Advanced search
1 file | 1.81 MB

Enhancing the microbial conversion of glycerol to 1,3-propanediol using metabolic engineering

Veerle Maervoet (UGent) , Marjan De Mey (UGent) , Joeri Beauprez (UGent) , Sofie De Maeseneire (UGent) and Wim Soetaert (UGent)
Author
Organization
Project
Biotechnology for a sustainable economy (Bio-Economy)
Abstract
1,3-propanediol (PDO) is the starting point of a new generation polymer with superior properties which is used in the textile and carpet industry. This product is mainly produced chemically, but high pressure, high temperature and expensive catalysts are required and toxic intermediates are released. Therefore, the biological production route has been studied intensively. DuPont and Genencor International, Inc. have modified E. coli genetically so that this organism could produce PDO from glucose. However, due to the tremendous growth of the biofuel industry, a glycerol surplus has been created, so more and more researchers started to investigate the natural producing strains for the production of PDO from glycerol. Several metabolic engineering techniques have been used to enhance the production of PDO. This review gives an overview of the different strategies to increase the final titer, yield and productivity of 1,3-propanediol in non-natural and natural producing strains.
Keywords
metabolic engineering, glycerol, 3-propanediol, 1, Clostridium sp., Klebsiella sp., TRANSPORTER CLASSIFICATION DATABASE, COENZYME B-12-DEPENDENT GLYCEROL, SUCCINIC ACID PRODUCTION, BY-PRODUCT FORMATION, KLEBSIELLA-PNEUMONIAE, ESCHERICHIA-COLI, CLOSTRIDIUM-ACETOBUTYLICUM, 3-HYDROXYPROPIONALDEHYDE ACCUMULATION, ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS, LACTOBACILLUS-PLANTARUM

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.81 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Maervoet, Veerle, Marjan De Mey, Joeri Beauprez, Sofie De Maeseneire, and Wim Soetaert. 2011. “Enhancing the Microbial Conversion of Glycerol to 1,3-propanediol Using Metabolic Engineering.” Organic Process Research & Development 15 (1): 189–202.
APA
Maervoet, V., De Mey, M., Beauprez, J., De Maeseneire, S., & Soetaert, W. (2011). Enhancing the microbial conversion of glycerol to 1,3-propanediol using metabolic engineering. ORGANIC PROCESS RESEARCH & DEVELOPMENT, 15(1), 189–202.
Vancouver
1.
Maervoet V, De Mey M, Beauprez J, De Maeseneire S, Soetaert W. Enhancing the microbial conversion of glycerol to 1,3-propanediol using metabolic engineering. ORGANIC PROCESS RESEARCH & DEVELOPMENT. 2011;15(1):189–202.
MLA
Maervoet, Veerle, Marjan De Mey, Joeri Beauprez, et al. “Enhancing the Microbial Conversion of Glycerol to 1,3-propanediol Using Metabolic Engineering.” ORGANIC PROCESS RESEARCH & DEVELOPMENT 15.1 (2011): 189–202. Print.
@article{1101830,
  abstract     = {1,3-propanediol (PDO) is the starting point of a new generation polymer with superior properties which is used in the textile and carpet industry. This product is mainly produced chemically, but high pressure, high temperature and expensive catalysts are required and toxic intermediates are released. Therefore, the biological production route has been studied intensively. DuPont and Genencor International, Inc. have modified E. coli genetically so that this organism could produce PDO from glucose. However, due to the tremendous growth of the biofuel industry, a glycerol surplus has been created, so more and more researchers started to investigate the natural producing strains for the production of PDO from glycerol. Several metabolic engineering techniques have been used to enhance the production of PDO. This review gives an overview of the different strategies to increase the final titer, yield and productivity of 1,3-propanediol in non-natural and natural producing strains.},
  author       = {Maervoet, Veerle and De Mey, Marjan and Beauprez, Joeri and De Maeseneire, Sofie and Soetaert, Wim},
  issn         = {1083-6160},
  journal      = {ORGANIC PROCESS RESEARCH & DEVELOPMENT},
  keywords     = {metabolic engineering,glycerol,3-propanediol,1,Clostridium sp.,Klebsiella sp.,TRANSPORTER CLASSIFICATION DATABASE,COENZYME B-12-DEPENDENT GLYCEROL,SUCCINIC ACID PRODUCTION,BY-PRODUCT FORMATION,KLEBSIELLA-PNEUMONIAE,ESCHERICHIA-COLI,CLOSTRIDIUM-ACETOBUTYLICUM,3-HYDROXYPROPIONALDEHYDE ACCUMULATION,ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS,LACTOBACILLUS-PLANTARUM},
  language     = {eng},
  number       = {1},
  pages        = {189--202},
  title        = {Enhancing the microbial conversion of glycerol to 1,3-propanediol using metabolic engineering},
  url          = {http://dx.doi.org/10.1021/op1001929},
  volume       = {15},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: