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The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration

Sylvie De Buck UGent, Anni Jacobs UGent, Marc Van Montagu UGent and Anna Depicker UGent (1999) PLANT JOURNAL. 20(3). p.295-304
abstract
After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyzed by PCR, Arabidopsis root cells were co-transformed with two different T-DNAs with distinct sequences adjacent to the T-DNA borders. Nine direct or inverted T-DNA border junctions were analyzed at the sequence level. Precise end-to-end fusions were found between two right border ends, whereas imprecise fusions and filler DNA were present in T-DNA linkages containing a left border end. The results suggest that end-to-end ligation of double-stranded T-DNAs occurs especially between right T-DNA ends and that illegitimate recombination on the basis of microhomology, deletions, repair activities and insertions of filler DNA is involved in the formation of left border T-DNA junctions. Therefore, a similar illegitimate recombination mechanism is proposed that is involved in the formation of complex T-DNA inserts as well as in the integration of the T-DNA in the plant genome.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENOME, PROTEIN, GENE, STRANDED-DNA, MEDIATED TRANSFORMATION, ILLEGITIMATE RECOMBINATION, PLANT-CELLS, AGROBACTERIUM-TUMEFACIENS VIRD2, CONJUGATION, ARABIDOPSIS
journal title
PLANT JOURNAL
Plant J.
volume
20
issue
3
pages
295 - 304
Web of Science type
Article
Web of Science id
000084156700004
ISSN
0960-7412
DOI
10.1046/j.1365-313X.1999.00602.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
112589
handle
http://hdl.handle.net/1854/LU-112589
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:36:49
@article{112589,
  abstract     = {After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyzed by PCR, Arabidopsis root cells were co-transformed with two different T-DNAs with distinct sequences adjacent to the T-DNA borders. Nine direct or inverted T-DNA border junctions were analyzed at the sequence level. Precise end-to-end fusions were found between two right border ends, whereas imprecise fusions and filler DNA were present in T-DNA linkages containing a left border end. The results suggest that end-to-end ligation of double-stranded T-DNAs occurs especially between right T-DNA ends and that illegitimate recombination on the basis of microhomology, deletions, repair activities and insertions of filler DNA is involved in the formation of left border T-DNA junctions. Therefore, a similar illegitimate recombination mechanism is proposed that is involved in the formation of complex T-DNA inserts as well as in the integration of the T-DNA in the plant genome.},
  author       = {De Buck, Sylvie and Jacobs, Anni and Van Montagu, Marc and Depicker, Anna},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {GENOME,PROTEIN,GENE,STRANDED-DNA,MEDIATED TRANSFORMATION,ILLEGITIMATE RECOMBINATION,PLANT-CELLS,AGROBACTERIUM-TUMEFACIENS VIRD2,CONJUGATION,ARABIDOPSIS},
  language     = {eng},
  number       = {3},
  pages        = {295--304},
  title        = {The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1999.00602.x},
  volume       = {20},
  year         = {1999},
}

Chicago
De Buck, Sylvie, Anni Jacobs, Marc Van Montagu, and Anna Depicker. 1999. “The DNA Sequences of T-DNA Junctions Suggest That Complex T-DNA Loci Are Formed by a Recombination Process Resembling T-DNA Integration.” Plant Journal 20 (3): 295–304.
APA
De Buck, S., Jacobs, A., Van Montagu, M., & Depicker, A. (1999). The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. PLANT JOURNAL, 20(3), 295–304.
Vancouver
1.
De Buck S, Jacobs A, Van Montagu M, Depicker A. The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. PLANT JOURNAL. 1999;20(3):295–304.
MLA
De Buck, Sylvie, Anni Jacobs, Marc Van Montagu, et al. “The DNA Sequences of T-DNA Junctions Suggest That Complex T-DNA Loci Are Formed by a Recombination Process Resembling T-DNA Integration.” PLANT JOURNAL 20.3 (1999): 295–304. Print.