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Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans

Kristel Brys (UGent) , Natascha Castelein (UGent) , Filip Matthijssens (UGent) , Jacques Vanfleteren (UGent) and Bart Braeckman (UGent)
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Abstract
Background: The gene daf-2 encodes the single insulin/insulin growth factor-1-like receptor of Caenorhabditis elegans. The reduction-of-function allele e1370 induces several metabolic alterations and doubles lifespan. Results: We found that the e1370 mutation alters aerobic energy production substantially. In wild-type worms the abundance of key mitochondrial proteins declines with age, accompanied by a dramatic decrease in energy production, although the mitochondrial mass, inferred from the mitochondrial DNA copy number, remains unaltered. In contrast, the age-dependent decrease of both key mitochondrial proteins and bioenergetic competence is considerably attenuated in daf-2(e1370) adult animals. The increase in daf-2(e1370) mitochondrial competence is associated with a higher membrane potential and increased reactive oxygen species production, but with little damage to mitochondrial protein or DNA. Together these results point to a higher energetic efficiency of daf-2(e1370) animals. Conclusions: We conclude that low daf-2 function alters the overall rate of ageing by a yet unidentified mechanism with an indirect protective effect on mitochondrial function.
Keywords
LONG, OXIDATIVE STRESS, LONGEVITY, GENE-EXPRESSION, CLK MUTANTS, LIFE-SPAN DETERMINATION, DAMAGE THEORY, ELECTRON-TRANSPORT, LIVED DAF-2 MUTANTS, CYTOCHROME-C-OXIDASE

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Chicago
Brys, Kristel, Natascha Castelein, Filip Matthijssens, Jacques Vanfleteren, and Bart Braeckman. 2010. “Disruption of Insulin Signalling Preserves Bioenergetic Competence of Mitochondria in Ageing Caenorhabditis Elegans.” Bmc Biology 8.
APA
Brys, K., Castelein, N., Matthijssens, F., Vanfleteren, J., & Braeckman, B. (2010). Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans. BMC BIOLOGY, 8.
Vancouver
1.
Brys K, Castelein N, Matthijssens F, Vanfleteren J, Braeckman B. Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans. BMC BIOLOGY. 2010;8.
MLA
Brys, Kristel, Natascha Castelein, Filip Matthijssens, et al. “Disruption of Insulin Signalling Preserves Bioenergetic Competence of Mitochondria in Ageing Caenorhabditis Elegans.” BMC BIOLOGY 8 (2010): n. pag. Print.
@article{1114135,
  abstract     = {Background: The gene daf-2 encodes the single insulin/insulin growth factor-1-like receptor of Caenorhabditis elegans. The reduction-of-function allele e1370 induces several metabolic alterations and doubles lifespan.
Results: We found that the e1370 mutation alters aerobic energy production substantially. In wild-type worms the abundance of key mitochondrial proteins declines with age, accompanied by a dramatic decrease in energy production, although the mitochondrial mass, inferred from the mitochondrial DNA copy number, remains unaltered. In contrast, the age-dependent decrease of both key mitochondrial proteins and bioenergetic competence is considerably attenuated in daf-2(e1370) adult animals. The increase in daf-2(e1370) mitochondrial competence is associated with a higher membrane potential and increased reactive oxygen species production, but with little damage to mitochondrial protein or DNA. Together these results point to a higher energetic efficiency of daf-2(e1370) animals.
Conclusions: We conclude that low daf-2 function alters the overall rate of ageing by a yet unidentified mechanism with an indirect protective effect on mitochondrial function.},
  articleno    = {91},
  author       = {Brys, Kristel and Castelein, Natascha and Matthijssens, Filip and Vanfleteren, Jacques and Braeckman, Bart},
  issn         = {1741-7007},
  journal      = {BMC BIOLOGY},
  keyword      = {LONG,OXIDATIVE STRESS,LONGEVITY,GENE-EXPRESSION,CLK MUTANTS,LIFE-SPAN DETERMINATION,DAMAGE THEORY,ELECTRON-TRANSPORT,LIVED DAF-2 MUTANTS,CYTOCHROME-C-OXIDASE},
  language     = {eng},
  pages        = {15},
  title        = {Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans},
  url          = {http://dx.doi.org/10.1186/1741-7007-8-91},
  volume       = {8},
  year         = {2010},
}

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