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Multiplexed profiling of secreted proteins for the detection of potential space biomarkers

Birger Dieriks UGent, Winnok De Vos UGent, Marjan Moreels, Myriam Ghardi, Raoul Hennekam, Jos LV Broers, Sarah Baatout UGent and Patric Van Oostveldt UGent (2011) MOLECULAR MEDICINE REPORTS. 4(1). p.17-23
abstract
Space travel exposes astronauts to a plethora of potentially detrimental conditions, such as cosmic radiation and microgravity. As both factors are hard to simulate on Earth, present knowledge remains limited. However, this knowledge is of vital importance, making space flight experiments a necessity for determining the biological effects and the underlying biochemical processes, especially when keeping future long-term interplanetary missions in mind. Instead of estimating the long-term effects, which usually implicate severe endpoints (e.g., cancer) and which are often difficult to attribute, research has shifted to finding representative biomarkers for rapid and sensitive detection of individual radiosensitivity. In this context, an appealing set of candidate markers is the group of secreted proteins, as they exert an intercellular signaling function and are easy to assess. We screened a subset of secreted proteins in cells exposed to space travel by means of multiplex bead array analysis. To determine the cell-specific signatures of the secreted molecules, we compared the conditioned medium of normal fibroblast cells to fibroblasts isolated from a patient with Hutchinson-Gilford Progeria syndrome, which are known to have a perturbed nuclear architecture and DNA damage response. Out of the 88 molecules screened, 20 showed a significant level increase or decrease, with a differential response to space conditions between the two cell types. Among the molecules that were retained, which may prove to be valuable biomarkers, are apolipoprotein C-III, plasminogen activator inhibitor type 1, beta-2-microglobulin, ferritin, MMP-3, TIMP-1 and VEGF.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cytokines, biomarker, space, microgravity, radiation, PRELAMIN-A, RADIATION, INFLAMMATION, ASTRONAUTS, DEFICIENCY, CYTOKINES, EXPOSURE, STATION, INJURY, SERUM
journal title
MOLECULAR MEDICINE REPORTS
Mol. Med. Rep.
volume
4
issue
1
pages
17 - 23
Web of Science type
Article
Web of Science id
000286301100003
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
0.418 (2011)
JCR rank
98/109 (2011)
JCR quartile
4 (2011)
ISSN
1791-2997
DOI
10.3892/mmr.2010.405
project
PRODEX/ESA/BELSPO (C90-298, C90-330, C90-303, C90-380 and C90-391)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1100998
handle
http://hdl.handle.net/1854/LU-1100998
date created
2011-01-17 11:19:14
date last changed
2012-05-02 14:32:15
@article{1100998,
  abstract     = {Space travel exposes astronauts to a plethora of potentially detrimental conditions, such as cosmic radiation and microgravity. As both factors are hard to simulate on Earth, present knowledge remains limited. However, this knowledge is of vital importance, making space flight experiments a necessity for determining the biological effects and the underlying biochemical processes, especially when keeping future long-term interplanetary missions in mind. Instead of estimating the long-term effects, which usually implicate severe endpoints (e.g., cancer) and which are often difficult to attribute, research has shifted to finding representative biomarkers for rapid and sensitive detection of individual radiosensitivity. In this context, an appealing set of candidate markers is the group of secreted proteins, as they exert an intercellular signaling function and are easy to assess. We screened a subset of secreted proteins in cells exposed to space travel by means of multiplex bead array analysis. To determine the cell-specific signatures of the secreted molecules, we compared the conditioned medium of normal fibroblast cells to fibroblasts isolated from a patient with Hutchinson-Gilford Progeria syndrome, which are known to have a perturbed nuclear architecture and DNA damage response. Out of the 88 molecules screened, 20 showed a significant level increase or decrease, with a differential response to space conditions between the two cell types. Among the molecules that were retained, which may prove to be valuable biomarkers, are apolipoprotein C-III, plasminogen activator inhibitor type 1, beta-2-microglobulin, ferritin, MMP-3, TIMP-1 and VEGF.},
  author       = {Dieriks, Birger and De Vos, Winnok and Moreels, Marjan  and Ghardi, Myriam and Hennekam, Raoul and Broers, Jos LV and Baatout, Sarah and Van Oostveldt, Patric},
  issn         = {1791-2997},
  journal      = {MOLECULAR MEDICINE REPORTS},
  keyword      = {cytokines,biomarker,space,microgravity,radiation,PRELAMIN-A,RADIATION,INFLAMMATION,ASTRONAUTS,DEFICIENCY,CYTOKINES,EXPOSURE,STATION,INJURY,SERUM},
  language     = {eng},
  number       = {1},
  pages        = {17--23},
  title        = {Multiplexed profiling of secreted proteins for the detection of potential space biomarkers},
  url          = {http://dx.doi.org/10.3892/mmr.2010.405},
  volume       = {4},
  year         = {2011},
}

Chicago
Dieriks, Birger, Winnok De Vos, Marjan Moreels, Myriam Ghardi, Raoul Hennekam, Jos LV Broers, Sarah Baatout, and Patric Van Oostveldt. 2011. “Multiplexed Profiling of Secreted Proteins for the Detection of Potential Space Biomarkers.” Molecular Medicine Reports 4 (1): 17–23.
APA
Dieriks, B., De Vos, W., Moreels, M., Ghardi, M., Hennekam, R., Broers, J. L., Baatout, S., et al. (2011). Multiplexed profiling of secreted proteins for the detection of potential space biomarkers. MOLECULAR MEDICINE REPORTS, 4(1), 17–23.
Vancouver
1.
Dieriks B, De Vos W, Moreels M, Ghardi M, Hennekam R, Broers JL, et al. Multiplexed profiling of secreted proteins for the detection of potential space biomarkers. MOLECULAR MEDICINE REPORTS. 2011;4(1):17–23.
MLA
Dieriks, Birger, Winnok De Vos, Marjan Moreels, et al. “Multiplexed Profiling of Secreted Proteins for the Detection of Potential Space Biomarkers.” MOLECULAR MEDICINE REPORTS 4.1 (2011): 17–23. Print.