Advanced search
1 file | 3.95 MB

carpel, a new Arabidopsis epi-mutant of the SUPERMAN gene: phenotypic analysis and DNA methylation status

(1999) PLANT AND CELL PHYSIOLOGY. 40(9). p.961-972
Author
Organization
Abstract
The carpel (car) mutation affects the morphology of reproductive organs in Arabidopsis thaliana, car flowers have an increased number of carpels, on average 2.7+/-0.8 instead of two in the wild type. Through allelism test with fon1-3 and analysis of the methylation state of the SUPERMAN (SUP) gene in car mutants, we show that car is an epi-mutation of SUP. The methylation pattern of car is clearly distinct from that of fon1-3, another epimutation of the SUP gene. Methylation was found predominantly in Cp(A/T)p(A/G) triplets and in CpG pairs. We suggest that the extensive SUP methylation in car has arisen from an abundant methylation of a single CpG site that was already present in abscisic acid-insensitive (abi3-4) mutants, from which car was segregating.
Keywords
epi-mutant, DNA methylation, SUPERMAN, FLOWER DEVELOPMENT, CYTOSINE METHYLATION, HOMEOTIC GENES, SEQUENCES, INITIATION, PATTERNS, METHYLTRANSFERASE, TRANSCRIPTION, MAINTENANCE, CONSEQUENCE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.95 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Rohde, Antje, Christoph Grunau, Lode De Beck, Marc Van Montagu, André Rosenthal, and Wout Boerjan. 1999. “Carpel, a New Arabidopsis Epi-mutant of the SUPERMAN Gene: Phenotypic Analysis and DNA Methylation Status.” Plant and Cell Physiology 40 (9): 961–972.
APA
Rohde, A., Grunau, C., De Beck, L., Van Montagu, M., Rosenthal, A., & Boerjan, W. (1999). carpel, a new Arabidopsis epi-mutant of the SUPERMAN gene: phenotypic analysis and DNA methylation status. PLANT AND CELL PHYSIOLOGY, 40(9), 961–972.
Vancouver
1.
Rohde A, Grunau C, De Beck L, Van Montagu M, Rosenthal A, Boerjan W. carpel, a new Arabidopsis epi-mutant of the SUPERMAN gene: phenotypic analysis and DNA methylation status. PLANT AND CELL PHYSIOLOGY. 1999;40(9):961–72.
MLA
Rohde, Antje, Christoph Grunau, Lode De Beck, et al. “Carpel, a New Arabidopsis Epi-mutant of the SUPERMAN Gene: Phenotypic Analysis and DNA Methylation Status.” PLANT AND CELL PHYSIOLOGY 40.9 (1999): 961–972. Print.
@article{112728,
  abstract     = {The carpel (car) mutation affects the morphology of reproductive organs in Arabidopsis thaliana, car flowers have an increased number of carpels, on average 2.7+/-0.8 instead of two in the wild type. Through allelism test with fon1-3 and analysis of the methylation state of the SUPERMAN (SUP) gene in car mutants, we show that car is an epi-mutation of SUP. The methylation pattern of car is clearly distinct from that of fon1-3, another epimutation of the SUP gene. Methylation was found predominantly in Cp(A/T)p(A/G) triplets and in CpG pairs. We suggest that the extensive SUP methylation in car has arisen from an abundant methylation of a single CpG site that was already present in abscisic acid-insensitive (abi3-4) mutants, from which car was segregating.},
  author       = {Rohde, Antje and Grunau, Christoph and De Beck, Lode and Van Montagu, Marc and Rosenthal, Andr{\'e} and Boerjan, Wout},
  issn         = {0032-0781},
  journal      = {PLANT AND CELL PHYSIOLOGY},
  keyword      = {epi-mutant,DNA methylation,SUPERMAN,FLOWER DEVELOPMENT,CYTOSINE METHYLATION,HOMEOTIC GENES,SEQUENCES,INITIATION,PATTERNS,METHYLTRANSFERASE,TRANSCRIPTION,MAINTENANCE,CONSEQUENCE},
  language     = {eng},
  number       = {9},
  pages        = {961--972},
  title        = {carpel, a new Arabidopsis epi-mutant of the SUPERMAN gene: phenotypic analysis and DNA methylation status},
  url          = {http://pcp.oxfordjournals.org/content/40/9/961.abstract},
  volume       = {40},
  year         = {1999},
}

Web of Science
Times cited: