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The miR-17-92 MicroRNA Cluster regulates multiple components of the TGF-beta Pathway in neuroblastoma

(2010) MOLECULAR CELL. 40(5). p.762-773
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Abstract
The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a potent inhibitor of TGF-beta signaling. By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-beta signaling cascade as well as direct inhibition of TGF-beta-responsive genes.
Keywords
INDUCTION, NEURONAL DIFFERENTIATION, MALIGNANT PHENOTYPE, GENE TARGETS, EXPRESSION, INDUCTION, GROWTH, CANCER, PROLIFERATION, PROTEOMICS, BIOGENESIS

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MLA
Mestdagh, Pieter et al. “The miR-17-92 MicroRNA Cluster Regulates Multiple Components of the TGF-beta Pathway in Neuroblastoma.” MOLECULAR CELL 40.5 (2010): 762–773. Print.
APA
Mestdagh, P., Bostrom, A.-K., Impens, F., Fredlund, E., Van Peer, G., De Antonellis, P., von Stedingk, K., et al. (2010). The miR-17-92 MicroRNA Cluster regulates multiple components of the TGF-beta Pathway in neuroblastoma. MOLECULAR CELL, 40(5), 762–773.
Chicago author-date
Mestdagh, Pieter, Anna-Karin Bostrom, Francis Impens, Erik Fredlund, Gert Van Peer, Pasqualino De Antonellis, Kristoffer von Stedingk, et al. 2010. “The miR-17-92 MicroRNA Cluster Regulates Multiple Components of the TGF-beta Pathway in Neuroblastoma.” Molecular Cell 40 (5): 762–773.
Chicago author-date (all authors)
Mestdagh, Pieter, Anna-Karin Bostrom, Francis Impens, Erik Fredlund, Gert Van Peer, Pasqualino De Antonellis, Kristoffer von Stedingk, Bart Ghesquière, Stefanie Schulte, Michael Dews, Andrei Thomas-Tikhonenko, Johannes H Schulte, Massimo Zollo, Alexander Schramm, Kris Gevaert, Hakan Axelson, Franki Speleman, and Jo Vandesompele. 2010. “The miR-17-92 MicroRNA Cluster Regulates Multiple Components of the TGF-beta Pathway in Neuroblastoma.” Molecular Cell 40 (5): 762–773.
Vancouver
1.
Mestdagh P, Bostrom A-K, Impens F, Fredlund E, Van Peer G, De Antonellis P, et al. The miR-17-92 MicroRNA Cluster regulates multiple components of the TGF-beta Pathway in neuroblastoma. MOLECULAR CELL. 2010;40(5):762–73.
IEEE
[1]
P. Mestdagh et al., “The miR-17-92 MicroRNA Cluster regulates multiple components of the TGF-beta Pathway in neuroblastoma,” MOLECULAR CELL, vol. 40, no. 5, pp. 762–773, 2010.
@article{1109485,
  abstract     = {The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a potent inhibitor of TGF-beta signaling. By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-beta signaling cascade as well as direct inhibition of TGF-beta-responsive genes.},
  author       = {Mestdagh, Pieter and Bostrom, Anna-Karin and Impens, Francis and Fredlund, Erik and Van Peer, Gert and De Antonellis, Pasqualino and von Stedingk, Kristoffer and Ghesquière, Bart and Schulte, Stefanie and Dews, Michael and Thomas-Tikhonenko, Andrei and Schulte, Johannes H and Zollo, Massimo and Schramm, Alexander and Gevaert, Kris and Axelson, Hakan and Speleman, Franki and Vandesompele, Jo},
  issn         = {1097-2765},
  journal      = {MOLECULAR CELL},
  keywords     = {INDUCTION,NEURONAL DIFFERENTIATION,MALIGNANT PHENOTYPE,GENE TARGETS,EXPRESSION,INDUCTION,GROWTH,CANCER,PROLIFERATION,PROTEOMICS,BIOGENESIS},
  language     = {eng},
  number       = {5},
  pages        = {762--773},
  title        = {The miR-17-92 MicroRNA Cluster regulates multiple components of the TGF-beta Pathway in neuroblastoma},
  url          = {http://dx.doi.org/10.1016/j.molcel.2010.11.038},
  volume       = {40},
  year         = {2010},
}

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