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Analysis of radiation-induced DNA-damage generated by X-rays

Birger Dieriks UGent, Winnok De Vos UGent and Patric Van Oostveldt UGent (2008) Annual symposium : DNA damage : from causes to cures, Abstracts.
abstract
Ionizing radiation poses a severe threat to chromosomal integrity and genome stability by inducing both single stranded and double stranded breaks. When exposed to radiation, cells rapidly phosphorylate the nucleosomal histone protein H2AX at sites surrounding double-stranded breaks (DSB). The phosphorylation of H2AX (γH2AX) functions as a signal enhancer for DSB-repair. XRCC1 is a critical scaffold protein that orchestrates efficient single-strand break repair (SSB) by coordinating the enzymatic processing in base excision repair. Using XRCC1 as a representative of global SSB- and γH2AX as marker for DSB-repair, we assessed the global DNA repair response in human primary fibroblasts and cell lines after exposure to ionizing radiation. Using immunocytochemistry and automated high-content cytometry we were able to extract statistical information on global population response as well as individual variations at the level of the single cell. Cell lines were found to have higher basal levels of both DNA damage markers than fibroblasts. In fibroblasts, a cell cycle specific pattern for γH2AX was observed: γH2AX is significantly upregulated during S-phase. Although XRCC1 showed no cell cycle dependency, a distinct dose-dependent relocation to the nucleoli was observed 4-6 hours after X-ray exposure. We hypothesize that these nucleoli function as factories where XRCC1 is processed.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Annual symposium : DNA damage : from causes to cures, Abstracts
conference name
Annual Symposium : DNA damage : from causes to cures
conference location
Cambridge, UK
conference start
2008-12-15
conference end
2008-12-17
language
English
UGent publication?
yes
classification
C3
id
1141585
handle
http://hdl.handle.net/1854/LU-1141585
date created
2011-02-07 16:14:39
date last changed
2016-12-19 15:35:22
@inproceedings{1141585,
  abstract     = {Ionizing radiation poses a severe threat to chromosomal integrity and genome stability by inducing both single stranded and double stranded breaks. When exposed to radiation, cells rapidly phosphorylate the nucleosomal histone protein H2AX at sites surrounding double-stranded breaks (DSB). The phosphorylation of H2AX (\ensuremath{\gamma}H2AX) functions as a signal enhancer for DSB-repair. XRCC1 is a critical scaffold protein that orchestrates efficient single-strand break repair (SSB) by coordinating the enzymatic processing in base excision repair. Using XRCC1 as a representative of global SSB- and \ensuremath{\gamma}H2AX as marker for DSB-repair, we assessed the global DNA repair response in human primary fibroblasts and cell lines after exposure to ionizing radiation. Using immunocytochemistry and automated high-content cytometry we were able to extract statistical information on global population response as well as individual variations at the level of the single cell. Cell lines were found to have higher basal levels of both DNA damage markers than fibroblasts. In fibroblasts, a cell cycle specific pattern for \ensuremath{\gamma}H2AX was observed: \ensuremath{\gamma}H2AX is significantly upregulated during S-phase. Although XRCC1 showed no cell cycle dependency, a distinct dose-dependent relocation to the nucleoli was observed 4-6 hours after X-ray exposure. We hypothesize that these nucleoli function as factories where XRCC1 is processed.},
  author       = {Dieriks, Birger and De Vos, Winnok and Van Oostveldt, Patric},
  booktitle    = {Annual symposium : DNA damage : from causes to cures, Abstracts},
  language     = {eng},
  location     = {Cambridge, UK},
  title        = {Analysis of radiation-induced DNA-damage generated by X-rays},
  year         = {2008},
}

Chicago
Dieriks, Birger, Winnok De Vos, and Patric Van Oostveldt. 2008. “Analysis of Radiation-induced DNA-damage Generated by X-rays.” In Annual Symposium : DNA Damage : from Causes to Cures, Abstracts.
APA
Dieriks, B., De Vos, W., & Van Oostveldt, P. (2008). Analysis of radiation-induced DNA-damage generated by X-rays. Annual symposium : DNA damage : from causes to cures, Abstracts. Presented at the Annual Symposium : DNA damage : from causes to cures.
Vancouver
1.
Dieriks B, De Vos W, Van Oostveldt P. Analysis of radiation-induced DNA-damage generated by X-rays. Annual symposium : DNA damage : from causes to cures, Abstracts. 2008.
MLA
Dieriks, Birger, Winnok De Vos, and Patric Van Oostveldt. “Analysis of Radiation-induced DNA-damage Generated by X-rays.” Annual Symposium : DNA Damage : from Causes to Cures, Abstracts. 2008. Print.