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In vitro propagation of four saponin producing Maesa species

Ahmad Faizal UGent, Ellen Lambert UGent, Kenn Foubert, Sandra Apers and Danny Geelen UGent (2011) PLANT CELL TISSUE AND ORGAN CULTURE. 106(2). p.215-223
abstract
A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or alpha-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
flow cytometry, Maesa species, Adventitious shoot, saponin, conservation, ADVENTITIOUS SHOOT REGENERATION, LC-MS ANALYSIS, TISSUE-CULTURE, ANTILEISHMANIAL ACTIVITY, HIGH-FREQUENCY, 13, 28-EPOXY-OLEANANE SAPONINS, SOMACLONAL VARIATION, PLANT-REGENERATION, SPP. EXTRACTS, THIDIAZURON, axillary shoot
journal title
PLANT CELL TISSUE AND ORGAN CULTURE
Plant Cell Tissue Organ Cult.
volume
106
issue
2
pages
215 - 223
Web of Science type
Article
Web of Science id
000292989000004
JCR category
PLANT SCIENCES
JCR impact factor
3.09 (2011)
JCR rank
29/189 (2011)
JCR quartile
1 (2011)
ISSN
0167-6857
DOI
10.1007/s11240-010-9909-z
project
FWO-Flanders (project No. G.0014.08)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1106097
handle
http://hdl.handle.net/1854/LU-1106097
date created
2011-01-20 14:42:36
date last changed
2012-04-26 16:27:04
@article{1106097,
  abstract     = {A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or alpha-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100\% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins.},
  author       = {Faizal, Ahmad and Lambert, Ellen and Foubert, Kenn and Apers, Sandra and Geelen, Danny},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {flow cytometry,Maesa species,Adventitious shoot,saponin,conservation,ADVENTITIOUS SHOOT REGENERATION,LC-MS ANALYSIS,TISSUE-CULTURE,ANTILEISHMANIAL ACTIVITY,HIGH-FREQUENCY,13,28-EPOXY-OLEANANE SAPONINS,SOMACLONAL VARIATION,PLANT-REGENERATION,SPP. EXTRACTS,THIDIAZURON,axillary shoot},
  language     = {eng},
  number       = {2},
  pages        = {215--223},
  title        = {In vitro propagation of four saponin producing Maesa species},
  url          = {http://dx.doi.org/10.1007/s11240-010-9909-z},
  volume       = {106},
  year         = {2011},
}

Chicago
Faizal, Ahmad, Ellen Lambert, Kenn Foubert, Sandra Apers, and Danny Geelen. 2011. “In Vitro Propagation of Four Saponin Producing Maesa Species.” Plant Cell Tissue and Organ Culture 106 (2): 215–223.
APA
Faizal, A., Lambert, E., Foubert, K., Apers, S., & Geelen, D. (2011). In vitro propagation of four saponin producing Maesa species. PLANT CELL TISSUE AND ORGAN CULTURE, 106(2), 215–223.
Vancouver
1.
Faizal A, Lambert E, Foubert K, Apers S, Geelen D. In vitro propagation of four saponin producing Maesa species. PLANT CELL TISSUE AND ORGAN CULTURE. 2011;106(2):215–23.
MLA
Faizal, Ahmad, Ellen Lambert, Kenn Foubert, et al. “In Vitro Propagation of Four Saponin Producing Maesa Species.” PLANT CELL TISSUE AND ORGAN CULTURE 106.2 (2011): 215–223. Print.