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Lactoferrin inhibits E. coli O157: H7 growth and attachment to intestinal epithelial cells

Maryam Atef Yekta (UGent) , F Verdonck, Wim Van Den Broeck (UGent) , Bruno Goddeeris (UGent) , Eric Cox (UGent) and Daisy Vanrompay (UGent)
(2010) VETERINARNI MEDICINA. 55(8). p.359-368
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Abstract
Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. We studied the ability of bovine and human lactoferrin, two natural antimicrobial proteins present in milk, to inhibit E. coli O157:H7 growth and attachment to a human epithelial colorectal adenocarcinoma cell line (Caco-2). The direct antibacterial effect of bLF on E. coli O157:H7 was stronger than that of hLF. Nevertheless, both lactoferrins had bacteriostatic effects even at high concentrations (10 mg/ml), suggesting blocking of LF activity by a yet undefined bacterial defence mechanism. Additionally, both lactoferrins significantly inhibited E. coli O157:H7 attachment to Caco-2 cells. However, hLF was more effective than bLF, probably due to more efficient binding of bLF to intelectin present on human enterocytes leading to uptake and thus removal of bLF from the extracellular environment. Inhibition of bacterial attachment to Caco-2 cells was at least partly due to the catalytic effect of lactoferrins on the type III secreted proteins EspA and EspB
Keywords
HEMOLYTIC-UREMIC SYNDROME, GRAM-NEGATIVE BACTERIA, ENTEROHEMORRHAGIC ESCHERICHIA-COLI, TISSUE-CULTURE CELLS, BOVINE LACTOFERRIN, IN-VITRO, ANTIMICROBIAL PEPTIDES, BINDING-PROPERTIES, O157-H7 INFECTION, PROTEIN-A

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Citation

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Chicago
Atef Yekta, Maryam, F Verdonck, Wim Van Den Broeck, Bruno Goddeeris, Eric Cox, and Daisy Vanrompay. 2010. “Lactoferrin Inhibits E. Coli O157: H7 Growth and Attachment to Intestinal Epithelial Cells.” Veterinarni Medicina 55 (8): 359–368.
APA
Atef Yekta, M., Verdonck, F., Van Den Broeck, W., Goddeeris, B., Cox, E., & Vanrompay, D. (2010). Lactoferrin inhibits E. coli O157: H7 growth and attachment to intestinal epithelial cells. VETERINARNI MEDICINA, 55(8), 359–368.
Vancouver
1.
Atef Yekta M, Verdonck F, Van Den Broeck W, Goddeeris B, Cox E, Vanrompay D. Lactoferrin inhibits E. coli O157: H7 growth and attachment to intestinal epithelial cells. VETERINARNI MEDICINA. 2010;55(8):359–68.
MLA
Atef Yekta, Maryam, F Verdonck, Wim Van Den Broeck, et al. “Lactoferrin Inhibits E. Coli O157: H7 Growth and Attachment to Intestinal Epithelial Cells.” VETERINARNI MEDICINA 55.8 (2010): 359–368. Print.
@article{1109736,
  abstract     = {Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. We studied the ability of bovine and human lactoferrin, two natural antimicrobial proteins present in milk, to inhibit E. coli O157:H7 growth and attachment to a human epithelial colorectal adenocarcinoma cell line (Caco-2). The direct antibacterial effect of bLF on E. coli O157:H7 was stronger than that of hLF. Nevertheless, both lactoferrins had bacteriostatic effects even at high concentrations (10 mg/ml), suggesting blocking of LF activity by a yet undefined bacterial defence mechanism. Additionally, both lactoferrins significantly inhibited E. coli O157:H7 attachment to Caco-2 cells. However, hLF was more effective than bLF, probably due to more efficient binding of bLF to intelectin present on human enterocytes leading to uptake and thus removal of bLF from the extracellular environment. Inhibition of bacterial attachment to Caco-2 cells was at least partly due to the catalytic effect of lactoferrins on the type III secreted proteins EspA and EspB},
  author       = {Atef Yekta, Maryam and Verdonck, F and Van Den Broeck, Wim and Goddeeris, Bruno and Cox, Eric and Vanrompay, Daisy},
  issn         = {0375-8427},
  journal      = {VETERINARNI MEDICINA},
  keyword      = {HEMOLYTIC-UREMIC SYNDROME,GRAM-NEGATIVE BACTERIA,ENTEROHEMORRHAGIC ESCHERICHIA-COLI,TISSUE-CULTURE CELLS,BOVINE LACTOFERRIN,IN-VITRO,ANTIMICROBIAL PEPTIDES,BINDING-PROPERTIES,O157-H7 INFECTION,PROTEIN-A},
  language     = {eng},
  number       = {8},
  pages        = {359--368},
  title        = {Lactoferrin inhibits E. coli O157: H7 growth and attachment to intestinal epithelial cells},
  url          = {http://journals.uzpi.cz/web/\_generovani/vm\_2010.htm},
  volume       = {55},
  year         = {2010},
}

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