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Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e

Kim Nguyen Oanh Thi, Khong Nguyen Viet, Ngoc Do Thuy, Bruno Goddeeris UGent and Henri De Greve (2010) TROPICAL ANIMAL HEALTH AND PRODUCTION. 42(8). p.1797-1804
abstract
Edema disease (ED) is a common fatal disease in newly weaned piglets. To develop an effective control program for ED, we carried out a study to better understand the incidence and spread of the disease and the characteristics of the causative agent. In our study, 69 Escherichia coli strains, isolated from 92 piglets showing clinical signs of ED from 13 provinces in northern Vietnam, were positive for both the VT2e toxin and the F18 major fimbrial subunit gene fedA. Of these, 40 strains (58%) were positive for AIDA and 16 isolates carried one or more enterotoxins. Forty-six (67%) of the 69 VT2e(+)/F18(+) E. coli isolates belonged to classical serotypes (O139:K82, O141: K85, O138:K81, and O149:K91) while the remaining strains did not belong to the common serotypes in pig. Seropathotype 0139:K82(+)/VT2e(+)/F18(+)/AIDA(+) (21 isolates) was the most frequently detected ED-causing E. coli strain. High prevalence of resistance was observed to the common drugs of tetracycline, streptomycin, trimethoprim/sulfamethoxazole, amoxicillin/clavulanic acid, and spectinomycin. Multiple resistances were widely distributed with 84% of isolates resistant to five antibiotics. Sequence analysis demonstrated that the VT2e toxin is identical among E. coli strains causing ED in pig.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Escherichia coli, Edema disease, Virulence gene, Verotoxin 2e gene, Antimicrobial resistance, POLYMERASE-CHAIN-REACTION, TOXIN-II VARIANT, SHIGA TOXIN, POSTWEANING DIARRHEA, F-18 FIMBRIAE, MOLECULAR CHARACTERIZATION, EPITHELIAL-CELLS, PIGS, PREVALENCE, GENES
journal title
TROPICAL ANIMAL HEALTH AND PRODUCTION
Trop. Anim. Health Prod.
volume
42
issue
8
pages
1797 - 1804
Web of Science type
Article
Web of Science id
000283556000031
JCR category
VETERINARY SCIENCES
JCR impact factor
1.003 (2010)
JCR rank
59/145 (2010)
JCR quartile
2 (2010)
ISSN
0049-4747
DOI
10.1007/s11250-010-9639-6
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1150615
handle
http://hdl.handle.net/1854/LU-1150615
date created
2011-02-15 10:33:48
date last changed
2011-02-15 15:12:59
@article{1150615,
  abstract     = {Edema disease (ED) is a common fatal disease in newly weaned piglets. To develop an effective control program for ED, we carried out a study to better understand the incidence and spread of the disease and the characteristics of the causative agent. In our study, 69 Escherichia coli strains, isolated from 92 piglets showing clinical signs of ED from 13 provinces in northern Vietnam, were positive for both the VT2e toxin and the F18 major fimbrial subunit gene fedA. Of these, 40 strains (58\%) were positive for AIDA and 16 isolates carried one or more enterotoxins. Forty-six (67\%) of the 69 VT2e(+)/F18(+) E. coli isolates belonged to classical serotypes (O139:K82, O141: K85, O138:K81, and O149:K91) while the remaining strains did not belong to the common serotypes in pig. Seropathotype 0139:K82(+)/VT2e(+)/F18(+)/AIDA(+) (21 isolates) was the most frequently detected ED-causing E. coli strain. High prevalence of resistance was observed to the common drugs of tetracycline, streptomycin, trimethoprim/sulfamethoxazole, amoxicillin/clavulanic acid, and spectinomycin. Multiple resistances were widely distributed with 84\% of isolates resistant to five antibiotics. Sequence analysis demonstrated that the VT2e toxin is identical among E. coli strains causing ED in pig.},
  author       = {Thi, Kim Nguyen Oanh and Viet, Khong Nguyen and Thuy, Ngoc Do and Goddeeris, Bruno and De Greve, Henri},
  issn         = {0049-4747},
  journal      = {TROPICAL ANIMAL HEALTH AND PRODUCTION},
  keyword      = {Escherichia coli,Edema disease,Virulence gene,Verotoxin 2e gene,Antimicrobial resistance,POLYMERASE-CHAIN-REACTION,TOXIN-II VARIANT,SHIGA TOXIN,POSTWEANING DIARRHEA,F-18 FIMBRIAE,MOLECULAR CHARACTERIZATION,EPITHELIAL-CELLS,PIGS,PREVALENCE,GENES},
  language     = {eng},
  number       = {8},
  pages        = {1797--1804},
  title        = {Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e},
  url          = {http://dx.doi.org/10.1007/s11250-010-9639-6},
  volume       = {42},
  year         = {2010},
}

Chicago
Thi, Kim Nguyen Oanh, Khong Nguyen Viet, Ngoc Do Thuy, Bruno Goddeeris, and Henri De Greve. 2010. “Escherichia Coli Strains Causing Edema Disease in Northern Vietnam Share an Identical Verotoxin 2e.” Tropical Animal Health and Production 42 (8): 1797–1804.
APA
Thi, K. N. O., Viet, K. N., Thuy, N. D., Goddeeris, B., & De Greve, H. (2010). Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e. TROPICAL ANIMAL HEALTH AND PRODUCTION, 42(8), 1797–1804.
Vancouver
1.
Thi KNO, Viet KN, Thuy ND, Goddeeris B, De Greve H. Escherichia coli strains causing edema disease in northern Vietnam share an identical verotoxin 2e. TROPICAL ANIMAL HEALTH AND PRODUCTION. 2010;42(8):1797–804.
MLA
Thi, Kim Nguyen Oanh, Khong Nguyen Viet, Ngoc Do Thuy, et al. “Escherichia Coli Strains Causing Edema Disease in Northern Vietnam Share an Identical Verotoxin 2e.” TROPICAL ANIMAL HEALTH AND PRODUCTION 42.8 (2010): 1797–1804. Print.