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SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle

Stijn Dhondt UGent, Frederik Coppens UGent, Freya De Winter UGent, Kamal Swarup, Roeland Merks UGent, Dirk Inzé UGent, Malcolm J Bennett and Gerrit Beemster UGent (2010) PLANT PHYSIOLOGY. 154(3). p.1183-1195
abstract
SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root growth. This paper reports on the role of SHR and SCR in the development of leaves, which, in contrast to the root, have a determinate growth pattern and lack a persistent stem cell niche. Our results demonstrate that inhibition of leaf growth in shr and scr mutants is not a secondary effect of the compromised root development but is caused by an effect on cell division in the leaves: a reduced cell division rate and early exit of the proliferation phase. Consistent with the observed cell division phenotype, the expression of SHR and SCR genes in leaves is closely associated with cell division activity in most cell types. The increased cell cycle duration is due to a prolonged S-phase duration, which is mediated by up-regulation of cell cycle inhibitors known to restrain the activity of the transcription factor, E2Fa. Therefore, we conclude that, in contrast to their specific roles in cortex/endodermis differentiation and stem cell maintenance in the root, SHR and SCR primarily function as general regulators of cell proliferation in leaves.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SHOOT GRAVITROPISM, ORGAN SIZE, NF-YA, EXPRESSION, THALIANA, TRANSCRIPTION FACTORS, E2F TARGET GENES, GENOME-WIDE IDENTIFICATION, RETINOBLASTOMA/E2F PATHWAY, RADIAL ORGANIZATION
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
154
issue
3
pages
1183 - 1195
Web of Science type
Article
Web of Science id
000283710300015
JCR category
PLANT SCIENCES
JCR impact factor
6.451 (2010)
JCR rank
8/185 (2010)
JCR quartile
1 (2010)
ISSN
0032-0889
DOI
10.1104/pp.110.158857
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1092968
handle
http://hdl.handle.net/1854/LU-1092968
date created
2010-12-28 18:42:59
date last changed
2012-09-19 14:03:45
@article{1092968,
  abstract     = {SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root growth. This paper reports on the role of SHR and SCR in the development of leaves, which, in contrast to the root, have a determinate growth pattern and lack a persistent stem cell niche. Our results demonstrate that inhibition of leaf growth in shr and scr mutants is not a secondary effect of the compromised root development but is caused by an effect on cell division in the leaves: a reduced cell division rate and early exit of the proliferation phase. Consistent with the observed cell division phenotype, the expression of SHR and SCR genes in leaves is closely associated with cell division activity in most cell types. The increased cell cycle duration is due to a prolonged S-phase duration, which is mediated by up-regulation of cell cycle inhibitors known to restrain the activity of the transcription factor, E2Fa. Therefore, we conclude that, in contrast to their specific roles in cortex/endodermis differentiation and stem cell maintenance in the root, SHR and SCR primarily function as general regulators of cell proliferation in leaves.},
  author       = {Dhondt, Stijn and Coppens, Frederik and De Winter, Freya and Swarup, Kamal and Merks, Roeland and Inz{\'e}, Dirk and Bennett, Malcolm J and Beemster, Gerrit},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {SHOOT GRAVITROPISM,ORGAN SIZE,NF-YA,EXPRESSION,THALIANA,TRANSCRIPTION FACTORS,E2F TARGET GENES,GENOME-WIDE IDENTIFICATION,RETINOBLASTOMA/E2F PATHWAY,RADIAL ORGANIZATION},
  language     = {eng},
  number       = {3},
  pages        = {1183--1195},
  title        = {SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle},
  url          = {http://dx.doi.org/10.1104/pp.110.158857},
  volume       = {154},
  year         = {2010},
}

Chicago
Dhondt, Stijn, Frederik Coppens, Freya De Winter, Kamal Swarup, Roeland Merks, Dirk Inzé, Malcolm J Bennett, and Gerrit Beemster. 2010. “SHORT-ROOT and SCARECROW Regulate Leaf Growth in Arabidopsis by Stimulating S-phase Progression of the Cell Cycle.” Plant Physiology 154 (3): 1183–1195.
APA
Dhondt, S., Coppens, F., De Winter, F., Swarup, K., Merks, R., Inzé, D., Bennett, M. J., et al. (2010). SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle. PLANT PHYSIOLOGY, 154(3), 1183–1195.
Vancouver
1.
Dhondt S, Coppens F, De Winter F, Swarup K, Merks R, Inzé D, et al. SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle. PLANT PHYSIOLOGY. 2010;154(3):1183–95.
MLA
Dhondt, Stijn, Frederik Coppens, Freya De Winter, et al. “SHORT-ROOT and SCARECROW Regulate Leaf Growth in Arabidopsis by Stimulating S-phase Progression of the Cell Cycle.” PLANT PHYSIOLOGY 154.3 (2010): 1183–1195. Print.