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In vitro propagation of four saponin producing Maesa species

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Organization
Project
FWO-Flanders (project No. G.0014.08)
Abstract
A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or alpha-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins.
Keywords
flow cytometry, Maesa species, Adventitious shoot, saponin, conservation, ADVENTITIOUS SHOOT REGENERATION, LC-MS ANALYSIS, TISSUE-CULTURE, ANTILEISHMANIAL ACTIVITY, HIGH-FREQUENCY, 13, 28-EPOXY-OLEANANE SAPONINS, SOMACLONAL VARIATION, PLANT-REGENERATION, SPP. EXTRACTS, THIDIAZURON, axillary shoot

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Citation

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Chicago
Faizal, Ahmad, Ellen Lambert, Kenn Foubert, Sandra Apers, and Danny Geelen. 2011. “In Vitro Propagation of Four Saponin Producing Maesa Species.” Plant Cell Tissue and Organ Culture 106 (2): 215–223.
APA
Faizal, A., Lambert, E., Foubert, K., Apers, S., & Geelen, D. (2011). In vitro propagation of four saponin producing Maesa species. PLANT CELL TISSUE AND ORGAN CULTURE, 106(2), 215–223.
Vancouver
1.
Faizal A, Lambert E, Foubert K, Apers S, Geelen D. In vitro propagation of four saponin producing Maesa species. PLANT CELL TISSUE AND ORGAN CULTURE. 2011;106(2):215–23.
MLA
Faizal, Ahmad, Ellen Lambert, Kenn Foubert, et al. “In Vitro Propagation of Four Saponin Producing Maesa Species.” PLANT CELL TISSUE AND ORGAN CULTURE 106.2 (2011): 215–223. Print.
@article{1106097,
  abstract     = {A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or alpha-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100\% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins.},
  author       = {Faizal, Ahmad and Lambert, Ellen and Foubert, Kenn and Apers, Sandra and Geelen, Danny},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {flow cytometry,Maesa species,Adventitious shoot,saponin,conservation,ADVENTITIOUS SHOOT REGENERATION,LC-MS ANALYSIS,TISSUE-CULTURE,ANTILEISHMANIAL ACTIVITY,HIGH-FREQUENCY,13,28-EPOXY-OLEANANE SAPONINS,SOMACLONAL VARIATION,PLANT-REGENERATION,SPP. EXTRACTS,THIDIAZURON,axillary shoot},
  language     = {eng},
  number       = {2},
  pages        = {215--223},
  title        = {In vitro propagation of four saponin producing Maesa species},
  url          = {http://dx.doi.org/10.1007/s11240-010-9909-z},
  volume       = {106},
  year         = {2011},
}

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