Advanced search
1 file | 670.19 KB

Human faecal microbiota display variable patterns of glycerol metabolism

(2010) FEMS MICROBIOLOGY ECOLOGY. 74(3). p.601-611
Author
Organization
Abstract
Significant amounts of glycerol reach the colon microbiota daily through the diet and/or by in situ microbial production or release from desquamated epithelial cells. Some gut microorganisms may anaerobically reduce glycerol to 1,3-propanediol (1,3-PDO), with 3-hydroxypropanal as an intermediate. Accumulation of the latter intermediate may result in the formation of reuterin, which is known for its biological activity (e.g. antimicrobial properties). To date, glycerol metabolism in mixed cultures from the human colon has received little attention. Using in vitro batch incubations of faeces from 10 human individuals, we demonstrated that glycerol addition (140 mM) significantly affects the metabolism and composition of the microbial community. About a third of the samples exhibited rapid glycerol conversion, yielding proportionally higher levels of acetate and 1,3-PDO. In contrast, a slower glycerol metabolism resulted in higher levels of propionate. Furthermore, rapid glycerol metabolism correlated with significant shifts in the Lactobacillus-Enterococcus community, which were not observed in slower glycerol-metabolizing samples. As the conversion of glycerol to 1,3-PDO is a highly reducing process, we infer that the glycerol metabolism may act as an effective hydrogen sink. Given the importance of hydrogen-consuming processes in the gut, this work suggests that glycerol may have potential as a tool for modulating fermentation kinetics and profiles in the gastrointestinal tract.
Keywords
DIETARY FACTORS, PRODUCER PHENOTYPE, SMALL-INTESTINE, 3-HYDROXYPROPIONALDEHYDE, FERMENTATION, LACTOBACILLUS-REUTERI, CANCER CELL-LINE, RATIO MASS-SPECTROMETRY, LACTIC-ACID BACTERIA, CARRIER-MEDIATED TRANSPORT

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 670.19 KB

Citation

Please use this url to cite or link to this publication:

Chicago
De Weirdt, Rosemarie, Sam Possemiers, Griet Vermeulen, Tanja CW Moerdijk-Poortvliet, Henricus TS Boschker, Willy Verstraete, and Tom Van de Wiele. 2010. “Human Faecal Microbiota Display Variable Patterns of Glycerol Metabolism.” Fems Microbiology Ecology 74 (3): 601–611.
APA
De Weirdt, R., Possemiers, S., Vermeulen, G., Moerdijk-Poortvliet, T. C., Boschker, H. T., Verstraete, W., & Van de Wiele, T. (2010). Human faecal microbiota display variable patterns of glycerol metabolism. FEMS MICROBIOLOGY ECOLOGY, 74(3), 601–611.
Vancouver
1.
De Weirdt R, Possemiers S, Vermeulen G, Moerdijk-Poortvliet TC, Boschker HT, Verstraete W, et al. Human faecal microbiota display variable patterns of glycerol metabolism. FEMS MICROBIOLOGY ECOLOGY. 2010;74(3):601–11.
MLA
De Weirdt, Rosemarie, Sam Possemiers, Griet Vermeulen, et al. “Human Faecal Microbiota Display Variable Patterns of Glycerol Metabolism.” FEMS MICROBIOLOGY ECOLOGY 74.3 (2010): 601–611. Print.
@article{1108004,
  abstract     = {Significant amounts of glycerol reach the colon microbiota daily through the diet and/or by in situ microbial production or release from desquamated epithelial cells. Some gut microorganisms may anaerobically reduce glycerol to 1,3-propanediol (1,3-PDO), with 3-hydroxypropanal as an intermediate. Accumulation of the latter intermediate may result in the formation of reuterin, which is known for its biological activity (e.g. antimicrobial properties). To date, glycerol metabolism in mixed cultures from the human colon has received little attention. Using in vitro batch incubations of faeces from 10 human individuals, we demonstrated that glycerol addition (140 mM) significantly affects the metabolism and composition of the microbial community. About a third of the samples exhibited rapid glycerol conversion, yielding proportionally higher levels of acetate and 1,3-PDO. In contrast, a slower glycerol metabolism resulted in higher levels of propionate. Furthermore, rapid glycerol metabolism correlated with significant shifts in the Lactobacillus-Enterococcus community, which were not observed in slower glycerol-metabolizing samples. As the conversion of glycerol to 1,3-PDO is a highly reducing process, we infer that the glycerol metabolism may act as an effective hydrogen sink. Given the importance of hydrogen-consuming processes in the gut, this work suggests that glycerol may have potential as a tool for modulating fermentation kinetics and profiles in the gastrointestinal tract.},
  author       = {De Weirdt, Rosemarie and Possemiers, Sam and Vermeulen, Griet and Moerdijk-Poortvliet, Tanja CW and Boschker, Henricus TS and Verstraete, Willy and Van de Wiele, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {DIETARY FACTORS,PRODUCER PHENOTYPE,SMALL-INTESTINE,3-HYDROXYPROPIONALDEHYDE,FERMENTATION,LACTOBACILLUS-REUTERI,CANCER CELL-LINE,RATIO MASS-SPECTROMETRY,LACTIC-ACID BACTERIA,CARRIER-MEDIATED TRANSPORT},
  language     = {eng},
  number       = {3},
  pages        = {601--611},
  title        = {Human faecal microbiota display variable patterns of glycerol metabolism},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2010.00974.x},
  volume       = {74},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: