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Nuclear localization of the p120ctn Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression

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Abstract
The Armadillo protein p120(ctn) associates with the cytoplasmic domain of cadherins and accumulates at cell-cell junctions. Particular Armadillo proteins such as beta-catenin and plakophilins show a partly nuclear location, suggesting gene-regulatory activities. For different human E-cadherin-negative carcinoma cancer cell lines we found expression of endogenous p120(ctn) in the nucleus. Expression of E-cadherin directed p120(ctn) out of the nucleus. Previously, we reported that the human p120(ctn) gene might encode up to 32 protein isoforms as products of alternative splicing. Overexpression of p120(ctn) isoforms B in various cell lines resulted in cytoplasmic immunopositivity but never in nuclear staining. In contrast, upon-expression of p120(ctn) cDNAs lacking exon B, the isoforms were detectable within both nuclei and cytoplasm. A putative nuclear export signal (NES) with a characteristic leucine-rich motif is encoded by exon B. This sequence element was shown to be required for nuclear export and to function autonomously when fused to a carrier protein and microinjected into cell nuclei. Moreover, the NES function of endogenously or exogenously expressed p120(ctn) isoforms B was sensitive to the nuclear export inhibitor leptomycin B. Expression of exogenous E-cadherin down-regulated nuclear p120(ctn) whereas activation of protein kinase C increased the level of nuclear p120(ctn). These results reveal molecular mechanisms controlling the subcellular distribution of p120(ctn).
Keywords
MOLECULES ALPHA-CATENINS, TRANSCRIPTION FACTOR LEF-1, HUMAN COLORECTAL-CANCER, SEGMENT POLARITY GENE, BETA-CATENIN, TYROSINE PHOSPHORYLATION, CELL-ADHESION, DESMOSOMAL PLAQUE, EPITHELIAL-CELLS, MAMMALIAN-CELLS

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Chicago
van Hengel, Jolanda, Peter Vanhoenacker, Katrien Staes, and Frans Van Roy. 1999. “Nuclear Localization of the P120ctn Armadillo-like Catenin Is Counteracted by a Nuclear Export Signal and by E-cadherin Expression.” Proceedings of the National Academy of Sciences of the United States of America 96 (14): 7980–7985.
APA
van Hengel, J., Vanhoenacker, P., Staes, K., & Van Roy, F. (1999). Nuclear localization of the p120ctn Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96(14), 7980–7985.
Vancouver
1.
van Hengel J, Vanhoenacker P, Staes K, Van Roy F. Nuclear localization of the p120ctn Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1999;96(14):7980–5.
MLA
van Hengel, Jolanda, Peter Vanhoenacker, Katrien Staes, et al. “Nuclear Localization of the P120ctn Armadillo-like Catenin Is Counteracted by a Nuclear Export Signal and by E-cadherin Expression.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 96.14 (1999): 7980–7985. Print.
@article{114026,
  abstract     = {The Armadillo protein p120(ctn) associates with the cytoplasmic domain of cadherins and accumulates at cell-cell junctions. Particular Armadillo proteins such as beta-catenin and plakophilins show a partly nuclear location, suggesting gene-regulatory activities. For different human E-cadherin-negative carcinoma cancer cell lines we found expression of endogenous p120(ctn) in the nucleus. Expression of E-cadherin directed p120(ctn) out of the nucleus. Previously, we reported that the human p120(ctn) gene might encode up to 32 protein isoforms as products of alternative splicing. Overexpression of p120(ctn) isoforms B in various cell lines resulted in cytoplasmic immunopositivity but never in nuclear staining. In contrast, upon-expression of p120(ctn) cDNAs lacking exon B, the isoforms were detectable within both nuclei and cytoplasm. A putative nuclear export signal (NES) with a characteristic leucine-rich motif is encoded by exon B. This sequence element was shown to be required for nuclear export and to function autonomously when fused to a carrier protein and microinjected into cell nuclei. Moreover, the NES function of endogenously or exogenously expressed p120(ctn) isoforms B was sensitive to the nuclear export inhibitor leptomycin B. Expression of exogenous E-cadherin down-regulated nuclear p120(ctn) whereas activation of protein kinase C increased the level of nuclear p120(ctn). These results reveal molecular mechanisms controlling the subcellular distribution of p120(ctn).},
  author       = {van Hengel, Jolanda and Vanhoenacker, Peter and Staes, Katrien and Van Roy, Frans},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keywords     = {MOLECULES ALPHA-CATENINS,TRANSCRIPTION FACTOR LEF-1,HUMAN COLORECTAL-CANCER,SEGMENT POLARITY GENE,BETA-CATENIN,TYROSINE PHOSPHORYLATION,CELL-ADHESION,DESMOSOMAL PLAQUE,EPITHELIAL-CELLS,MAMMALIAN-CELLS},
  language     = {eng},
  number       = {14},
  pages        = {7980--7985},
  title        = {Nuclear localization of the p120ctn Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression},
  url          = {http://dx.doi.org/10.1073/pnas.96.14.7980},
  volume       = {96},
  year         = {1999},
}

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