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Molecular cloning of GAFP-1, an antifungal protein from Gastrodia elata

(1999) ACTA BOTANICA SINICA. 41(10). p.1041-1045
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Abstract
The cloning and sequencing of a full length cDNA of GAFP-1 ( Gastrodia antifungal protein), an antifungal protein from Gastrodia elata Bl. f, flavida S. Chow is reported. Degenerate primers were designed based on the N-terminal partial sequence from purified GAFP-1 to amplify the corresponding cDNA by rapid amplification of cDNA ends (RACE). A cDNA was obtained that contains an open reading frame for a peptide of 171 amino acids which matches the known peptide sequences.: A 5'UTR (untranslated region) of 55 bp was found upstream from the translation initiation site. Two poly(A) adenylation sites were located downstream the stop codon, GAFP-1 cDNA and its deduced amino acid sequence share high homology with the mannose binding lectins from Epipactis helloborine, Listera ovata and snowdrop (Galanthus nivalis). The cDNA can now be used for testing the potential of GAFP-1 for engineering fungal resistance in crop plants.
Keywords
cloning, rapid amplification of cDNA ends (RACE), LECTINS, MANNOSE, antifungal protein, Gastrodia eleta

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Citation

Please use this url to cite or link to this publication:

Chicago
Wang, Xiao-Chen, Wilson Ardiles Diaz, Guy Bauw, Qing Xu, Marc Van Montagu, Zhang-Liang Chen, and Willy Dillen. 1999. “Molecular Cloning of GAFP-1, an Antifungal Protein from Gastrodia Elata.” Acta Botanica Sinica 41 (10): 1041–1045.
APA
Wang, Xiao-Chen, Ardiles Diaz, W., Bauw, G., Xu, Q., Van Montagu, M., Chen, Z.-L., & Dillen, W. (1999). Molecular cloning of GAFP-1, an antifungal protein from Gastrodia elata. ACTA BOTANICA SINICA, 41(10), 1041–1045.
Vancouver
1.
Wang X-C, Ardiles Diaz W, Bauw G, Xu Q, Van Montagu M, Chen Z-L, et al. Molecular cloning of GAFP-1, an antifungal protein from Gastrodia elata. ACTA BOTANICA SINICA. 1999;41(10):1041–5.
MLA
Wang, Xiao-Chen, Wilson Ardiles Diaz, Guy Bauw, et al. “Molecular Cloning of GAFP-1, an Antifungal Protein from Gastrodia Elata.” ACTA BOTANICA SINICA 41.10 (1999): 1041–1045. Print.
@article{112821,
  abstract     = {The cloning and sequencing of a full length cDNA of GAFP-1 ( Gastrodia antifungal protein), an antifungal protein from Gastrodia elata Bl. f, flavida S. Chow is reported. Degenerate primers were designed based on the N-terminal partial sequence from purified GAFP-1 to amplify the corresponding cDNA by rapid amplification of cDNA ends (RACE). A cDNA was obtained that contains an open reading frame for a peptide of 171 amino acids which matches the known peptide sequences.: A 5'UTR (untranslated region) of 55 bp was found upstream from the translation initiation site. Two poly(A) adenylation sites were located downstream the stop codon, GAFP-1 cDNA and its deduced amino acid sequence share high homology with the mannose binding lectins from Epipactis helloborine, Listera ovata and snowdrop (Galanthus nivalis). The cDNA can now be used for testing the potential of GAFP-1 for engineering fungal resistance in crop plants.},
  author       = {Wang, Xiao-Chen and Ardiles Diaz, Wilson and Bauw, Guy and Xu, Qing and Van Montagu, Marc and Chen, Zhang-Liang and Dillen, Willy},
  issn         = {0577-7496},
  journal      = {ACTA BOTANICA SINICA},
  language     = {chi},
  number       = {10},
  pages        = {1041--1045},
  title        = {Molecular cloning of GAFP-1, an antifungal protein from Gastrodia elata},
  volume       = {41},
  year         = {1999},
}

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