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Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria

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Abstract
Strains belonging to the Gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (TGGE), This method allows the sequence-specific separation of amplified fragments of 16S rRNA genes, A total of 115 reference strains representing the majority of the species of the genera Aeromicrobium, Agromyces, Arthrobacter, Aureobacterium, Cellulomonas, Cortobacterium, Nocardioides and Terrabacter were characterized. Depending on the genus investigated, the resolution limit of the technique appeared to be at the species or genus level or intermediate between the two. Aberrant TGGE profiles of strains within particular taxa revealed genomic heterogeneity and generic misclassification of nine strains studied. Beyond that, indications of 16S rRNA gene heterogeneity were found within the genomes of three Curtobacterium strains. The misclassifications revealed by TGGE were confirmed using whole-cell fatty acid methyl ester analysis and subsequent comparison with a database. TGGE has been demonstrated to be a useful tool in bacterial taxonomy.
Keywords
ACTINOBACTERIA, PCR, PROPOSAL, SEQUENCE, FRAGMENTS, IDENTIFICATION, DNA, COMB-NOV, 16S RIBOSOMAL-RNA, fatty acid methyl ester, 16S rDNA gene heterogeneity, temperature-gradient gel electrophoresis, coryneform bacteria, CELLULOMONAS

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Citation

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Chicago
Felske, Andreas, Marc Vancanneyt, Karel Kersters, and Antoon DL Akkermans. 1999. “Application of Temperature-gradient Gel Electrophoresis in Taxonomy of Coryneform Bacteria.” International Journal of Systematic Bacteriology 49 (1): 113–121.
APA
Felske, A., Vancanneyt, M., Kersters, K., & Akkermans, A. D. (1999). Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 49(1), 113–121.
Vancouver
1.
Felske A, Vancanneyt M, Kersters K, Akkermans AD. Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY. 1999;49(1):113–21.
MLA
Felske, Andreas, Marc Vancanneyt, Karel Kersters, et al. “Application of Temperature-gradient Gel Electrophoresis in Taxonomy of Coryneform Bacteria.” INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY 49.1 (1999): 113–121. Print.
@article{112949,
  abstract     = {Strains belonging to the Gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (TGGE), This method allows the sequence-specific separation of amplified fragments of 16S rRNA genes, A total of 115 reference strains representing the majority of the species of the genera Aeromicrobium, Agromyces, Arthrobacter, Aureobacterium, Cellulomonas, Cortobacterium, Nocardioides and Terrabacter were characterized. Depending on the genus investigated, the resolution limit of the technique appeared to be at the species or genus level or intermediate between the two. Aberrant TGGE profiles of strains within particular taxa revealed genomic heterogeneity and generic misclassification of nine strains studied. Beyond that, indications of 16S rRNA gene heterogeneity were found within the genomes of three Curtobacterium strains. The misclassifications revealed by TGGE were confirmed using whole-cell fatty acid methyl ester analysis and subsequent comparison with a database. TGGE has been demonstrated to be a useful tool in bacterial taxonomy.},
  author       = {Felske, Andreas and Vancanneyt, Marc and Kersters, Karel and Akkermans, Antoon DL},
  issn         = {0020-7713},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY},
  keyword      = {ACTINOBACTERIA,PCR,PROPOSAL,SEQUENCE,FRAGMENTS,IDENTIFICATION,DNA,COMB-NOV,16S RIBOSOMAL-RNA,fatty acid methyl ester,16S rDNA gene heterogeneity,temperature-gradient gel electrophoresis,coryneform bacteria,CELLULOMONAS},
  language     = {eng},
  number       = {1},
  pages        = {113--121},
  title        = {Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria},
  url          = {http://dx.doi.org/10.1099/00207713-49-1-113},
  volume       = {49},
  year         = {1999},
}

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