Ghent University Academic Bibliography

Advanced

Genetic dissection of the Ralstonia solanacearum hrp gene cluster reveals that the HrpV and HrpX proteins are required for Hrp pilus assembly

Frédérique Van Gijsegem, Jacques Vasse, Riet De Rycke UGent, Philippe Castello and Christian Boucher (2002) MOLECULAR MICROBIOLOGY. 44(4). p.935-946
abstract
In both plant and mammalian Gram-negative pathogenic bacteria, type III secretion systems (TTSSs) play a crucial role in interactions with the host. All these systems share conserved proteins (called Hrc in plant pathogens), but each bacterium also produces a variable number of additional type III proteins either unique or with counterparts only in a limited number of related systems. In order to investigate the role of the different proteins encoded by the hrp gene cluster of the phytopathogenic bacterium Ralstonia solanacearum , non-polar mutants in all hrp genes (except for hrcQ) were analysed for their interactions with plants, their ability to secrete the PopA protein and their production of the Hrp pilus. In addition to Hrc proteins and the HrpY major component of the Hrp pilus, four additional Hrp proteins are indispensable for type III secretion and for interactions with plants. We also provide evidence that hrpV and hrpX mutants can still target the HrpY pilin outside the bacterial cell but are impaired in the production of Hrp pili, indicating that HrpV and HrpX proteins are involved in the assembly of this appendage.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLANT-CELLS, EPITHELIAL-CELLS, PATHOGENICITY FACTOR, AVR PROTEINS, OUTER-MEMBRANE, GRAM-NEGATIVE BACTERIA, PSEUDOMONAS-SOLANACEARUM, III SECRETION PATHWAY, SYRINGAE, LOCALIZATION
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
44
issue
4
pages
935 - 946
Web of Science type
Article
Web of Science id
000175531300004
JCR category
MICROBIOLOGY
JCR impact factor
5.832 (2002)
JCR rank
7/81 (2002)
JCR quartile
1 (2002)
ISSN
0950-382X
DOI
10.1046/j.1365-2958.2002.02936.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1146197
handle
http://hdl.handle.net/1854/LU-1146197
date created
2011-02-10 19:41:58
date last changed
2012-09-19 14:05:53
@article{1146197,
  abstract     = {In both plant and mammalian Gram-negative pathogenic bacteria, type III secretion systems (TTSSs) play a crucial role in interactions with the host. All these systems share conserved proteins (called Hrc in plant pathogens), but each bacterium also produces a variable number of additional type III proteins either unique or with counterparts only in a limited number of related systems. In order to investigate the role of the different proteins encoded by the hrp gene cluster of the phytopathogenic bacterium Ralstonia solanacearum , non-polar mutants in all hrp genes (except for hrcQ) were analysed for their interactions with plants, their ability to secrete the PopA protein and their production of the Hrp pilus. In addition to Hrc proteins and the HrpY major component of the Hrp pilus, four additional Hrp proteins are indispensable for type III secretion and for interactions with plants. We also provide evidence that hrpV and hrpX mutants can still target the HrpY pilin outside the bacterial cell but are impaired in the production of Hrp pili, indicating that HrpV and HrpX proteins are involved in the assembly of this appendage.},
  author       = {Van Gijsegem, Fr{\'e}d{\'e}rique and Vasse, Jacques and De Rycke, Riet and Castello, Philippe and Boucher, Christian},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {PLANT-CELLS,EPITHELIAL-CELLS,PATHOGENICITY FACTOR,AVR PROTEINS,OUTER-MEMBRANE,GRAM-NEGATIVE BACTERIA,PSEUDOMONAS-SOLANACEARUM,III SECRETION PATHWAY,SYRINGAE,LOCALIZATION},
  language     = {eng},
  number       = {4},
  pages        = {935--946},
  title        = {Genetic dissection of the Ralstonia solanacearum hrp gene cluster reveals that the HrpV and HrpX proteins are required for Hrp pilus assembly},
  url          = {http://dx.doi.org/10.1046/j.1365-2958.2002.02936.x},
  volume       = {44},
  year         = {2002},
}

Chicago
Van Gijsegem, Frédérique, Jacques Vasse, Riet De Rycke, Philippe Castello, and Christian Boucher. 2002. “Genetic Dissection of the Ralstonia Solanacearum Hrp Gene Cluster Reveals That the HrpV and HrpX Proteins Are Required for Hrp Pilus Assembly.” Molecular Microbiology 44 (4): 935–946.
APA
Van Gijsegem, F., Vasse, J., De Rycke, R., Castello, P., & Boucher, C. (2002). Genetic dissection of the Ralstonia solanacearum hrp gene cluster reveals that the HrpV and HrpX proteins are required for Hrp pilus assembly. MOLECULAR MICROBIOLOGY, 44(4), 935–946.
Vancouver
1.
Van Gijsegem F, Vasse J, De Rycke R, Castello P, Boucher C. Genetic dissection of the Ralstonia solanacearum hrp gene cluster reveals that the HrpV and HrpX proteins are required for Hrp pilus assembly. MOLECULAR MICROBIOLOGY. 2002;44(4):935–46.
MLA
Van Gijsegem, Frédérique, Jacques Vasse, Riet De Rycke, et al. “Genetic Dissection of the Ralstonia Solanacearum Hrp Gene Cluster Reveals That the HrpV and HrpX Proteins Are Required for Hrp Pilus Assembly.” MOLECULAR MICROBIOLOGY 44.4 (2002): 935–946. Print.