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The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration

Sylvie De Buck (UGent) , Anni Jacobs (UGent) , Marc Van Montagu (UGent) and Anna Depicker (UGent)
(1999) PLANT JOURNAL. 20(3). p.295-304
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Abstract
After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyzed by PCR, Arabidopsis root cells were co-transformed with two different T-DNAs with distinct sequences adjacent to the T-DNA borders. Nine direct or inverted T-DNA border junctions were analyzed at the sequence level. Precise end-to-end fusions were found between two right border ends, whereas imprecise fusions and filler DNA were present in T-DNA linkages containing a left border end. The results suggest that end-to-end ligation of double-stranded T-DNAs occurs especially between right T-DNA ends and that illegitimate recombination on the basis of microhomology, deletions, repair activities and insertions of filler DNA is involved in the formation of left border T-DNA junctions. Therefore, a similar illegitimate recombination mechanism is proposed that is involved in the formation of complex T-DNA inserts as well as in the integration of the T-DNA in the plant genome.
Keywords
GENOME, PROTEIN, GENE, STRANDED-DNA, MEDIATED TRANSFORMATION, ILLEGITIMATE RECOMBINATION, PLANT-CELLS, AGROBACTERIUM-TUMEFACIENS VIRD2, CONJUGATION, ARABIDOPSIS

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Chicago
De Buck, Sylvie, Anni Jacobs, Marc Van Montagu, and Anna Depicker. 1999. “The DNA Sequences of T-DNA Junctions Suggest That Complex T-DNA Loci Are Formed by a Recombination Process Resembling T-DNA Integration.” Plant Journal 20 (3): 295–304.
APA
De Buck, S., Jacobs, A., Van Montagu, M., & Depicker, A. (1999). The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. PLANT JOURNAL, 20(3), 295–304.
Vancouver
1.
De Buck S, Jacobs A, Van Montagu M, Depicker A. The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. PLANT JOURNAL. 1999;20(3):295–304.
MLA
De Buck, Sylvie, Anni Jacobs, Marc Van Montagu, et al. “The DNA Sequences of T-DNA Junctions Suggest That Complex T-DNA Loci Are Formed by a Recombination Process Resembling T-DNA Integration.” PLANT JOURNAL 20.3 (1999): 295–304. Print.
@article{112589,
  abstract     = {After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyzed by PCR, Arabidopsis root cells were co-transformed with two different T-DNAs with distinct sequences adjacent to the T-DNA borders. Nine direct or inverted T-DNA border junctions were analyzed at the sequence level. Precise end-to-end fusions were found between two right border ends, whereas imprecise fusions and filler DNA were present in T-DNA linkages containing a left border end. The results suggest that end-to-end ligation of double-stranded T-DNAs occurs especially between right T-DNA ends and that illegitimate recombination on the basis of microhomology, deletions, repair activities and insertions of filler DNA is involved in the formation of left border T-DNA junctions. Therefore, a similar illegitimate recombination mechanism is proposed that is involved in the formation of complex T-DNA inserts as well as in the integration of the T-DNA in the plant genome.},
  author       = {De Buck, Sylvie and Jacobs, Anni and Van Montagu, Marc and Depicker, Anna},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {GENOME,PROTEIN,GENE,STRANDED-DNA,MEDIATED TRANSFORMATION,ILLEGITIMATE RECOMBINATION,PLANT-CELLS,AGROBACTERIUM-TUMEFACIENS VIRD2,CONJUGATION,ARABIDOPSIS},
  language     = {eng},
  number       = {3},
  pages        = {295--304},
  title        = {The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1999.00602.x},
  volume       = {20},
  year         = {1999},
}

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