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Molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia

Janice de Almeida Engler UGent, Vera De Vleesschauwer UGent, Sylvia Burssens UGent, John L, Jr Celenza, Dirk Inzé UGent, Marc Van Montagu UGent, Gilbert Engler UGent and Godelieve Gheysen UGent (1999) PLANT CELL. 11(5). p.793-807
abstract
Root knot and cyst nematodes induce large multinucleated cells, designated giant cells and syncytia, respectively, in plant roots. We have used molecular markers to study cell cycle progression in these specialized feeding cells. In situ hybridization with two cyclin-dependent kinases and two cyclins showed that these genes were induced very early in galls and syncytia and that the feeding cells progressed through the G(2) phase. By using cell cycle blockers, DNA synthesis and progression through the G(2) phase, or mitosis, were shown to be essential for gall and syncytium establishment. When mitosis was blocked, further gall development was arrested, This result demonstrates that cycles of endoreduplication or other methods of DNA amplification are insufficient to drive giant cell expansion. On the other hand, syncytium development was much less affected by a mitotic block; however, syncytium expansion was inhibited.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ORGANIZATION, EXPRESSION, MICROTUBULE POLYMERIZATION INVITRO, ARABIDOPSIS-THALIANA ROOT, PLANT-PARASITIC NEMATODES, MELOIDOGYNE-INCOGNITA, DINITROANILINE HERBICIDE, ENDOSPERM DEVELOPMENT, PROTEIN-KINASE, GIANT-CELLS
journal title
PLANT CELL
Plant Cell
volume
11
issue
5
pages
793 - 807
Web of Science type
Article
Web of Science id
000080680200005
ISSN
1040-4651
DOI
10.1105/tpc.11.5.793
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
112584
handle
http://hdl.handle.net/1854/LU-112584
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:36:49
@article{112584,
  abstract     = {Root knot and cyst nematodes induce large multinucleated cells, designated giant cells and syncytia, respectively, in plant roots. We have used molecular markers to study cell cycle progression in these specialized feeding cells. In situ hybridization with two cyclin-dependent kinases and two cyclins showed that these genes were induced very early in galls and syncytia and that the feeding cells progressed through the G(2) phase. By using cell cycle blockers, DNA synthesis and progression through the G(2) phase, or mitosis, were shown to be essential for gall and syncytium establishment. When mitosis was blocked, further gall development was arrested, This result demonstrates that cycles of endoreduplication or other methods of DNA amplification are insufficient to drive giant cell expansion. On the other hand, syncytium development was much less affected by a mitotic block; however, syncytium expansion was inhibited.},
  author       = {de Almeida Engler, Janice and De Vleesschauwer, Vera and Burssens, Sylvia and Celenza, John L, Jr and Inz{\'e}, Dirk and Van Montagu, Marc and Engler, Gilbert and Gheysen, Godelieve},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {ORGANIZATION,EXPRESSION,MICROTUBULE POLYMERIZATION INVITRO,ARABIDOPSIS-THALIANA ROOT,PLANT-PARASITIC NEMATODES,MELOIDOGYNE-INCOGNITA,DINITROANILINE HERBICIDE,ENDOSPERM DEVELOPMENT,PROTEIN-KINASE,GIANT-CELLS},
  language     = {eng},
  number       = {5},
  pages        = {793--807},
  title        = {Molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia},
  url          = {http://dx.doi.org/10.1105/tpc.11.5.793},
  volume       = {11},
  year         = {1999},
}

Chicago
de Almeida Engler, Janice, Vera De Vleesschauwer, Sylvia Burssens, John L Celenza Jr, Dirk Inzé, Marc Van Montagu, Gilbert Engler, and Godelieve Gheysen. 1999. “Molecular Markers and Cell Cycle Inhibitors Show the Importance of Cell Cycle Progression in Nematode-induced Galls and Syncytia.” Plant Cell 11 (5): 793–807.
APA
de Almeida Engler, J., De Vleesschauwer, V., Burssens, S., Celenza, J. L., Jr, Inzé, D., Van Montagu, M., Engler, G., et al. (1999). Molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia. PLANT CELL, 11(5), 793–807.
Vancouver
1.
de Almeida Engler J, De Vleesschauwer V, Burssens S, Celenza JL Jr, Inzé D, Van Montagu M, et al. Molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia. PLANT CELL. 1999;11(5):793–807.
MLA
de Almeida Engler, Janice, Vera De Vleesschauwer, Sylvia Burssens, et al. “Molecular Markers and Cell Cycle Inhibitors Show the Importance of Cell Cycle Progression in Nematode-induced Galls and Syncytia.” PLANT CELL 11.5 (1999): 793–807. Print.