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A replication stress-induced synchronization method for Arabidopsis thaliana root meristems

Toon Cools, Anelia Iantcheva, Sara Maes UGent, Hilde Van Den Daele UGent and Lieven De Veylder UGent (2010) PLANT JOURNAL. 64(4). p.705-714
abstract
Synchronized cell cultures are an indispensable tool for the identification and understanding of key regulators of the cell cycle. Nevertheless, the use of cell cultures has its disadvantages, because it represents an artificial system that does not completely mimic the endogenous conditions that occur in organized meristems. Here, we present a new and easy method for Arabidopsis thaliana root tip synchronization by hydroxyurea treatment. A major advantage of the method is the possibility of investigating available Arabidopsis cell-cycle mutants without the need to generate cell cultures. As a proof of concept, the effects of over-expression of a dominant negative allele of the B-type cyclin-dependent kinase CDKB1;1 gene on cell-cycle progression were tested. The previously observed prolonged G(2) phase was confirmed, but was found to be compensated for by a reduced G(1) phase. Furthermore, altered S-phase kinetics indicated a functional role for CDKB1;1 during the replication process.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cell cycle, Arabidopsis thaliana, CDKB1, 1, hydroxyurea, root meristem, synchronization, CYCLIN-DEPENDENT KINASES, GENE-EXPRESSION, CELL-DIVISION, TRANSCRIPTION FACTOR, PLANT DEVELOPMENT, GENOTOXIC STRESS, DNA-DAMAGE, S-PERIOD, PROGRESSION, COMPLEXES
journal title
PLANT JOURNAL
Plant J.
volume
64
issue
4
pages
705 - 714
Web of Science type
Article
Web of Science id
000284122400014
JCR category
PLANT SCIENCES
JCR impact factor
6.948 (2010)
JCR rank
6/185 (2010)
JCR quartile
1 (2010)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2010.04361.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1092961
handle
http://hdl.handle.net/1854/LU-1092961
date created
2010-12-28 18:42:37
date last changed
2016-12-19 15:46:17
@article{1092961,
  abstract     = {Synchronized cell cultures are an indispensable tool for the identification and understanding of key regulators of the cell cycle. Nevertheless, the use of cell cultures has its disadvantages, because it represents an artificial system that does not completely mimic the endogenous conditions that occur in organized meristems. Here, we present a new and easy method for Arabidopsis thaliana root tip synchronization by hydroxyurea treatment. A major advantage of the method is the possibility of investigating available Arabidopsis cell-cycle mutants without the need to generate cell cultures. As a proof of concept, the effects of over-expression of a dominant negative allele of the B-type cyclin-dependent kinase CDKB1;1 gene on cell-cycle progression were tested. The previously observed prolonged G(2) phase was confirmed, but was found to be compensated for by a reduced G(1) phase. Furthermore, altered S-phase kinetics indicated a functional role for CDKB1;1 during the replication process.},
  author       = {Cools, Toon and Iantcheva, Anelia and Maes, Sara and Van Den Daele, Hilde and De Veylder, Lieven},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {cell cycle,Arabidopsis thaliana,CDKB1,1,hydroxyurea,root meristem,synchronization,CYCLIN-DEPENDENT KINASES,GENE-EXPRESSION,CELL-DIVISION,TRANSCRIPTION FACTOR,PLANT DEVELOPMENT,GENOTOXIC STRESS,DNA-DAMAGE,S-PERIOD,PROGRESSION,COMPLEXES},
  language     = {eng},
  number       = {4},
  pages        = {705--714},
  title        = {A replication stress-induced synchronization method for Arabidopsis thaliana root meristems},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2010.04361.x},
  volume       = {64},
  year         = {2010},
}

Chicago
Cools, Toon, Anelia Iantcheva, Sara Maes, Hilde Van Den Daele, and Lieven De Veylder. 2010. “A Replication Stress-induced Synchronization Method for Arabidopsis Thaliana Root Meristems.” Plant Journal 64 (4): 705–714.
APA
Cools, T., Iantcheva, A., Maes, S., Van Den Daele, H., & De Veylder, L. (2010). A replication stress-induced synchronization method for Arabidopsis thaliana root meristems. PLANT JOURNAL, 64(4), 705–714.
Vancouver
1.
Cools T, Iantcheva A, Maes S, Van Den Daele H, De Veylder L. A replication stress-induced synchronization method for Arabidopsis thaliana root meristems. PLANT JOURNAL. 2010;64(4):705–14.
MLA
Cools, Toon, Anelia Iantcheva, Sara Maes, et al. “A Replication Stress-induced Synchronization Method for Arabidopsis Thaliana Root Meristems.” PLANT JOURNAL 64.4 (2010): 705–714. Print.