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Introns reduce transitivity proportionally to their length, suggesting that silencing spreads along the pre-mRNA

Leen Vermeersch (UGent), Nancy De Winne (UGent) and Anna Depicker (UGent)
(2010) PLANT JOURNAL. 64(3). p.392-401
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Abstract
Endogenes rarely support transitive silencing, whereas most transgenes generally allow the spread of silencing to occur along the primary target. To determine whether the presence of introns might explain the difference, we investigated the influence of introns in the primary target on 3'-5' silencing transitivity. When present in a transgene, an intron-containing endogene fragment does not prohibit the spread of silencing across this fragment, indicating that introns do not preclude silencing transitivity along endogenes. Also, a multiple intron-containing genomic gene fragment that had previously been shown not to support transitivity in an endogenous context could support transitivity when present in a transgene. Nevertheless, genomic intron-containing fragments delayed the onset and diminished the efficiency of transitive silencing of a secondary target compared with the corresponding cDNA fragments. Remarkably, transitivity was impaired proportionally with the length of the pre-mRNA, and not of the mRNA. The latter result suggests that the RNA-dependent RNA polymerase-based spreading of silencing progresses along the non-spliced rather than the fully processed mature mRNA.
Keywords
intron, transitivity, RNA interference, TOBACCO, METHYLATION, ACCUMULATION, SMALL ANTISENSE RNAS, BETA-1, TRANS-ACTING SIRNAS, TRANSFORMATION, AMPLIFICATION, 3-GLUCANASE GENES, ARABIDOPSIS-THALIANA, SUPPRESSION, chimeric primary target, Arabidopsis thaliana, inverted repeat locus

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Chicago
Vermeersch, Leen, Nancy De Winne, and Anna Depicker. 2010. “Introns Reduce Transitivity Proportionally to Their Length, Suggesting That Silencing Spreads Along the pre-mRNA.” Plant Journal 64 (3): 392–401.
APA
Vermeersch, Leen, De Winne, N., & Depicker, A. (2010). Introns reduce transitivity proportionally to their length, suggesting that silencing spreads along the pre-mRNA. PLANT JOURNAL, 64(3), 392–401.
Vancouver
1.
Vermeersch L, De Winne N, Depicker A. Introns reduce transitivity proportionally to their length, suggesting that silencing spreads along the pre-mRNA. PLANT JOURNAL. 2010;64(3):392–401.
MLA
Vermeersch, Leen, Nancy De Winne, and Anna Depicker. “Introns Reduce Transitivity Proportionally to Their Length, Suggesting That Silencing Spreads Along the pre-mRNA.” PLANT JOURNAL 64.3 (2010): 392–401. Print.
@article{1093035,
  abstract     = {Endogenes rarely support transitive silencing, whereas most transgenes generally allow the spread of silencing to occur along the primary target. To determine whether the presence of introns might explain the difference, we investigated the influence of introns in the primary target on 3'-5' silencing transitivity. When present in a transgene, an intron-containing endogene fragment does not prohibit the spread of silencing across this fragment, indicating that introns do not preclude silencing transitivity along endogenes. Also, a multiple intron-containing genomic gene fragment that had previously been shown not to support transitivity in an endogenous context could support transitivity when present in a transgene. Nevertheless, genomic intron-containing fragments delayed the onset and diminished the efficiency of transitive silencing of a secondary target compared with the corresponding cDNA fragments. Remarkably, transitivity was impaired proportionally with the length of the pre-mRNA, and not of the mRNA. The latter result suggests that the RNA-dependent RNA polymerase-based spreading of silencing progresses along the non-spliced rather than the fully processed mature mRNA.},
  author       = {Vermeersch, Leen and De Winne, Nancy and Depicker, Anna},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {intron,transitivity,RNA interference,TOBACCO,METHYLATION,ACCUMULATION,SMALL ANTISENSE RNAS,BETA-1,TRANS-ACTING SIRNAS,TRANSFORMATION,AMPLIFICATION,3-GLUCANASE GENES,ARABIDOPSIS-THALIANA,SUPPRESSION,chimeric primary target,Arabidopsis thaliana,inverted repeat locus},
  language     = {eng},
  number       = {3},
  pages        = {392--401},
  title        = {Introns reduce transitivity proportionally to their length, suggesting that silencing spreads along the pre-mRNA},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2010.04335.x},
  volume       = {64},
  year         = {2010},
}

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