favors replication at the peripheral genital tract. However, some in vivo data are in contrast with that hypothesis. A thorough study of primary replication at both mucosae could elucidate this question. In the present study, we established a bovine respiratory and genital organ culture. Bovine respiratory and genital mucosal explants of the same animal were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral mucosal invasion was performed at 0, 24, 48 and 72 hours post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane (BM). BoHV-1 spreads plaquewise in the epithelium and penetrates the BM. Viral plaques could be observed at 24hpi for all isolates in both respiratory and genital mucosa explants with the plaque latitude increasing over time. At 24hpi, all the different subtypes spread more efficiently lateral in the epithelium of the genital tract compared to the respiratory tract. On the contrary, all BoHV-1 subtypes exhibit a more profound invasion capacity in the respiratory tract at 24h pi compared to the genital tract. These findings take the edge of the belief of the existence of specific mucosa tropisms off different BoHV-1 subtypes.

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 111.91 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Steukers, Lennert, Sarah Glorieux, Annelies Vandekerckhove, and Hans Nauwynck. 2010. “Comparative Analysis of Replication Characteristics of Different BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants.” In Proceedings of the 35th Annual International Herpesvirus Workshop.
APA
Steukers, L., Glorieux, S., Vandekerckhove, A., & Nauwynck, H. (2010). Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants. Proceedings of the 35th annual international herpesvirus workshop. Presented at the 35th Annual international Herpesvirus Workshop (IHW 2010).
Vancouver
1.
Steukers L, Glorieux S, Vandekerckhove A, Nauwynck H. Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants. Proceedings of the 35th annual international herpesvirus workshop. 2010.
MLA
Steukers, Lennert, Sarah Glorieux, Annelies Vandekerckhove, et al. “Comparative Analysis of Replication Characteristics of Different BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants.” Proceedings of the 35th Annual International Herpesvirus Workshop. 2010. Print.
@inproceedings{1153225,
  abstract     = {Bovine herpes virus 1 (BoHV-1) belongs to the subfamily of the Alphaherpesvirinae. In general, alphaherpesviruses use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. BoHV-1 may replicate at both sites and causes two major clinical entities designated infectious bovine rhinotrache{\"i}tis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa where subtype 1.2 favors replication at the peripheral genital tract. However, some in vivo data are in contrast with that hypothesis. A thorough study of primary replication at both mucosae could elucidate this question. In the present study, we established a bovine respiratory and genital organ culture. Bovine respiratory and genital mucosal explants of the same animal were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral mucosal invasion was performed at 0, 24, 48 and 72 hours post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane (BM). BoHV-1 spreads plaquewise in the epithelium and penetrates the BM. Viral plaques could be observed at 24hpi for all isolates in both respiratory and genital mucosa explants with the plaque latitude increasing over time. At 24hpi, all the different subtypes spread more efficiently lateral in the epithelium of the genital tract compared to the respiratory tract. On the contrary, all BoHV-1 subtypes exhibit a more profound invasion capacity in the respiratory tract at 24h pi compared to the genital tract. These findings take the edge of the belief of the existence of specific mucosa tropisms off different BoHV-1 subtypes.},
  articleno    = {abstract 2.23},
  author       = {Steukers, Lennert and Glorieux, Sarah and Vandekerckhove, Annelies and Nauwynck, Hans},
  booktitle    = {Proceedings of the 35th annual international herpesvirus workshop},
  language     = {eng},
  location     = {Salt Lake City, UT, USA},
  title        = {Comparative analysis of replication characteristics of different BoHV-1 subtypes in bovine respiratory and genital mucosa explants},
  year         = {2010},
}