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Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment

Lennert Steukers, Annelies Vandekerckhove UGent, Wim Van Den Broeck UGent, Sarah Glorieux and Hans Nauwynck UGent (2011) VETERINARY RESEARCH. 42.
abstract
In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RESTRICTION-ENDONUCLEASE ANALYSIS, replication, quantitative analysis, subtype, PSEUDORABIES VIRUS, RHINOTRACHEITIS VIRUS, ESTROUS-CYCLE, HERPESVIRUS-1, INFECTION, STRAIN, CALVES, IBR, IDENTIFICATION, bohv-1, mucosa explant
journal title
VETERINARY RESEARCH
Vet. Res.
volume
42
article number
33
pages
11 pages
Web of Science type
Article
Web of Science id
000290660800004
JCR category
VETERINARY SCIENCES
JCR impact factor
4.06 (2011)
JCR rank
1/141 (2011)
JCR quartile
1 (2011)
ISSN
0928-4249
DOI
10.1186/1297-9716-42-33
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1153143
handle
http://hdl.handle.net/1854/LU-1153143
date created
2011-02-16 15:27:52
date last changed
2018-02-19 14:10:55
@article{1153143,
  abstract     = {In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa whereas subtype 1.2 favors replication at the peripheral genital tract. However, some studies are in contrast with this hypothesis. A thorough study of primary replication at both mucosae could elucidate whether or not different BoHV-1 subtypes show differences in mucosa tropism. We established bovine respiratory and genital organ cultures with emphasis on maintenance of tissue morphology and viability during in vitro culture. In a next step, bovine respiratory and genital mucosa explants of the same animals were inoculated with several BoHV-1 subtypes. A quantitative analysis of viral invasion in the mucosa was performed at 0 h, 24 h, 48 h and 72 h post inoculation (pi) by measuring plaque latitude and penetration depth underneath the basement membrane. All BoHV-1 subtypes exhibited a more profound invasion capacity in respiratory tissue compared to that in genital tissue at 24 h pi. However, at 24 h pi plaque latitude was found to be larger in genital tissue compared to respiratory tissue and this for all subtypes. These similar findings among the different subtypes take the edge off the belief of the existence of specific mucosa tropisms of different BoHV-1 subtypes.},
  articleno    = {33},
  author       = {Steukers, Lennert and Vandekerckhove, Annelies and Van Den Broeck, Wim and Glorieux, Sarah and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {RESTRICTION-ENDONUCLEASE ANALYSIS,replication,quantitative analysis,subtype,PSEUDORABIES VIRUS,RHINOTRACHEITIS VIRUS,ESTROUS-CYCLE,HERPESVIRUS-1,INFECTION,STRAIN,CALVES,IBR,IDENTIFICATION,bohv-1,mucosa explant},
  language     = {eng},
  pages        = {11},
  title        = {Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-33},
  volume       = {42},
  year         = {2011},
}

Chicago
Steukers, Lennert, Annelies Vandekerckhove, Wim Van Den Broeck, Sarah Glorieux, and Hans Nauwynck. 2011. “Comparative Analysis of Replication Characteristics of BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants: a Phylogenetic Enlightenment.” Veterinary Research 42.
APA
Steukers, L., Vandekerckhove, A., Van Den Broeck, W., Glorieux, S., & Nauwynck, H. (2011). Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment. VETERINARY RESEARCH, 42.
Vancouver
1.
Steukers L, Vandekerckhove A, Van Den Broeck W, Glorieux S, Nauwynck H. Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment. VETERINARY RESEARCH. 2011;42.
MLA
Steukers, Lennert, Annelies Vandekerckhove, Wim Van Den Broeck, et al. “Comparative Analysis of Replication Characteristics of BoHV-1 Subtypes in Bovine Respiratory and Genital Mucosa Explants: a Phylogenetic Enlightenment.” VETERINARY RESEARCH 42 (2011): n. pag. Print.