Advanced search
1 file | 384.06 KB

Antibody responses to the host-protective taenia solium oncosphere protein TSOL18 in pigs are directed against conformational epitopes

(2010) PARASITE IMMUNOLOGY. 32(6). p.399-405
Author
Organization
Abstract
TSOL18 is a recombinant protein that has been shown in repeated experimental trials to be capable of protecting pigs against challenge infection with the cestode parasite Taenia solium. Antibodies raised by the vaccine are capable of killing the parasite in an in vitro culture and it is believed that antibody and complement-mediated killing of invading parasites is the major protective immune mechanism induced by vaccination with TSOL18. Investigations were undertaken to characterize whether the principal antibody specificities raised by TSOL18 in pigs were against linear or conformational determinants. TSOL18 was expressed in two truncated forms representing either the amino terminal portion or the carboxy terminal portion, with the two truncations overlapping in sequence by 25 amino acids. The original protein (designated TSOL18N-) and the two truncations (TSOL18N--1 and TSOL18N--2) were used in inhibition ELISA. TSOL18N- was shown to be capable of completely inhibiting the binding of pig anti-TSOL18N- antibodies to TSOL18N- in ELISA. However, neither TSOL18N--1 nor TSOL18N--2, either alone or when combined together, was capable of inhibiting any detectable amount of reactivity of pig anti-TSOL18N- antibodies with TSOL18N-. It is concluded that the dominant antibody specificities, and probably the host-protective specificities, of TSOL18 are conformational epitopes.
Keywords
vaccine, Taenia solium, WESTERN, OVIS, conformational epitope, INDUCTION, EG95, SAGINATA, DISEASE, RECOMBINANT ANTIGEN, HYDATID VACCINE, pig, cysticercosis, PORCINE CYSTICERCOSIS, TSOL18

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 384.06 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Assana, Emmanuel, CG Gauci, CT Kyngdon, AP Zoli, Pierre Dorny, S Geerts, and MW Lightowlers. 2010. “Antibody Responses to the Host-protective Taenia Solium Oncosphere Protein TSOL18 in Pigs Are Directed Against Conformational Epitopes.” Parasite Immunology 32 (6): 399–405.
APA
Assana, Emmanuel, Gauci, C., Kyngdon, C., Zoli, A., Dorny, P., Geerts, S., & Lightowlers, M. (2010). Antibody responses to the host-protective taenia solium oncosphere protein TSOL18 in pigs are directed against conformational epitopes. PARASITE IMMUNOLOGY, 32(6), 399–405.
Vancouver
1.
Assana E, Gauci C, Kyngdon C, Zoli A, Dorny P, Geerts S, et al. Antibody responses to the host-protective taenia solium oncosphere protein TSOL18 in pigs are directed against conformational epitopes. PARASITE IMMUNOLOGY. 2010;32(6):399–405.
MLA
Assana, Emmanuel, CG Gauci, CT Kyngdon, et al. “Antibody Responses to the Host-protective Taenia Solium Oncosphere Protein TSOL18 in Pigs Are Directed Against Conformational Epitopes.” PARASITE IMMUNOLOGY 32.6 (2010): 399–405. Print.
@article{1151205,
  abstract     = {TSOL18 is a recombinant protein that has been shown in repeated experimental trials to be capable of protecting pigs against challenge infection with the cestode parasite Taenia solium. Antibodies raised by the vaccine are capable of killing the parasite in an in vitro culture and it is believed that antibody and complement-mediated killing of invading parasites is the major protective immune mechanism induced by vaccination with TSOL18. Investigations were undertaken to characterize whether the principal antibody specificities raised by TSOL18 in pigs were against linear or conformational determinants. TSOL18 was expressed in two truncated forms representing either the amino terminal portion or the carboxy terminal portion, with the two truncations overlapping in sequence by 25 amino acids. The original protein (designated TSOL18N-) and the two truncations (TSOL18N--1 and TSOL18N--2) were used in inhibition ELISA. TSOL18N- was shown to be capable of completely inhibiting the binding of pig anti-TSOL18N- antibodies to TSOL18N- in ELISA. However, neither TSOL18N--1 nor TSOL18N--2, either alone or when combined together, was capable of inhibiting any detectable amount of reactivity of pig anti-TSOL18N- antibodies with TSOL18N-. It is concluded that the dominant antibody specificities, and probably the host-protective specificities, of TSOL18 are conformational epitopes.},
  author       = {Assana, Emmanuel and Gauci, CG and Kyngdon, CT and Zoli, AP and Dorny, Pierre and Geerts, S and Lightowlers, MW},
  issn         = {0141-9838},
  journal      = {PARASITE IMMUNOLOGY},
  keyword      = {vaccine,Taenia solium,WESTERN,OVIS,conformational epitope,INDUCTION,EG95,SAGINATA,DISEASE,RECOMBINANT ANTIGEN,HYDATID VACCINE,pig,cysticercosis,PORCINE CYSTICERCOSIS,TSOL18},
  language     = {eng},
  number       = {6},
  pages        = {399--405},
  title        = {Antibody responses to the host-protective taenia solium oncosphere protein TSOL18 in pigs are directed against conformational epitopes},
  url          = {http://dx.doi.org/10.1111/j.1365-3024.2009.01197.x},
  volume       = {32},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: