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Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Pieter Jacobs UGent, M Inan, Nele Festjens UGent, Jurgen Haustraete UGent, Annelies Van Hecke UGent, Roland Contreras UGent, MM Meagher and Nico Callewaert UGent (2010) MICROBIAL CELL FACTORIES. 9.
abstract
Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man(5)GlcNAc(2) N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BIOLOGICAL-ACTIVITY, N-GLYCOSYLATION, OVINE INTERFERON-TAU, COLONY-STIMULATING FACTOR, ANIMAL-CELLS, YEAST, OPTIMIZATION, METHANOL, PROTEIN
journal title
MICROBIAL CELL FACTORIES
Microb. Cell. Fact.
volume
9
article_number
93
pages
9 pages
Web of Science type
Article
Web of Science id
000285468300001
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
4.544 (2010)
JCR rank
21/158 (2010)
JCR quartile
1 (2010)
ISSN
1475-2859
DOI
10.1186/1475-2859-9-93
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1107777
handle
http://hdl.handle.net/1854/LU-1107777
date created
2011-01-21 13:40:33
date last changed
2012-06-26 14:32:13
@article{1107777,
  abstract     = {Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce.
Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man(5)GlcNAc(2) N-glycosylation of the secreted proteins is achieved at all specific growth rates tested.
Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.},
  articleno    = {93},
  author       = {Jacobs, Pieter and Inan, M and Festjens, Nele and Haustraete, Jurgen and Van Hecke, Annelies and Contreras, Roland and Meagher, MM and Callewaert, Nico},
  issn         = {1475-2859},
  journal      = {MICROBIAL CELL FACTORIES},
  keyword      = {BIOLOGICAL-ACTIVITY,N-GLYCOSYLATION,OVINE INTERFERON-TAU,COLONY-STIMULATING FACTOR,ANIMAL-CELLS,YEAST,OPTIMIZATION,METHANOL,PROTEIN},
  language     = {eng},
  pages        = {9},
  title        = {Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate},
  url          = {http://dx.doi.org/10.1186/1475-2859-9-93},
  volume       = {9},
  year         = {2010},
}

Chicago
Jacobs, Pieter, M Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, MM Meagher, and Nico Callewaert. 2010. “Fed-batch Fermentation of GM-CSF-producing Glycoengineered Pichia Pastoris Under Controlled Specific Growth Rate.” Microbial Cell Factories 9.
APA
Jacobs, Pieter, Inan, M., Festjens, N., Haustraete, J., Van Hecke, A., Contreras, R., Meagher, M., et al. (2010). Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. MICROBIAL CELL FACTORIES, 9.
Vancouver
1.
Jacobs P, Inan M, Festjens N, Haustraete J, Van Hecke A, Contreras R, et al. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. MICROBIAL CELL FACTORIES. 2010;9.
MLA
Jacobs, Pieter, M Inan, Nele Festjens, et al. “Fed-batch Fermentation of GM-CSF-producing Glycoengineered Pichia Pastoris Under Controlled Specific Growth Rate.” MICROBIAL CELL FACTORIES 9 (2010): n. pag. Print.