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Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Pieter Jacobs (UGent) , M Inan, Nele Festjens (UGent) , Jurgen Haustraete (UGent) , Annelies Van Hecke (UGent) , Roland Contreras (UGent) , MM Meagher and Nico Callewaert (UGent)
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Abstract
Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man(5)GlcNAc(2) N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.
Keywords
BIOLOGICAL-ACTIVITY, N-GLYCOSYLATION, OVINE INTERFERON-TAU, COLONY-STIMULATING FACTOR, ANIMAL-CELLS, YEAST, OPTIMIZATION, METHANOL, PROTEIN

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Chicago
Jacobs, Pieter, M Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, MM Meagher, and Nico Callewaert. 2010. “Fed-batch Fermentation of GM-CSF-producing Glycoengineered Pichia Pastoris Under Controlled Specific Growth Rate.” Microbial Cell Factories 9.
APA
Jacobs, Pieter, Inan, M., Festjens, N., Haustraete, J., Van Hecke, A., Contreras, R., Meagher, M., et al. (2010). Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. MICROBIAL CELL FACTORIES, 9.
Vancouver
1.
Jacobs P, Inan M, Festjens N, Haustraete J, Van Hecke A, Contreras R, et al. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. MICROBIAL CELL FACTORIES. 2010;9.
MLA
Jacobs, Pieter, M Inan, Nele Festjens, et al. “Fed-batch Fermentation of GM-CSF-producing Glycoengineered Pichia Pastoris Under Controlled Specific Growth Rate.” MICROBIAL CELL FACTORIES 9 (2010): n. pag. Print.
@article{1107777,
  abstract     = {Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce.
Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man(5)GlcNAc(2) N-glycosylation of the secreted proteins is achieved at all specific growth rates tested.
Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.},
  articleno    = {93},
  author       = {Jacobs, Pieter and Inan, M and Festjens, Nele and Haustraete, Jurgen and Van Hecke, Annelies and Contreras, Roland and Meagher, MM and Callewaert, Nico},
  issn         = {1475-2859},
  journal      = {MICROBIAL CELL FACTORIES},
  keyword      = {BIOLOGICAL-ACTIVITY,N-GLYCOSYLATION,OVINE INTERFERON-TAU,COLONY-STIMULATING FACTOR,ANIMAL-CELLS,YEAST,OPTIMIZATION,METHANOL,PROTEIN},
  language     = {eng},
  pages        = {9},
  title        = {Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate},
  url          = {http://dx.doi.org/10.1186/1475-2859-9-93},
  volume       = {9},
  year         = {2010},
}

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