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The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants

Alain Goossens (UGent) , Willy Dillen (UGent) , Janniek De Clercq (UGent) , Marc Van Montagu (UGent) and Geert Angenon (UGent)
(1999) PLANT PHYSIOLOGY. 120(4). p.1095-1103
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Abstract
The regulatory sequences of many genes encoding seed storage proteins have been used to drive seed-specific expression of a variety of proteins in transgenic plants. Because the levels at which these transgene-derived proteins accumulate are generally quite low, we investigated the utility of the arcelin-5 regulatory sequences in obtaining high seed-specific expression in transgenic plants. Arcelin-5 is an abundant seed protein found in some wild common bean (Phaseolus vulgaris L.) genotypes. Seeds of Arabidopsis and Tepary bean (Phaseolus acutifolius A. Gray) plants transformed with arcelin-5 gene constructs synthesized arcelin-5 to levels of 15% and 25% of the total protein content, respectively. To our knowledge, such high expression levels directed by a transgene have not been reported before. The transgenic plants also showed low plant-to-plant variation in arcelin expression. Complex transgene integration patterns, which often result in gene silencing effects, were not associated with reduced arcelin-5 expression. High transgene expression was the result of high mRNA steady-state levels and was restricted to seeds. This indicates that all requirements for high seed-specific expression are cis elements present in the cloned genomic arcelin-5 sequence and trans-acting factors that are available in Arabidopsis and Phaseolus spp., and thus probably in most dicotyledonous plants.
Keywords
STORAGE PROTEIN GENE, MEDIATED TRANSFORMATION, 2S ALBUMIN, TOBACCO SEEDS, PETUNIA PLANTS, ATTACHMENT REGIONS, TISSUE-SPECIFIC EXPRESSION, RICE ENDOSPERM, BETA-PHASEOLIN, BERTHOLLETIA-EXCELSA

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MLA
Goossens, Alain, Willy Dillen, Janniek De Clercq, et al. “The Arcelin-5 Gene of Phaseolus Vulgaris Directs High Seed-specific Expression in Transgenic Phaseolus Acutifolius and Arabidopsis Plants.” PLANT PHYSIOLOGY 120.4 (1999): 1095–1103. Print.
APA
Goossens, Alain, Dillen, W., De Clercq, J., Van Montagu, M., & Angenon, G. (1999). The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants. PLANT PHYSIOLOGY, 120(4), 1095–1103.
Chicago author-date
Goossens, Alain, Willy Dillen, Janniek De Clercq, Marc Van Montagu, and Geert Angenon. 1999. “The Arcelin-5 Gene of Phaseolus Vulgaris Directs High Seed-specific Expression in Transgenic Phaseolus Acutifolius and Arabidopsis Plants.” Plant Physiology 120 (4): 1095–1103.
Chicago author-date (all authors)
Goossens, Alain, Willy Dillen, Janniek De Clercq, Marc Van Montagu, and Geert Angenon. 1999. “The Arcelin-5 Gene of Phaseolus Vulgaris Directs High Seed-specific Expression in Transgenic Phaseolus Acutifolius and Arabidopsis Plants.” Plant Physiology 120 (4): 1095–1103.
Vancouver
1.
Goossens A, Dillen W, De Clercq J, Van Montagu M, Angenon G. The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants. PLANT PHYSIOLOGY. 1999;120(4):1095–103.
IEEE
[1]
A. Goossens, W. Dillen, J. De Clercq, M. Van Montagu, and G. Angenon, “The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants,” PLANT PHYSIOLOGY, vol. 120, no. 4, pp. 1095–1103, 1999.
@article{112651,
  abstract     = {The regulatory sequences of many genes encoding seed storage proteins have been used to drive seed-specific expression of a variety of proteins in transgenic plants. Because the levels at which these transgene-derived proteins accumulate are generally quite low, we investigated the utility of the arcelin-5 regulatory sequences in obtaining high seed-specific expression in transgenic plants. Arcelin-5 is an abundant seed protein found in some wild common bean (Phaseolus vulgaris L.) genotypes. Seeds of Arabidopsis and Tepary bean (Phaseolus acutifolius A. Gray) plants transformed with arcelin-5 gene constructs synthesized arcelin-5 to levels of 15% and 25% of the total protein content, respectively. To our knowledge, such high expression levels directed by a transgene have not been reported before. The transgenic plants also showed low plant-to-plant variation in arcelin expression. Complex transgene integration patterns, which often result in gene silencing effects, were not associated with reduced arcelin-5 expression. High transgene expression was the result of high mRNA steady-state levels and was restricted to seeds. This indicates that all requirements for high seed-specific expression are cis elements present in the cloned genomic arcelin-5 sequence and trans-acting factors that are available in Arabidopsis and Phaseolus spp., and thus probably in most dicotyledonous plants.},
  author       = {Goossens, Alain and Dillen, Willy and De Clercq, Janniek and Van Montagu, Marc and Angenon, Geert},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keywords     = {STORAGE PROTEIN GENE,MEDIATED TRANSFORMATION,2S ALBUMIN,TOBACCO SEEDS,PETUNIA PLANTS,ATTACHMENT REGIONS,TISSUE-SPECIFIC EXPRESSION,RICE ENDOSPERM,BETA-PHASEOLIN,BERTHOLLETIA-EXCELSA},
  language     = {eng},
  number       = {4},
  pages        = {1095--1103},
  title        = {The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants},
  url          = {http://dx.doi.org/10.1104/pp.120.4.1095},
  volume       = {120},
  year         = {1999},
}

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