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74As-arsenate metabolism in Flemish Giant rabbits with renal insufficiency

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Abstract
The metabolic management of carrier-free As-74-arsenate (As(V)) by uremic rabbits of the strain Flemish Giant was studied. Renal insufficiency was induced by nephrectomy of respectively 1 kidney (3/6 nephrectomy) and 1 kidney + 2/3 remaining kidney (5/6 nephrectomy). Marginal renal insufficiency developed in the 3/6 nephrectomized group, while animals of the 5/6 group became severely uremic. Renal excretion of As-74 was reduced by 90% in 5/6 nephrectomized animals 4 h after intraperitoneal injection (i.p) of the animals. The associated uremic syndrome caused a strong decrease in methylation capacity of inorganic arsenic (As-i). The second methylation step from monomethylarsonic acid (MMA) to dimethylarsinic acid (DMA) was more strongly affected than the first one, from arsenite (As(III)) to MMA. The increased availability of As-i led to more extensive binding to insoluble tissue constituents after 5/6 nephrectomy. The decreased renal reduction of As(V) led to a decrease in As(III) and an increase of As(V) and the associated As(V)-transferrin binding in plasma. Uptake of As-74-transferrin by the bone marrow might contribute to uremic anemia.
Keywords
MECHANISM, CREATININE, ARSENATE, arsenate, ACCUMULATION, HYPOTHESIS, detoxification, uremic syndrome, METHYLATION, anemia, SERUM, HEMODIALYSIS, FAILURE, TOXICITY

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Citation

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Chicago
De Kimpe, Jurgen, Rita Cornelis, Louis Mees, Raymond Vanholder, and Geert Verhoeven. 1999. “74As-arsenate Metabolism in Flemish Giant Rabbits with Renal Insufficiency.” Journal of Trace Elements in Medicine and Biology 13 (1-2): 7–14.
APA
De Kimpe, Jurgen, Cornelis, R., Mees, L., Vanholder, R., & Verhoeven, G. (1999). 74As-arsenate metabolism in Flemish Giant rabbits with renal insufficiency. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, 13(1-2), 7–14.
Vancouver
1.
De Kimpe J, Cornelis R, Mees L, Vanholder R, Verhoeven G. 74As-arsenate metabolism in Flemish Giant rabbits with renal insufficiency. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY. 1999;13(1-2):7–14.
MLA
De Kimpe, Jurgen, Rita Cornelis, Louis Mees, et al. “74As-arsenate Metabolism in Flemish Giant Rabbits with Renal Insufficiency.” JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY 13.1-2 (1999): 7–14. Print.
@article{112255,
  abstract     = {The metabolic management of carrier-free As-74-arsenate (As(V)) by uremic rabbits of the strain Flemish Giant was studied. Renal insufficiency was induced by nephrectomy of respectively 1 kidney (3/6 nephrectomy) and 1 kidney + 2/3 remaining kidney (5/6 nephrectomy). Marginal renal insufficiency developed in the 3/6 nephrectomized group, while animals of the 5/6 group became severely uremic. Renal excretion of As-74 was reduced by 90\% in 5/6 nephrectomized animals 4 h after intraperitoneal injection (i.p) of the animals. The associated uremic syndrome caused a strong decrease in methylation capacity of inorganic arsenic (As-i). The second methylation step from monomethylarsonic acid (MMA) to dimethylarsinic acid (DMA) was more strongly affected than the first one, from arsenite (As(III)) to MMA. The increased availability of As-i led to more extensive binding to insoluble tissue constituents after 5/6 nephrectomy. The decreased renal reduction of As(V) led to a decrease in As(III) and an increase of As(V) and the associated As(V)-transferrin binding in plasma. Uptake of As-74-transferrin by the bone marrow might contribute to uremic anemia.},
  author       = {De Kimpe, Jurgen and Cornelis, Rita and Mees, Louis and Vanholder, Raymond and Verhoeven, Geert},
  issn         = {0946-672X},
  journal      = {JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY},
  keyword      = {MECHANISM,CREATININE,ARSENATE,arsenate,ACCUMULATION,HYPOTHESIS,detoxification,uremic syndrome,METHYLATION,anemia,SERUM,HEMODIALYSIS,FAILURE,TOXICITY},
  language     = {eng},
  number       = {1-2},
  pages        = {7--14},
  title        = {74As-arsenate metabolism in Flemish Giant rabbits with renal insufficiency},
  url          = {http://dx.doi.org/10.1016/S0946-672X(99)80017-0},
  volume       = {13},
  year         = {1999},
}

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