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Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray

Willy Dillen UGent, Janniek De Clercq UGent, Alain Goossens UGent, Marc Van Montagu UGent and Geert Angenon UGent (1997) THEORETICAL AND APPLIED GENETICS. 94(2). p.151-158
abstract
Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray has been achieved. Regeneration-competent callus, obtained from bud explants of greenhouse-grown plants, was co-cultivated with Agrobacterium tumefaciens C58C1Rif(R)(pMP90) harbouring a binary vector with the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) marker genes. Transient expression of uidA was detected in five out of six genotypes tested. Transgenic callus lines of three genotypes were established on geneticin-containing medium. Plants were recovered from one line (genotype NI 576). This line had been transformed with a binary plasmid which, in addition to the marker genes, contained a genomic fragment encoding the Phaseolus vulgaris arcelin-5a protein. This seed storage protein presumably confers resistance to the insect Zabrotes subfasciatus, a major pest of P. vulgaris. Integration of foreign DNA was confirmed by molecular analysis. The introduced genes segregated as a single locus. Arcelin-5a was produced at high levels in seeds. The possibility of using P. acutifolius as a 'bridging' species to introduce transgenes into the economically more important species P. vulgaris is discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
2S ALBUMIN GENE, SOYBEAN GLYCINE-MAX, PISUM-SATIVUM L, VULGARIS L, STABLE TRANSFORMATION, PLANT-REGENERATION, PARTICLE-ACCELERATION, SHOOT ORGANOGENESIS, NUT BERTHOLLETIA-EXCELSA, TRANSGENIC VICIA-NARBONENSIS, tepary bean, insect resistance, genetic transformation, arcelin, Fabaceae
journal title
THEORETICAL AND APPLIED GENETICS
Theor. Appl. Genet.
volume
94
issue
2
pages
151 - 158
Web of Science type
Article
ISSN
0040-5752
DOI
10.1007/s001220050394
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
112627
handle
http://hdl.handle.net/1854/LU-112627
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:01
@article{112627,
  abstract     = {Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray has been achieved. Regeneration-competent callus, obtained from bud explants of greenhouse-grown plants, was co-cultivated with Agrobacterium tumefaciens C58C1Rif(R)(pMP90) harbouring a binary vector with the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) marker genes. Transient expression of uidA was detected in five out of six genotypes tested. Transgenic callus lines of three genotypes were established on geneticin-containing medium. Plants were recovered from one line (genotype NI 576). This line had been transformed with a binary plasmid which, in addition to the marker genes, contained a genomic fragment encoding the Phaseolus vulgaris arcelin-5a protein. This seed storage protein presumably confers resistance to the insect Zabrotes subfasciatus, a major pest of P. vulgaris. Integration of foreign DNA was confirmed by molecular analysis. The introduced genes segregated as a single locus. Arcelin-5a was produced at high levels in seeds. The possibility of using P. acutifolius as a 'bridging' species to introduce transgenes into the economically more important species P. vulgaris is discussed.},
  author       = {Dillen, Willy and De Clercq, Janniek and Goossens, Alain and Van Montagu, Marc and Angenon, Geert},
  issn         = {0040-5752},
  journal      = {THEORETICAL AND APPLIED GENETICS},
  keyword      = {2S ALBUMIN GENE,SOYBEAN GLYCINE-MAX,PISUM-SATIVUM L,VULGARIS L,STABLE TRANSFORMATION,PLANT-REGENERATION,PARTICLE-ACCELERATION,SHOOT ORGANOGENESIS,NUT BERTHOLLETIA-EXCELSA,TRANSGENIC VICIA-NARBONENSIS,tepary bean,insect resistance,genetic transformation,arcelin,Fabaceae},
  language     = {eng},
  number       = {2},
  pages        = {151--158},
  title        = {Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray},
  url          = {http://dx.doi.org/10.1007/s001220050394},
  volume       = {94},
  year         = {1997},
}

Chicago
Dillen, Willy, Janniek De Clercq, Alain Goossens, Marc Van Montagu, and Geert Angenon. 1997. “Agrobacterium-mediated Transformation of Phaseolus Acutifolius A. Gray.” Theoretical and Applied Genetics 94 (2): 151–158.
APA
Dillen, W., De Clercq, J., Goossens, A., Van Montagu, M., & Angenon, G. (1997). Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray. THEORETICAL AND APPLIED GENETICS, 94(2), 151–158.
Vancouver
1.
Dillen W, De Clercq J, Goossens A, Van Montagu M, Angenon G. Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray. THEORETICAL AND APPLIED GENETICS. 1997;94(2):151–8.
MLA
Dillen, Willy, Janniek De Clercq, Alain Goossens, et al. “Agrobacterium-mediated Transformation of Phaseolus Acutifolius A. Gray.” THEORETICAL AND APPLIED GENETICS 94.2 (1997): 151–158. Print.