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Epicatechin induces NF-kappa B, activator rotein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells

Ana Belen Granado-Serrano, Maria Anegeles Martin, Guy Haegeman UGent, Luis Goya, Laura Bravo and Sonia Ramos (2010) BRITISH JOURNAL OF NUTRITION. 103(2). p.168-179
abstract
The dietary flavonoid epicatechin has been reported to exhibit a wide range of biological activities. The objective of the present study was to investigate the time-dependent regulation by epicatechin on the activity of the main transcription factors (NF-kappa B, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor (Nrf2)) related to antioxidant defence and survival and proliferation pathways in HepG2 cells. Treatment of cells with 10 mu M-epicatechin induced the NF-kappa B pathway in a time-dependent manner characterised by increased levels Of I kappa B kinase (IKK) and phosphorylated inhibitor Of kappa B subunit-a (p-I kappa B alpha) and proteolytic degradation Of I kappa B, which was consistent with an up-regulation of the NF-kappa B-binding activity. Time-dependent activation of the AP-1 pathway, in concert with enhanced c-Jun nuclear levels and induction of Nrf2 translocation and phosphorylation were also demonstrated. Additionally, epicatechin-induced NF-kappa B and Nrf2 were connected to reactive oxygen species intracellular levels and to the activation of cell survival and proliferation pathways, being phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) associated to Nrf2 modulation and ERK to NF-kappa B induction. These data suggest that the epicatechin-induced survival effect occurs by the induction of redox-sensitive transcription factors through a tight regulation of survival and proliferation pathways.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Nuclear factor-erythroid 2p45-related factor-2, CANCER CHEMOPREVENTION, RAS-TRANSFORMED CELLS, Activator protein-1, NITRIC-OXIDE SYNTHASE, GROWTH-FACTOR-BETA, OXIDATIVE-STRESS, GREEN TEA, ANTIOXIDANT RESPONSE ELEMENT, CHEMOPREVENTIVE COMPOUNDS, NF-kappa B signalling, Epicatechin, MOLECULAR-MECHANISM, HEME OXYGENASE-1
journal title
BRITISH JOURNAL OF NUTRITION
Br. J. Nutr.
volume
103
issue
2
pages
168 - 179
Web of Science type
Article
Web of Science id
000274762200004
JCR category
NUTRITION & DIETETICS
JCR impact factor
3.072 (2010)
JCR rank
19/70 (2010)
JCR quartile
2 (2010)
ISSN
0007-1145
DOI
10.1017/S0007114509991747
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1150822
handle
http://hdl.handle.net/1854/LU-1150822
date created
2011-02-15 11:02:39
date last changed
2011-02-22 11:39:35
@article{1150822,
  abstract     = {The dietary flavonoid epicatechin has been reported to exhibit a wide range of biological activities. The objective of the present study was to investigate the time-dependent regulation by epicatechin on the activity of the main transcription factors (NF-kappa B, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor (Nrf2)) related to antioxidant defence and survival and proliferation pathways in HepG2 cells. Treatment of cells with 10 mu M-epicatechin induced the NF-kappa B pathway in a time-dependent manner characterised by increased levels Of I kappa B kinase (IKK) and phosphorylated inhibitor Of kappa B subunit-a (p-I kappa B alpha) and proteolytic degradation Of I kappa B, which was consistent with an up-regulation of the NF-kappa B-binding activity. Time-dependent activation of the AP-1 pathway, in concert with enhanced c-Jun nuclear levels and induction of Nrf2 translocation and phosphorylation were also demonstrated. Additionally, epicatechin-induced NF-kappa B and Nrf2 were connected to reactive oxygen species intracellular levels and to the activation of cell survival and proliferation pathways, being phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) associated to Nrf2 modulation and ERK to NF-kappa B induction. These data suggest that the epicatechin-induced survival effect occurs by the induction of redox-sensitive transcription factors through a tight regulation of survival and proliferation pathways.},
  author       = {Granado-Serrano, Ana Belen and Martin, Maria Anegeles and Haegeman, Guy and Goya, Luis and Bravo, Laura and Ramos, Sonia},
  issn         = {0007-1145},
  journal      = {BRITISH JOURNAL OF NUTRITION},
  keyword      = {Nuclear factor-erythroid 2p45-related factor-2,CANCER CHEMOPREVENTION,RAS-TRANSFORMED CELLS,Activator protein-1,NITRIC-OXIDE SYNTHASE,GROWTH-FACTOR-BETA,OXIDATIVE-STRESS,GREEN TEA,ANTIOXIDANT RESPONSE ELEMENT,CHEMOPREVENTIVE COMPOUNDS,NF-kappa B signalling,Epicatechin,MOLECULAR-MECHANISM,HEME OXYGENASE-1},
  language     = {eng},
  number       = {2},
  pages        = {168--179},
  title        = {Epicatechin induces NF-kappa B, activator rotein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells},
  url          = {http://dx.doi.org/10.1017/S0007114509991747},
  volume       = {103},
  year         = {2010},
}

Chicago
Granado-Serrano, Ana Belen, Maria Anegeles Martin, Guy Haegeman, Luis Goya, Laura Bravo, and Sonia Ramos. 2010. “Epicatechin Induces NF-kappa B, Activator Rotein-1 (AP-1) and Nuclear Transcription Factor Erythroid 2p45-related Factor-2 (Nrf2) via Phosphatidylinositol-3-kinase/protein Kinase B (PI3K/AKT) and Extracellular Regulated Kinase (ERK) Signalling in HepG2 Cells.” British Journal of Nutrition 103 (2): 168–179.
APA
Granado-Serrano, A. B., Martin, M. A., Haegeman, G., Goya, L., Bravo, L., & Ramos, S. (2010). Epicatechin induces NF-kappa B, activator rotein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells. BRITISH JOURNAL OF NUTRITION, 103(2), 168–179.
Vancouver
1.
Granado-Serrano AB, Martin MA, Haegeman G, Goya L, Bravo L, Ramos S. Epicatechin induces NF-kappa B, activator rotein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells. BRITISH JOURNAL OF NUTRITION. 2010;103(2):168–79.
MLA
Granado-Serrano, Ana Belen, Maria Anegeles Martin, Guy Haegeman, et al. “Epicatechin Induces NF-kappa B, Activator Rotein-1 (AP-1) and Nuclear Transcription Factor Erythroid 2p45-related Factor-2 (Nrf2) via Phosphatidylinositol-3-kinase/protein Kinase B (PI3K/AKT) and Extracellular Regulated Kinase (ERK) Signalling in HepG2 Cells.” BRITISH JOURNAL OF NUTRITION 103.2 (2010): 168–179. Print.