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Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers

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Abstract
This study demonstrates the relative ease of generating high-density linkage maps using the AFLP(R) technology. Two high-density AFLP linkage maps of Zea mays L. were generated based on: (1) a B73 x Mo17 recombinant inbred population and (2) a D32 x D145 immortalized F-2 population. Although AFLP technology is in essence a mono-allelic marker system, markers can be scored quantitatively and used to deduce zygosity. AFLP markers were generated using the enzyme combinations EcoRI/MseI and PstI/MseI. A total of 1539 and 1355 AFLP markers have been mapped in the two populations, respectively. Among the mapped PstI/MseI AFLP markers we have included fragments bounded by a methylated PstI site ((m)AFLP markers). Mapping these (m)AFLP markers shows that the presence of C-methylation segregates in perfect accordance with the primary target sequence, leading to Mendelian inheritance. Simultaneous mapping of PstI/MseI AFLP and PstI/MseI (m)AFLP markers allowed us to identify a number of epi-alleles, showing allelic variation in the CpNpG methylation only. However, their frequency in maize is low. Map comparison shows that, despite some rearrangements, most of the AFLP markers that are common in both populations, map at similar positions, This would indicate that AFLP markers are predominantly single-locus markers. Changes in map order occur mainly in marker-dense regions. These marker-dense regions, representing clusters of mainly EcoRI/MseI AFLP and PstI/MseI (m)AFLP markers, colocalize well with the putative centromeric regions of the maize chromosomes. In contrast, PstI/MseI markers are more uniformly distributed over the genome.
Keywords
Zea mays L., AFLP (R), methylation AFLP (R), genetic map, DNA methylation, FRAGMENT LENGTH POLYMORPHISMS, GENETIC-RELATIONSHIPS, ARABIDOPSIS-THALIANA, ARBITRARY PRIMERS, MAIZE, BARLEY, RESISTANCE, DIVERSITY, POTATO, RFLP

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Citation

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MLA
Vuylsteke, Marnik, R Mank, R Antonise, et al. “Two High-density AFLP® Linkage Maps of Zea Mays L.: Analysis of Distribution of AFLP Markers.” THEORETICAL AND APPLIED GENETICS 99.6 (1999): 921–935. Print.
APA
Vuylsteke, Marnik, Mank, R., Antonise, R., Bastiaans, E., Senior, M., Stuber, C., Melchinger, A., et al. (1999). Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers. THEORETICAL AND APPLIED GENETICS, 99(6), 921–935.
Chicago author-date
Vuylsteke, Marnik, R Mank, R Antonise, E Bastiaans, ML Senior, CW Stuber, AE Melchinger, et al. 1999. “Two High-density AFLP® Linkage Maps of Zea Mays L.: Analysis of Distribution of AFLP Markers.” Theoretical and Applied Genetics 99 (6): 921–935.
Chicago author-date (all authors)
Vuylsteke, Marnik, R Mank, R Antonise, E Bastiaans, ML Senior, CW Stuber, AE Melchinger, T Lübberstedt, XC Xia, P Stam, Marc Zabeau, and Martin Kuiper. 1999. “Two High-density AFLP® Linkage Maps of Zea Mays L.: Analysis of Distribution of AFLP Markers.” Theoretical and Applied Genetics 99 (6): 921–935.
Vancouver
1.
Vuylsteke M, Mank R, Antonise R, Bastiaans E, Senior M, Stuber C, et al. Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers. THEORETICAL AND APPLIED GENETICS. 1999;99(6):921–35.
IEEE
[1]
M. Vuylsteke et al., “Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers,” THEORETICAL AND APPLIED GENETICS, vol. 99, no. 6, pp. 921–935, 1999.
@article{112810,
  abstract     = {This study demonstrates the relative ease of generating high-density linkage maps using the AFLP(R) technology. Two high-density AFLP linkage maps of Zea mays L. were generated based on: (1) a B73 x Mo17 recombinant inbred population and (2) a D32 x D145 immortalized F-2 population. Although AFLP technology is in essence a mono-allelic marker system, markers can be scored quantitatively and used to deduce zygosity. AFLP markers were generated using the enzyme combinations EcoRI/MseI and PstI/MseI. A total of 1539 and 1355 AFLP markers have been mapped in the two populations, respectively. Among the mapped PstI/MseI AFLP markers we have included fragments bounded by a methylated PstI site ((m)AFLP markers). Mapping these (m)AFLP markers shows that the presence of C-methylation segregates in perfect accordance with the primary target sequence, leading to Mendelian inheritance. Simultaneous mapping of PstI/MseI AFLP and PstI/MseI (m)AFLP markers allowed us to identify a number of epi-alleles, showing allelic variation in the CpNpG methylation only. However, their frequency in maize is low. Map comparison shows that, despite some rearrangements, most of the AFLP markers that are common in both populations, map at similar positions, This would indicate that AFLP markers are predominantly single-locus markers. Changes in map order occur mainly in marker-dense regions. These marker-dense regions, representing clusters of mainly EcoRI/MseI AFLP and PstI/MseI (m)AFLP markers, colocalize well with the putative centromeric regions of the maize chromosomes. In contrast, PstI/MseI markers are more uniformly distributed over the genome.},
  author       = {Vuylsteke, Marnik and Mank, R and Antonise, R and Bastiaans, E and Senior, ML and Stuber, CW and Melchinger, AE and Lübberstedt, T and Xia, XC and Stam, P and Zabeau, Marc and Kuiper, Martin},
  issn         = {0040-5752},
  journal      = {THEORETICAL AND APPLIED GENETICS},
  keywords     = {Zea mays L.,AFLP (R),methylation AFLP (R),genetic map,DNA methylation,FRAGMENT LENGTH POLYMORPHISMS,GENETIC-RELATIONSHIPS,ARABIDOPSIS-THALIANA,ARBITRARY PRIMERS,MAIZE,BARLEY,RESISTANCE,DIVERSITY,POTATO,RFLP},
  language     = {eng},
  number       = {6},
  pages        = {921--935},
  title        = {Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers},
  url          = {http://dx.doi.org/10.1007/s001220051399},
  volume       = {99},
  year         = {1999},
}

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