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Functional analysis of Cyclophilin B reveals a cell cycle dependent nucleolar pattern with a possible role in rDNA transcription

Birger Dieriks UGent, Anneleen Cottyn UGent, Winnok De Vos UGent and Patric Van Oostveldt UGent (2010) Keystone symposia on nuclear receptors : signaling, gene regulation and cancer, Abstracts.
abstract
Cyclophilin B (CypB) belongs to the family of Cyclophilins, ubiquitously distributed proteins with confirmed functions in nucleus, cytoplasm and as intercellular communicator. CypB is able to combine these functions through the presence of a N-terminal nuclear localization signal (NLS) that becomes active after selective removal of the first 33 amino acids. Immunofluorescent staining in human dermal fibroblasts revealed that CypB expression follows a cell cycle dependent pattern with a particular preference for localization at the nucleoli. The fusion of different deletion mutants with GFP allowed reconstruction of the various described phenotypes found in literature. We found that CypB contains in fact a nucleolar localization signal that allows efficient relocation to the nucleoli in a cell cycle dependent manner. CypB revealed a high preference for the fibrillar centers (FC’s). In these FC’s CypB colocalized with factors involved in rDNA transcription, such as RNA-polymerase, Upstream binding factor-1 (UBF) and fibrillarin. This protein association was maintained after disruption of the nuclear architecture with adenosine analog DRB and became associated with cajal bodies. We propose that CypB interacts with these proteins and is involved in the rDNA transcription during the transcriptionally more active phases of the cell cycle. This work is supported by a BOF grant provided by University Gent, Belgium
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
Cyclophilin, Nucleolus, rRNA, NLS-signal
in
Keystone symposia on nuclear receptors : signaling, gene regulation and cancer, Abstracts
conference name
Keystone Symposia on Nuclear Receptors : Signaling, Gene Regulation and Cancer
conference location
Keystone, CO, USA
conference start
2010-03-21
conference end
2010-03-26
language
English
UGent publication?
yes
classification
C3
id
1141600
handle
http://hdl.handle.net/1854/LU-1141600
date created
2011-02-07 16:19:29
date last changed
2011-05-05 10:19:49
@inproceedings{1141600,
  abstract     = {Cyclophilin B (CypB) belongs to the family of Cyclophilins, ubiquitously distributed proteins with confirmed functions in nucleus, cytoplasm and as intercellular communicator. CypB is able to combine these functions through the presence of a N-terminal nuclear localization signal (NLS) that becomes active after selective removal of the first 33 amino acids. Immunofluorescent staining in human dermal fibroblasts revealed that CypB expression follows a cell cycle dependent pattern with a particular preference for localization at the nucleoli. The fusion of different deletion mutants with GFP allowed reconstruction of the various described phenotypes found in literature. We found that CypB contains in fact a nucleolar localization signal that allows efficient relocation to the nucleoli in a cell cycle dependent manner. CypB revealed a high preference for the fibrillar centers (FC{\textquoteright}s). In these FC{\textquoteright}s CypB colocalized with factors involved in rDNA transcription, such as RNA-polymerase, Upstream binding factor-1 (UBF) and fibrillarin. This protein association was maintained after disruption of the nuclear architecture with adenosine analog DRB and became associated with cajal bodies. We propose that CypB interacts with these proteins and is involved in the rDNA transcription during the transcriptionally more active phases of the cell cycle. This work is supported by a BOF grant provided by University Gent, Belgium},
  author       = {Dieriks, Birger and Cottyn, Anneleen and De Vos, Winnok and Van Oostveldt, Patric},
  booktitle    = {Keystone symposia on nuclear receptors : signaling, gene regulation and cancer, Abstracts},
  keyword      = {Cyclophilin,Nucleolus,rRNA,NLS-signal},
  language     = {eng},
  location     = {Keystone, CO, USA},
  title        = {Functional analysis of Cyclophilin B reveals a cell cycle dependent nucleolar pattern with a possible role in rDNA transcription},
  year         = {2010},
}

Chicago
Dieriks, Birger, Anneleen Cottyn, Winnok De Vos, and Patric Van Oostveldt. 2010. “Functional Analysis of Cyclophilin B Reveals a Cell Cycle Dependent Nucleolar Pattern with a Possible Role in rDNA Transcription.” In Keystone Symposia on Nuclear Receptors : Signaling, Gene Regulation and Cancer, Abstracts.
APA
Dieriks, B., Cottyn, A., De Vos, W., & Van Oostveldt, P. (2010). Functional analysis of Cyclophilin B reveals a cell cycle dependent nucleolar pattern with a possible role in rDNA transcription. Keystone symposia on nuclear receptors : signaling, gene regulation and cancer, Abstracts. Presented at the Keystone Symposia on Nuclear Receptors : Signaling, Gene Regulation and Cancer.
Vancouver
1.
Dieriks B, Cottyn A, De Vos W, Van Oostveldt P. Functional analysis of Cyclophilin B reveals a cell cycle dependent nucleolar pattern with a possible role in rDNA transcription. Keystone symposia on nuclear receptors : signaling, gene regulation and cancer, Abstracts. 2010.
MLA
Dieriks, Birger, Anneleen Cottyn, Winnok De Vos, et al. “Functional Analysis of Cyclophilin B Reveals a Cell Cycle Dependent Nucleolar Pattern with a Possible Role in rDNA Transcription.” Keystone Symposia on Nuclear Receptors : Signaling, Gene Regulation and Cancer, Abstracts. 2010. Print.