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Multiplexed profiling of secreted proteins for the detection of potential space biomarkers

(2011) MOLECULAR MEDICINE REPORTS. 4(1). p.17-23
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PRODEX/ESA/BELSPO (C90-298, C90-330, C90-303, C90-380 and C90-391)
Abstract
Space travel exposes astronauts to a plethora of potentially detrimental conditions, such as cosmic radiation and microgravity. As both factors are hard to simulate on Earth, present knowledge remains limited. However, this knowledge is of vital importance, making space flight experiments a necessity for determining the biological effects and the underlying biochemical processes, especially when keeping future long-term interplanetary missions in mind. Instead of estimating the long-term effects, which usually implicate severe endpoints (e.g., cancer) and which are often difficult to attribute, research has shifted to finding representative biomarkers for rapid and sensitive detection of individual radiosensitivity. In this context, an appealing set of candidate markers is the group of secreted proteins, as they exert an intercellular signaling function and are easy to assess. We screened a subset of secreted proteins in cells exposed to space travel by means of multiplex bead array analysis. To determine the cell-specific signatures of the secreted molecules, we compared the conditioned medium of normal fibroblast cells to fibroblasts isolated from a patient with Hutchinson-Gilford Progeria syndrome, which are known to have a perturbed nuclear architecture and DNA damage response. Out of the 88 molecules screened, 20 showed a significant level increase or decrease, with a differential response to space conditions between the two cell types. Among the molecules that were retained, which may prove to be valuable biomarkers, are apolipoprotein C-III, plasminogen activator inhibitor type 1, beta-2-microglobulin, ferritin, MMP-3, TIMP-1 and VEGF.
Keywords
cytokines, biomarker, space, microgravity, radiation, PRELAMIN-A, RADIATION, INFLAMMATION, ASTRONAUTS, DEFICIENCY, CYTOKINES, EXPOSURE, STATION, INJURY, SERUM

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Citation

Please use this url to cite or link to this publication:

Chicago
Dieriks, Birger, Winnok De Vos, Marjan Moreels, Myriam Ghardi, Raoul Hennekam, Jos LV Broers, Sarah Baatout, and Patric Van Oostveldt. 2011. “Multiplexed Profiling of Secreted Proteins for the Detection of Potential Space Biomarkers.” Molecular Medicine Reports 4 (1): 17–23.
APA
Dieriks, Birger, De Vos, W., Moreels, M., Ghardi, M., Hennekam, R., Broers, J. L., Baatout, S., et al. (2011). Multiplexed profiling of secreted proteins for the detection of potential space biomarkers. MOLECULAR MEDICINE REPORTS, 4(1), 17–23.
Vancouver
1.
Dieriks B, De Vos W, Moreels M, Ghardi M, Hennekam R, Broers JL, et al. Multiplexed profiling of secreted proteins for the detection of potential space biomarkers. MOLECULAR MEDICINE REPORTS. 2011;4(1):17–23.
MLA
Dieriks, Birger, Winnok De Vos, Marjan Moreels, et al. “Multiplexed Profiling of Secreted Proteins for the Detection of Potential Space Biomarkers.” MOLECULAR MEDICINE REPORTS 4.1 (2011): 17–23. Print.
@article{1100998,
  abstract     = {Space travel exposes astronauts to a plethora of potentially detrimental conditions, such as cosmic radiation and microgravity. As both factors are hard to simulate on Earth, present knowledge remains limited. However, this knowledge is of vital importance, making space flight experiments a necessity for determining the biological effects and the underlying biochemical processes, especially when keeping future long-term interplanetary missions in mind. Instead of estimating the long-term effects, which usually implicate severe endpoints (e.g., cancer) and which are often difficult to attribute, research has shifted to finding representative biomarkers for rapid and sensitive detection of individual radiosensitivity. In this context, an appealing set of candidate markers is the group of secreted proteins, as they exert an intercellular signaling function and are easy to assess. We screened a subset of secreted proteins in cells exposed to space travel by means of multiplex bead array analysis. To determine the cell-specific signatures of the secreted molecules, we compared the conditioned medium of normal fibroblast cells to fibroblasts isolated from a patient with Hutchinson-Gilford Progeria syndrome, which are known to have a perturbed nuclear architecture and DNA damage response. Out of the 88 molecules screened, 20 showed a significant level increase or decrease, with a differential response to space conditions between the two cell types. Among the molecules that were retained, which may prove to be valuable biomarkers, are apolipoprotein C-III, plasminogen activator inhibitor type 1, beta-2-microglobulin, ferritin, MMP-3, TIMP-1 and VEGF.},
  author       = {Dieriks, Birger and De Vos, Winnok and Moreels, Marjan  and Ghardi, Myriam and Hennekam, Raoul and Broers, Jos LV and Baatout, Sarah and Van Oostveldt, Patric},
  issn         = {1791-2997},
  journal      = {MOLECULAR MEDICINE REPORTS},
  keyword      = {cytokines,biomarker,space,microgravity,radiation,PRELAMIN-A,RADIATION,INFLAMMATION,ASTRONAUTS,DEFICIENCY,CYTOKINES,EXPOSURE,STATION,INJURY,SERUM},
  language     = {eng},
  number       = {1},
  pages        = {17--23},
  title        = {Multiplexed profiling of secreted proteins for the detection of potential space biomarkers},
  url          = {http://dx.doi.org/10.3892/mmr.2010.405},
  volume       = {4},
  year         = {2011},
}

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