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Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility

Marie Baucher UGent, Marie Andrée Bernard-Vailhé, Brigitte Chabbert, Jean-Michel Besle, Chris Opsomer, Marc Van Montagu UGent and Johan Botterman UGent (1999) PLANT MOLECULAR BIOLOGY. 39(3). p.437-447
abstract
To improve the digestibility of the forage crop alfalfa (Medicago sativa L.), cinnamyl alcohol dehydrogenase (CAD), which catalyses the last step in the biosynthesis of the lignin monomers, was down-regulated by using an antisense approach. A subset of six transgenic lines with reduced CAD activity and control lines were analysed when grown in the greenhouse and in the field. The down-regulation of the CAD enzyme was associated with a red coloration of the stem. The lignin quantity remained unchanged, but the lignin composition, as determined by thioacidolysis, was altered. The highest reduction of CAD activity was associated with a lower syringyl/guaicayl (S/G) ratio and a lower S+G yield, mainly because of a decreased amount of S units. An increase in in situ disappearance of dry matter and of cell wall residue was detected in one of the transgenic lines grown in the greenhouse, and for two of the lines grown in the field the rate of disappearance of dry matter slightly improved. Furthermore, these two lines had a higher solubility in alkali as shown by the lower yield of saponified residue. This study opens perspectives for improving forage crop digestibility by the modulation of enzymes involved in lignin biosynthesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SORGHUM, MAIZE, QUALITY, DIGESTION, antisense strategy, cinnamyl alcohol dehydrogenase, digestibility, lignin, Medicago sativa, ACID O-METHYLTRANSFERASE, BROWN-MIDRIB MUTANT, ANTISENSE GENE, PLANTS, POPLAR, EXPRESSION
journal title
PLANT MOLECULAR BIOLOGY
Plant Mol.Biol.
volume
39
issue
3
pages
437 - 447
Web of Science type
Article
Web of Science id
000079292100005
ISSN
0167-4412
DOI
10.1023/A:1006182925584
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
112463
handle
http://hdl.handle.net/1854/LU-112463
date created
2004-01-14 13:35:00
date last changed
2016-12-19 15:37:03
@article{112463,
  abstract     = {To improve the digestibility of the forage crop alfalfa (Medicago sativa L.), cinnamyl alcohol dehydrogenase (CAD), which catalyses the last step in the biosynthesis of the lignin monomers, was down-regulated by using an antisense approach. A subset of six transgenic lines with reduced CAD activity and control lines were analysed when grown in the greenhouse and in the field. The down-regulation of the CAD enzyme was associated with a red coloration of the stem. The lignin quantity remained unchanged, but the lignin composition, as determined by thioacidolysis, was altered. The highest reduction of CAD activity was associated with a lower syringyl/guaicayl (S/G) ratio and a lower S+G yield, mainly because of a decreased amount of S units. An increase in in situ disappearance of dry matter and of cell wall residue was detected in one of the transgenic lines grown in the greenhouse, and for two of the lines grown in the field the rate of disappearance of dry matter slightly improved. Furthermore, these two lines had a higher solubility in alkali as shown by the lower yield of saponified residue. This study opens perspectives for improving forage crop digestibility by the modulation of enzymes involved in lignin biosynthesis.},
  author       = {Baucher, Marie and Bernard-Vailh{\'e}, Marie Andr{\'e}e and Chabbert, Brigitte and Besle, Jean-Michel and Opsomer, Chris and Van Montagu, Marc and Botterman, Johan},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keyword      = {SORGHUM,MAIZE,QUALITY,DIGESTION,antisense strategy,cinnamyl alcohol dehydrogenase,digestibility,lignin,Medicago sativa,ACID O-METHYLTRANSFERASE,BROWN-MIDRIB MUTANT,ANTISENSE GENE,PLANTS,POPLAR,EXPRESSION},
  language     = {eng},
  number       = {3},
  pages        = {437--447},
  title        = {Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility},
  url          = {http://dx.doi.org/10.1023/A:1006182925584},
  volume       = {39},
  year         = {1999},
}

Chicago
Baucher, Marie, Marie Andrée Bernard-Vailhé, Brigitte Chabbert, Jean-Michel Besle, Chris Opsomer, Marc Van Montagu, and Johan Botterman. 1999. “Down-regulation of Cinnamyl Alcohol Dehydrogenase in Transgenic Alfalfa (Medicago Sativa L.) and the Effect on Lignin Composition and Digestibility.” Plant Molecular Biology 39 (3): 437–447.
APA
Baucher, M., Bernard-Vailhé, M. A., Chabbert, B., Besle, J.-M., Opsomer, C., Van Montagu, M., & Botterman, J. (1999). Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility. PLANT MOLECULAR BIOLOGY, 39(3), 437–447.
Vancouver
1.
Baucher M, Bernard-Vailhé MA, Chabbert B, Besle J-M, Opsomer C, Van Montagu M, et al. Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility. PLANT MOLECULAR BIOLOGY. 1999;39(3):437–47.
MLA
Baucher, Marie, Marie Andrée Bernard-Vailhé, Brigitte Chabbert, et al. “Down-regulation of Cinnamyl Alcohol Dehydrogenase in Transgenic Alfalfa (Medicago Sativa L.) and the Effect on Lignin Composition and Digestibility.” PLANT MOLECULAR BIOLOGY 39.3 (1999): 437–447. Print.