NaCl and CuSO₄ treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L.
- Author
- Arnould Savouré, Denise Thorin, Mark W Davey (UGent) , Xuejun Hua (UGent) , S Mauro, Marc Van Montagu (UGent) , Dirk Inzé (UGent) and Nathalie Verbruggen (UGent)
- Organization
- Abstract
- To gain better insights into the generation of oxidative stress by NaCl stress, we compared the effects of toxic, sublethal concentrations of NaCl (150 mM) with those of CuSO4 (100 mu M) On the antioxidant defence responses of Nicotiana plumbaginifolia L. plants grown in hydroponic culture. Short- and long-term adaptive responses were investigated as well as the effects of root cutting to allow rapid absorption of ions. Our results show that photosynthesis is already affected after 12 h of both treatments and that photoinhibition is induced. Furthermore, the two treatments showed distinct patterns of induction of the genes of the antioxidant systems. Principally, NaCl stimulated catalase activity through activation of the Cat2 and Call genes, whereas with CuSO4, transcript levels, protein levels, and enzymatic activities of glutathione peroxidase, ascorbate peroxidase, and superoxide dismutase were differentially induced. The antioxidants ascorbate and glutathione were found to be largely oxidized after 12 h, suggesting an inability of the recycling process to cope with the stress, In addition, both NaCl and CuSO4 treatments stimulated the accumulation of the osmolyte proline, Therefore, we conclude that the uptake and accumulation of different types of ions lead to the generation of distinct antioxidant responses in N. plumbaginifolia.
- Keywords
- COPPER, salt stress, GLUTATHIONE PEROXIDASES, Nicotiana plumbaginifolia L., ascorbate-glutathione cycle, oxidative stress, copper stress, SUPEROXIDE-DISMUTASE, CYTOSOLIC ASCORBATE PEROXIDASE, SALT-STRESS, ANTIOXIDANT ENZYMES, LOW-TEMPERATURE, OXYGEN RADICALS, CATALASE, PLANTS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-112738
- MLA
- Savouré, Arnould, et al. “NaCl and CuSO₄ Treatments Trigger Distinct Oxidative Defence Mechanisms in Nicotiana Plumbaginifolia L.” PLANT CELL AND ENVIRONMENT, vol. 22, no. 4, 1999, pp. 387–96, doi:10.1046/j.1365-3040.1999.00404.x.
- APA
- Savouré, A., Thorin, D., Davey, M. W., Hua, X., Mauro, S., Van Montagu, M., … Verbruggen, N. (1999). NaCl and CuSO₄ treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L. PLANT CELL AND ENVIRONMENT, 22(4), 387–396. https://doi.org/10.1046/j.1365-3040.1999.00404.x
- Chicago author-date
- Savouré, Arnould, Denise Thorin, Mark W Davey, Xuejun Hua, S Mauro, Marc Van Montagu, Dirk Inzé, and Nathalie Verbruggen. 1999. “NaCl and CuSO₄ Treatments Trigger Distinct Oxidative Defence Mechanisms in Nicotiana Plumbaginifolia L.” PLANT CELL AND ENVIRONMENT 22 (4): 387–96. https://doi.org/10.1046/j.1365-3040.1999.00404.x.
- Chicago author-date (all authors)
- Savouré, Arnould, Denise Thorin, Mark W Davey, Xuejun Hua, S Mauro, Marc Van Montagu, Dirk Inzé, and Nathalie Verbruggen. 1999. “NaCl and CuSO₄ Treatments Trigger Distinct Oxidative Defence Mechanisms in Nicotiana Plumbaginifolia L.” PLANT CELL AND ENVIRONMENT 22 (4): 387–396. doi:10.1046/j.1365-3040.1999.00404.x.
- Vancouver
- 1.Savouré A, Thorin D, Davey MW, Hua X, Mauro S, Van Montagu M, et al. NaCl and CuSO₄ treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L. PLANT CELL AND ENVIRONMENT. 1999;22(4):387–96.
- IEEE
- [1]A. Savouré et al., “NaCl and CuSO₄ treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L.,” PLANT CELL AND ENVIRONMENT, vol. 22, no. 4, pp. 387–396, 1999.
@article{112738,
abstract = {{To gain better insights into the generation of oxidative stress by NaCl stress, we compared the effects of toxic, sublethal concentrations of NaCl (150 mM) with those of CuSO4 (100 mu M) On the antioxidant defence responses of Nicotiana plumbaginifolia L. plants grown in hydroponic culture. Short- and long-term adaptive responses were investigated as well as the effects of root cutting to allow rapid absorption of ions. Our results show that photosynthesis is already affected after 12 h of both treatments and that photoinhibition is induced. Furthermore, the two treatments showed distinct patterns of induction of the genes of the antioxidant systems. Principally, NaCl stimulated catalase activity through activation of the Cat2 and Call genes, whereas with CuSO4, transcript levels, protein levels, and enzymatic activities of glutathione peroxidase, ascorbate peroxidase, and superoxide dismutase were differentially induced. The antioxidants ascorbate and glutathione were found to be largely oxidized after 12 h, suggesting an inability of the recycling process to cope with the stress, In addition, both NaCl and CuSO4 treatments stimulated the accumulation of the osmolyte proline, Therefore, we conclude that the uptake and accumulation of different types of ions lead to the generation of distinct antioxidant responses in N. plumbaginifolia.}},
author = {{Savouré, Arnould and Thorin, Denise and Davey, Mark W and Hua, Xuejun and Mauro, S and Van Montagu, Marc and Inzé, Dirk and Verbruggen, Nathalie}},
issn = {{0140-7791}},
journal = {{PLANT CELL AND ENVIRONMENT}},
keywords = {{COPPER,salt stress,GLUTATHIONE PEROXIDASES,Nicotiana plumbaginifolia L.,ascorbate-glutathione cycle,oxidative stress,copper stress,SUPEROXIDE-DISMUTASE,CYTOSOLIC ASCORBATE PEROXIDASE,SALT-STRESS,ANTIOXIDANT ENZYMES,LOW-TEMPERATURE,OXYGEN RADICALS,CATALASE,PLANTS}},
language = {{eng}},
number = {{4}},
pages = {{387--396}},
title = {{NaCl and CuSO₄ treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L.}},
url = {{http://dx.doi.org/10.1046/j.1365-3040.1999.00404.x}},
volume = {{22}},
year = {{1999}},
}
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