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Hunting for serine 276-phosphorylated p65

Anneleen Spooren UGent, Krzysztof Kolmus UGent, Linda Vermeulen UGent, Karlien Van Wesemael UGent, Guy Haegeman UGent and Sarah Gerlo UGent (2010) JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.
abstract
The transcription factor nuclear factor kappaB (NF-kappa B) is one of the central mediators of inflammatory gene expression. Several posttranslational modifications of NF-kappa B, regulating its transactivation ability, have been described. Especially phosphorylation of the NF-kappa B subunit p65 has been investigated in depth and several commercial phosphospecific antibodies, targeting selected p65 residues, are available. One of the p65 residues, that is subject to phosphorylation by protein kinase A (PKA) as well as by mitogen-stimulated kinase-1 (MSK-1), is the serine at position 276. Here, we have performed a detailed analysis of the performance of the most commonly used commercial anti-P-p65 Ser276 antibodies. Our findings indicate that at least three widely used anti-P-p65 Ser276 antibodies do not detect p65 in vivo via Western Blot, but instead crossreact with PKA-regulated proteins. As PKA is one of the main kinases responsible for phosphorylation of p65 at Ser276, this observation warrants cautious interpretation of data generated using the tested antibodies.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INFLAMMATION, SUBUNIT, SEQUENCE, PHOSPHORYLATION, NUCLEAR-FACTOR, TRANSCRIPTIONAL ACTIVITY, NF-KAPPA-B
journal title
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
J. Biomed. Biotechnol.
article_number
275892
pages
9 pages
Web of Science type
Article
Web of Science id
000276209600001
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
1.225 (2010)
JCR rank
73/105 (2010)
JCR quartile
3 (2010)
ISSN
1110-7243
DOI
10.1155/2010/275892
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1150792
handle
http://hdl.handle.net/1854/LU-1150792
date created
2011-02-15 11:02:39
date last changed
2011-02-22 10:54:09
@article{1150792,
  abstract     = {The transcription factor nuclear factor kappaB (NF-kappa B) is one of the central mediators of inflammatory gene expression. Several posttranslational modifications of NF-kappa B, regulating its transactivation ability, have been described. Especially phosphorylation of the NF-kappa B subunit p65 has been investigated in depth and several commercial phosphospecific antibodies, targeting selected p65 residues, are available. One of the p65 residues, that is subject to phosphorylation by protein kinase A (PKA) as well as by mitogen-stimulated kinase-1 (MSK-1), is the serine at position 276. Here, we have performed a detailed analysis of the performance of the most commonly used commercial anti-P-p65 Ser276 antibodies. Our findings indicate that at least three widely used anti-P-p65 Ser276 antibodies do not detect p65 in vivo via Western Blot, but instead crossreact with PKA-regulated proteins. As PKA is one of the main kinases responsible for phosphorylation of p65 at Ser276, this observation warrants cautious interpretation of data generated using the tested antibodies.},
  articleno    = {275892},
  author       = {Spooren, Anneleen and Kolmus, Krzysztof and Vermeulen, Linda and Van Wesemael, Karlien and Haegeman, Guy and Gerlo, Sarah},
  issn         = {1110-7243},
  journal      = {JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY},
  keyword      = {INFLAMMATION,SUBUNIT,SEQUENCE,PHOSPHORYLATION,NUCLEAR-FACTOR,TRANSCRIPTIONAL ACTIVITY,NF-KAPPA-B},
  language     = {eng},
  pages        = {9},
  title        = {Hunting for serine 276-phosphorylated p65},
  url          = {http://dx.doi.org/10.1155/2010/275892},
  year         = {2010},
}

Chicago
Spooren, Anneleen, Krzysztof Kolmus, Linda Vermeulen, Karlien Van Wesemael, Guy Haegeman, and Sarah Gerlo. 2010. “Hunting for Serine 276-phosphorylated P65.” Journal of Biomedicine and Biotechnology.
APA
Spooren, A., Kolmus, K., Vermeulen, L., Van Wesemael, K., Haegeman, G., & Gerlo, S. (2010). Hunting for serine 276-phosphorylated p65. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.
Vancouver
1.
Spooren A, Kolmus K, Vermeulen L, Van Wesemael K, Haegeman G, Gerlo S. Hunting for serine 276-phosphorylated p65. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. 2010;
MLA
Spooren, Anneleen, Krzysztof Kolmus, Linda Vermeulen, et al. “Hunting for Serine 276-phosphorylated P65.” JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2010): n. pag. Print.