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Analysis of radiation-induced DNA-damage generated by X-rays

Birger Dieriks (UGent) , Winnok De Vos (UGent) and Patric Van Oostveldt (UGent)
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Abstract
Ionizing radiation poses a severe threat to chromosomal integrity and genome stability by inducing both single stranded and double stranded breaks. When exposed to radiation, cells rapidly phosphorylate the nucleosomal histone protein H2AX at sites surrounding double-stranded breaks (DSB). The phosphorylation of H2AX (γH2AX) functions as a signal enhancer for DSB-repair. XRCC1 is a critical scaffold protein that orchestrates efficient single-strand break repair (SSB) by coordinating the enzymatic processing in base excision repair. Using XRCC1 as a representative of global SSB- and γH2AX as marker for DSB-repair, we assessed the global DNA repair response in human primary fibroblasts and cell lines after exposure to ionizing radiation. Using immunocytochemistry and automated high-content cytometry we were able to extract statistical information on global population response as well as individual variations at the level of the single cell. Cell lines were found to have higher basal levels of both DNA damage markers than fibroblasts. In fibroblasts, a cell cycle specific pattern for γH2AX was observed: γH2AX is significantly upregulated during S-phase. Although XRCC1 showed no cell cycle dependency, a distinct dose-dependent relocation to the nucleoli was observed 4-6 hours after X-ray exposure. We hypothesize that these nucleoli function as factories where XRCC1 is processed.

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Chicago
Dieriks, Birger, Winnok De Vos, and Patric Van Oostveldt. 2008. “Analysis of Radiation-induced DNA-damage Generated by X-rays.” In Annual Symposium : DNA Damage : from Causes to Cures, Abstracts.
APA
Dieriks, B., De Vos, W., & Van Oostveldt, P. (2008). Analysis of radiation-induced DNA-damage generated by X-rays. Annual symposium : DNA damage : from causes to cures, Abstracts. Presented at the Annual Symposium : DNA damage : from causes to cures.
Vancouver
1.
Dieriks B, De Vos W, Van Oostveldt P. Analysis of radiation-induced DNA-damage generated by X-rays. Annual symposium : DNA damage : from causes to cures, Abstracts. 2008.
MLA
Dieriks, Birger, Winnok De Vos, and Patric Van Oostveldt. “Analysis of Radiation-induced DNA-damage Generated by X-rays.” Annual Symposium : DNA Damage : from Causes to Cures, Abstracts. 2008. Print.
@inproceedings{1141585,
  abstract     = {Ionizing radiation poses a severe threat to chromosomal integrity and genome stability by inducing both single stranded and double stranded breaks. When exposed to radiation, cells rapidly phosphorylate the nucleosomal histone protein H2AX at sites surrounding double-stranded breaks (DSB). The phosphorylation of H2AX (\ensuremath{\gamma}H2AX) functions as a signal enhancer for DSB-repair. XRCC1 is a critical scaffold protein that orchestrates efficient single-strand break repair (SSB) by coordinating the enzymatic processing in base excision repair. Using XRCC1 as a representative of global SSB- and \ensuremath{\gamma}H2AX as marker for DSB-repair, we assessed the global DNA repair response in human primary fibroblasts and cell lines after exposure to ionizing radiation. Using immunocytochemistry and automated high-content cytometry we were able to extract statistical information on global population response as well as individual variations at the level of the single cell. Cell lines were found to have higher basal levels of both DNA damage markers than fibroblasts. In fibroblasts, a cell cycle specific pattern for \ensuremath{\gamma}H2AX was observed: \ensuremath{\gamma}H2AX is significantly upregulated during S-phase. Although XRCC1 showed no cell cycle dependency, a distinct dose-dependent relocation to the nucleoli was observed 4-6 hours after X-ray exposure. We hypothesize that these nucleoli function as factories where XRCC1 is processed.},
  author       = {Dieriks, Birger and De Vos, Winnok and Van Oostveldt, Patric},
  booktitle    = {Annual symposium : DNA damage : from causes to cures, Abstracts},
  language     = {eng},
  location     = {Cambridge, UK},
  title        = {Analysis of radiation-induced DNA-damage generated by X-rays},
  year         = {2008},
}