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Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment

Lennert Steukers (UGent) , Annelies Vandekerckhove (UGent) , Wim Van Den Broeck (UGent) , Sarah Glorieux (UGent) and Hans Nauwynck (UGent)
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Abstract
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.
Keywords
RESTRICTION-ENDONUCLEASE ANALYSIS, replication, quantitative analysis, subtype, PSEUDORABIES VIRUS, RHINOTRACHEITIS VIRUS, ESTROUS-CYCLE, HERPESVIRUS-1, INFECTION, STRAIN, CALVES, IBR, IDENTIFICATION, bohv-1, mucosa explant

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Chicago
Steukers, Lennert, Annelies Vandekerckhove, Wim Van Den Broeck, Sarah Glorieux, and Hans Nauwynck. 2011. “Comparative Analysis of Replication Characteristics of BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants: a Phylogenetic Enlightenment.” Veterinary Research 42.
APA
Steukers, L., Vandekerckhove, A., Van Den Broeck, W., Glorieux, S., & Nauwynck, H. (2011). Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment. VETERINARY RESEARCH, 42.
Vancouver
1.
Steukers L, Vandekerckhove A, Van Den Broeck W, Glorieux S, Nauwynck H. Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment. VETERINARY RESEARCH. 2011;42.
MLA
Steukers, Lennert, Annelies Vandekerckhove, Wim Van Den Broeck, et al. “Comparative Analysis of Replication Characteristics of BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants: a Phylogenetic Enlightenment.” VETERINARY RESEARCH 42 (2011): n. pag. Print.
@article{1153143,
  abstract     = {In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.},
  articleno    = {33},
  author       = {Steukers, Lennert and Vandekerckhove, Annelies and Van Den Broeck, Wim and Glorieux, Sarah and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {RESTRICTION-ENDONUCLEASE ANALYSIS,replication,quantitative analysis,subtype,PSEUDORABIES VIRUS,RHINOTRACHEITIS VIRUS,ESTROUS-CYCLE,HERPESVIRUS-1,INFECTION,STRAIN,CALVES,IBR,IDENTIFICATION,bohv-1,mucosa explant},
  language     = {eng},
  pages        = {11},
  title        = {Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-33},
  volume       = {42},
  year         = {2011},
}

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