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Comparison of glycopeptide-resistant Enterococcus faecium isolates and glycopeptide resistance genes of human and animal origins

Patrick Descheemaeker, Sabine Chapelle, Luc Devriese UGent, Patrick Butaye UGent, Peter Vandamme UGent and Herman Goossens (1999) ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 43(8). p.2032-2037
abstract
One hundred thirty-two glycopeptide-resistant Enterococcus faecium (GREF) isolates from different hospitals and pig and poultry farms in Belgium were compared on the basis of (i) their antibiotic susceptibilities, (ii) their SmaI pulsed-field gel electrophoresis (PFGE) patterns, and (iii) the organization of their Tn1546 or related elements in order to detect possible phenotypic and genotypic relationships among both groups of isolates. Human and animal vanA-positive GREF isolates were found to have similar susceptibility patterns; they remained susceptible to gentamicin and were, in general, susceptible to ampicillin. PFGE demonstrated a very high degree of genomic heterogeneity in both groups of isolates, However, indistinguishable isolates were found within different farms or hospitals, and in two instances, epidemiologically unrelated pig and human isolates showed indistinguishable PFGE patterns. In total, eight different transposon types were identified, and all were related to the prototype transposon Tn1546, The two predominant types, Tn1546 and type 2 transposons, which differed at three band positions, were present in both human and animal isolates, Type 2 transposons were significantly associated with pig isolates. The other types were seldom detected. These data suggest a possible exchange of glycopeptide resistance markers between animals and humans.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENOMIC DNA, UNITED-STATES, VANCOMYCIN RESISTANCE, HOSPITALIZED-PATIENTS, MOLECULAR ANALYSIS, FARM-ANIMALS, COMMUNITY, SUSCEPTIBILITY, ANTIMICROBIAL RESISTANCE, FIELD GEL-ELECTROPHORESIS
journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Antimicrob. Agents Chemother.
volume
43
issue
8
pages
2032 - 2037
Web of Science type
Article
Web of Science id
000081789200034
ISSN
0066-4804
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
112920
handle
http://hdl.handle.net/1854/LU-112920
alternative location
http://aac.asm.org/cgi/content/abstract/43/8/2032
date created
2004-01-14 13:35:00
date last changed
2010-09-24 10:45:44
@article{112920,
  abstract     = {One hundred thirty-two glycopeptide-resistant Enterococcus faecium (GREF) isolates from different hospitals and pig and poultry farms in Belgium were compared on the basis of (i) their antibiotic susceptibilities, (ii) their SmaI pulsed-field gel electrophoresis (PFGE) patterns, and (iii) the organization of their Tn1546 or related elements in order to detect possible phenotypic and genotypic relationships among both groups of isolates. Human and animal vanA-positive GREF isolates were found to have similar susceptibility patterns; they remained susceptible to gentamicin and were, in general, susceptible to ampicillin. PFGE demonstrated a very high degree of genomic heterogeneity in both groups of isolates, However, indistinguishable isolates were found within different farms or hospitals, and in two instances, epidemiologically unrelated pig and human isolates showed indistinguishable PFGE patterns. In total, eight different transposon types were identified, and all were related to the prototype transposon Tn1546, The two predominant types, Tn1546 and type 2 transposons, which differed at three band positions, were present in both human and animal isolates, Type 2 transposons were significantly associated with pig isolates. The other types were seldom detected. These data suggest a possible exchange of glycopeptide resistance markers between animals and humans.},
  author       = {Descheemaeker, Patrick and Chapelle, Sabine and Devriese, Luc and Butaye, Patrick and Vandamme, Peter and Goossens, Herman},
  issn         = {0066-4804},
  journal      = {ANTIMICROBIAL AGENTS AND CHEMOTHERAPY},
  keyword      = {GENOMIC DNA,UNITED-STATES,VANCOMYCIN RESISTANCE,HOSPITALIZED-PATIENTS,MOLECULAR ANALYSIS,FARM-ANIMALS,COMMUNITY,SUSCEPTIBILITY,ANTIMICROBIAL RESISTANCE,FIELD GEL-ELECTROPHORESIS},
  language     = {eng},
  number       = {8},
  pages        = {2032--2037},
  title        = {Comparison of glycopeptide-resistant Enterococcus faecium isolates and glycopeptide resistance genes of human and animal origins},
  url          = {http://aac.asm.org/cgi/content/abstract/43/8/2032},
  volume       = {43},
  year         = {1999},
}

Chicago
Descheemaeker, Patrick, Sabine Chapelle, Luc Devriese, Patrick Butaye, Peter Vandamme, and Herman Goossens. 1999. “Comparison of Glycopeptide-resistant Enterococcus Faecium Isolates and Glycopeptide Resistance Genes of Human and Animal Origins.” Antimicrobial Agents and Chemotherapy 43 (8): 2032–2037.
APA
Descheemaeker, P., Chapelle, S., Devriese, L., Butaye, P., Vandamme, P., & Goossens, H. (1999). Comparison of glycopeptide-resistant Enterococcus faecium isolates and glycopeptide resistance genes of human and animal origins. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 43(8), 2032–2037.
Vancouver
1.
Descheemaeker P, Chapelle S, Devriese L, Butaye P, Vandamme P, Goossens H. Comparison of glycopeptide-resistant Enterococcus faecium isolates and glycopeptide resistance genes of human and animal origins. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 1999;43(8):2032–7.
MLA
Descheemaeker, Patrick, Sabine Chapelle, Luc Devriese, et al. “Comparison of Glycopeptide-resistant Enterococcus Faecium Isolates and Glycopeptide Resistance Genes of Human and Animal Origins.” ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 43.8 (1999): 2032–2037. Print.