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Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray

Willy Dillen (UGent) , Janniek De Clercq (UGent) , Alain Goossens (UGent) , Marc Van Montagu (UGent) and Geert Angenon (UGent)
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Abstract
Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray has been achieved. Regeneration-competent callus, obtained from bud explants of greenhouse-grown plants, was co-cultivated with Agrobacterium tumefaciens C58C1Rif(R)(pMP90) harbouring a binary vector with the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) marker genes. Transient expression of uidA was detected in five out of six genotypes tested. Transgenic callus lines of three genotypes were established on geneticin-containing medium. Plants were recovered from one line (genotype NI 576). This line had been transformed with a binary plasmid which, in addition to the marker genes, contained a genomic fragment encoding the Phaseolus vulgaris arcelin-5a protein. This seed storage protein presumably confers resistance to the insect Zabrotes subfasciatus, a major pest of P. vulgaris. Integration of foreign DNA was confirmed by molecular analysis. The introduced genes segregated as a single locus. Arcelin-5a was produced at high levels in seeds. The possibility of using P. acutifolius as a 'bridging' species to introduce transgenes into the economically more important species P. vulgaris is discussed.
Keywords
2S ALBUMIN GENE, SOYBEAN GLYCINE-MAX, PISUM-SATIVUM L, VULGARIS L, STABLE TRANSFORMATION, PLANT-REGENERATION, PARTICLE-ACCELERATION, SHOOT ORGANOGENESIS, NUT BERTHOLLETIA-EXCELSA, TRANSGENIC VICIA-NARBONENSIS, tepary bean, insect resistance, genetic transformation, arcelin, Fabaceae

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Chicago
Dillen, Willy, Janniek De Clercq, Alain Goossens, Marc Van Montagu, and Geert Angenon. 1997. “Agrobacterium-mediated Transformation of Phaseolus Acutifolius A. Gray.” Theoretical and Applied Genetics 94 (2): 151–158.
APA
Dillen, W., De Clercq, J., Goossens, A., Van Montagu, M., & Angenon, G. (1997). Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray. THEORETICAL AND APPLIED GENETICS, 94(2), 151–158.
Vancouver
1.
Dillen W, De Clercq J, Goossens A, Van Montagu M, Angenon G. Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray. THEORETICAL AND APPLIED GENETICS. 1997;94(2):151–8.
MLA
Dillen, Willy, Janniek De Clercq, Alain Goossens, et al. “Agrobacterium-mediated Transformation of Phaseolus Acutifolius A. Gray.” THEORETICAL AND APPLIED GENETICS 94.2 (1997): 151–158. Print.
@article{112627,
  abstract     = {Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray has been achieved. Regeneration-competent callus, obtained from bud explants of greenhouse-grown plants, was co-cultivated with Agrobacterium tumefaciens C58C1Rif(R)(pMP90) harbouring a binary vector with the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) marker genes. Transient expression of uidA was detected in five out of six genotypes tested. Transgenic callus lines of three genotypes were established on geneticin-containing medium. Plants were recovered from one line (genotype NI 576). This line had been transformed with a binary plasmid which, in addition to the marker genes, contained a genomic fragment encoding the Phaseolus vulgaris arcelin-5a protein. This seed storage protein presumably confers resistance to the insect Zabrotes subfasciatus, a major pest of P. vulgaris. Integration of foreign DNA was confirmed by molecular analysis. The introduced genes segregated as a single locus. Arcelin-5a was produced at high levels in seeds. The possibility of using P. acutifolius as a 'bridging' species to introduce transgenes into the economically more important species P. vulgaris is discussed.},
  author       = {Dillen, Willy and De Clercq, Janniek and Goossens, Alain and Van Montagu, Marc and Angenon, Geert},
  issn         = {0040-5752},
  journal      = {THEORETICAL AND APPLIED GENETICS},
  keyword      = {2S ALBUMIN GENE,SOYBEAN GLYCINE-MAX,PISUM-SATIVUM L,VULGARIS L,STABLE TRANSFORMATION,PLANT-REGENERATION,PARTICLE-ACCELERATION,SHOOT ORGANOGENESIS,NUT BERTHOLLETIA-EXCELSA,TRANSGENIC VICIA-NARBONENSIS,tepary bean,insect resistance,genetic transformation,arcelin,Fabaceae},
  language     = {eng},
  number       = {2},
  pages        = {151--158},
  title        = {Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray},
  url          = {http://dx.doi.org/10.1007/s001220050394},
  volume       = {94},
  year         = {1997},
}

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