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Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

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Abstract
Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P > 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P < 0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair (k - 0.1 - 0.4) with significantly higher sensitivity by PCR than other tests.
Keywords
Horses, Dourine, Prevalence and test agreement, TRYPANOSOMA-EVANSI INFECTION, WATER-BUFFALOS, EQUIPERDUM, TRYPANOZOON, PREVALENCE

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Chicago
Gari, Fikru Regassa, Hagos Ashenafi, Alemu Tola, Bruno Goddeeris, and Filip Claes. 2010. “Comparative Diagnosis of Parasitological, Serological, and Molecular Tests in Dourine-suspected Horses.” Tropical Animal Health and Production 42 (8): 1649–1654.
APA
Gari, F. R., Ashenafi, H., Tola, A., Goddeeris, B., & Claes, F. (2010). Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses. TROPICAL ANIMAL HEALTH AND PRODUCTION, 42(8), 1649–1654.
Vancouver
1.
Gari FR, Ashenafi H, Tola A, Goddeeris B, Claes F. Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses. TROPICAL ANIMAL HEALTH AND PRODUCTION. 2010;42(8):1649–54.
MLA
Gari, Fikru Regassa, Hagos Ashenafi, Alemu Tola, et al. “Comparative Diagnosis of Parasitological, Serological, and Molecular Tests in Dourine-suspected Horses.” TROPICAL ANIMAL HEALTH AND PRODUCTION 42.8 (2010): 1649–1654. Print.
@article{1150568,
  abstract     = {Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6\%, 36.7\%, and 47.6\% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6\% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6\% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P {\textrangle} 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P {\textlangle} 0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair (k - 0.1 - 0.4) with significantly higher sensitivity by PCR than other tests.},
  author       = {Gari, Fikru Regassa and Ashenafi, Hagos and Tola, Alemu and Goddeeris, Bruno and Claes, Filip},
  issn         = {0049-4747},
  journal      = {TROPICAL ANIMAL HEALTH AND PRODUCTION},
  keyword      = {Horses,Dourine,Prevalence and test agreement,TRYPANOSOMA-EVANSI INFECTION,WATER-BUFFALOS,EQUIPERDUM,TRYPANOZOON,PREVALENCE},
  language     = {eng},
  number       = {8},
  pages        = {1649--1654},
  title        = {Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses},
  url          = {http://dx.doi.org/10.1007/s11250-010-9615-1},
  volume       = {42},
  year         = {2010},
}

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