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Gene silencing results in instability of antibody production in transgenic plants

Myriam De Neve (UGent) , Sylvie De Buck (UGent) , Chris De Wilde (UGent) , Helena Van Houdt (UGent) , I Strobbe, Anni Jacobs (UGent) , Marc Van Montagu (UGent) and Anna Depicker (UGent)
(1999) MOLECULAR AND GENERAL GENETICS. 260(6). p.582-592
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Abstract
The stability of antibody and F-ab expression was assessed in five different homozygous transgenic Arabidopsis lines. Each of these lines showed silencing of the transgenes that: encode the antibody polypeptides, leading to instability of antibody production. However, each line had a different and specific instability profile. The characteristic variation in the level of antibody accumulation in each line as a function of developmental stage indicated that the T-DNA integration pattern played a role in triggering silencing, and also that the history and the integration position of simple transgene loci can influence the susceptibility to epigenetic silencing. In different lines with low antibody accumulation levels, methylation was found either in the promoter alone, in both the promoter and the transcribed region, in the transcribed region only, or in the transcribed region and downstream sequences. In conclusion, our data suggest that epigenetic effects result in different transgene expression profiles in each of the five Arabidopsis lines analyzed.
Keywords
transgene expression, instability, gene silencing, antibody production, DNA methylation, LEVEL, LOCI, INACTIVATION, EXPRESSION, ACCUMULATION, PETUNIA, ARABIDOPSIS, TOBACCO, CHALCONE SYNTHASE, DNA-METHYLATION

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Chicago
De Neve, Myriam, Sylvie De Buck, Chris De Wilde, Helena Van Houdt, I Strobbe, Anni Jacobs, Marc Van Montagu, and Anna Depicker. 1999. “Gene Silencing Results in Instability of Antibody Production in Transgenic Plants.” Molecular and General Genetics 260 (6): 582–592.
APA
De Neve, Myriam, De Buck, S., De Wilde, C., Van Houdt, H., Strobbe, I., Jacobs, A., Van Montagu, M., et al. (1999). Gene silencing results in instability of antibody production in transgenic plants. MOLECULAR AND GENERAL GENETICS, 260(6), 582–592.
Vancouver
1.
De Neve M, De Buck S, De Wilde C, Van Houdt H, Strobbe I, Jacobs A, et al. Gene silencing results in instability of antibody production in transgenic plants. MOLECULAR AND GENERAL GENETICS. 1999;260(6):582–92.
MLA
De Neve, Myriam, Sylvie De Buck, Chris De Wilde, et al. “Gene Silencing Results in Instability of Antibody Production in Transgenic Plants.” MOLECULAR AND GENERAL GENETICS 260.6 (1999): 582–592. Print.
@article{112610,
  abstract     = {The stability of antibody and F-ab expression was assessed in five different homozygous transgenic Arabidopsis lines. Each of these lines showed silencing of the transgenes that: encode the antibody polypeptides, leading to instability of antibody production. However, each line had a different and specific instability profile. The characteristic variation in the level of antibody accumulation in each line as a function of developmental stage indicated that the T-DNA integration pattern played a role in triggering silencing, and also that the history and the integration position of simple transgene loci can influence the susceptibility to epigenetic silencing. In different lines with low antibody accumulation levels, methylation was found either in the promoter alone, in both the promoter and the transcribed region, in the transcribed region only, or in the transcribed region and downstream sequences. In conclusion, our data suggest that epigenetic effects result in different transgene expression profiles in each of the five Arabidopsis lines analyzed.},
  author       = {De Neve, Myriam and De Buck, Sylvie and De Wilde, Chris and Van Houdt, Helena and Strobbe, I and Jacobs, Anni and Van Montagu, Marc and Depicker, Anna},
  issn         = {0026-8925},
  journal      = {MOLECULAR AND GENERAL GENETICS},
  keyword      = {transgene expression,instability,gene silencing,antibody production,DNA methylation,LEVEL,LOCI,INACTIVATION,EXPRESSION,ACCUMULATION,PETUNIA,ARABIDOPSIS,TOBACCO,CHALCONE SYNTHASE,DNA-METHYLATION},
  language     = {eng},
  number       = {6},
  pages        = {582--592},
  title        = {Gene silencing results in instability of antibody production in transgenic plants},
  url          = {http://dx.doi.org/10.1007/s004380050932},
  volume       = {260},
  year         = {1999},
}

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