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A replication stress-induced synchronization method for Arabidopsis thaliana root meristems

(2010) PLANT JOURNAL. 64(4). p.705-714
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Abstract
Synchronized cell cultures are an indispensable tool for the identification and understanding of key regulators of the cell cycle. Nevertheless, the use of cell cultures has its disadvantages, because it represents an artificial system that does not completely mimic the endogenous conditions that occur in organized meristems. Here, we present a new and easy method for Arabidopsis thaliana root tip synchronization by hydroxyurea treatment. A major advantage of the method is the possibility of investigating available Arabidopsis cell-cycle mutants without the need to generate cell cultures. As a proof of concept, the effects of over-expression of a dominant negative allele of the B-type cyclin-dependent kinase CDKB1;1 gene on cell-cycle progression were tested. The previously observed prolonged G(2) phase was confirmed, but was found to be compensated for by a reduced G(1) phase. Furthermore, altered S-phase kinetics indicated a functional role for CDKB1;1 during the replication process.
Keywords
cell cycle, Arabidopsis thaliana, CDKB1, 1, hydroxyurea, root meristem, synchronization, CYCLIN-DEPENDENT KINASES, GENE-EXPRESSION, CELL-DIVISION, TRANSCRIPTION FACTOR, PLANT DEVELOPMENT, GENOTOXIC STRESS, DNA-DAMAGE, S-PERIOD, PROGRESSION, COMPLEXES

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Citation

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Chicago
Cools, Toon, Anelia Iantcheva, Sara Maes, Hilde Van Den Daele, and Lieven De Veylder. 2010. “A Replication Stress-induced Synchronization Method for Arabidopsis Thaliana Root Meristems.” Plant Journal 64 (4): 705–714.
APA
Cools, T., Iantcheva, A., Maes, S., Van Den Daele, H., & De Veylder, L. (2010). A replication stress-induced synchronization method for Arabidopsis thaliana root meristems. PLANT JOURNAL, 64(4), 705–714.
Vancouver
1.
Cools T, Iantcheva A, Maes S, Van Den Daele H, De Veylder L. A replication stress-induced synchronization method for Arabidopsis thaliana root meristems. PLANT JOURNAL. 2010;64(4):705–14.
MLA
Cools, Toon, Anelia Iantcheva, Sara Maes, et al. “A Replication Stress-induced Synchronization Method for Arabidopsis Thaliana Root Meristems.” PLANT JOURNAL 64.4 (2010): 705–714. Print.
@article{1092961,
  abstract     = {Synchronized cell cultures are an indispensable tool for the identification and understanding of key regulators of the cell cycle. Nevertheless, the use of cell cultures has its disadvantages, because it represents an artificial system that does not completely mimic the endogenous conditions that occur in organized meristems. Here, we present a new and easy method for Arabidopsis thaliana root tip synchronization by hydroxyurea treatment. A major advantage of the method is the possibility of investigating available Arabidopsis cell-cycle mutants without the need to generate cell cultures. As a proof of concept, the effects of over-expression of a dominant negative allele of the B-type cyclin-dependent kinase CDKB1;1 gene on cell-cycle progression were tested. The previously observed prolonged G(2) phase was confirmed, but was found to be compensated for by a reduced G(1) phase. Furthermore, altered S-phase kinetics indicated a functional role for CDKB1;1 during the replication process.},
  author       = {Cools, Toon and Iantcheva, Anelia and Maes, Sara and Van Den Daele, Hilde and De Veylder, Lieven},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {cell cycle,Arabidopsis thaliana,CDKB1,1,hydroxyurea,root meristem,synchronization,CYCLIN-DEPENDENT KINASES,GENE-EXPRESSION,CELL-DIVISION,TRANSCRIPTION FACTOR,PLANT DEVELOPMENT,GENOTOXIC STRESS,DNA-DAMAGE,S-PERIOD,PROGRESSION,COMPLEXES},
  language     = {eng},
  number       = {4},
  pages        = {705--714},
  title        = {A replication stress-induced synchronization method for Arabidopsis thaliana root meristems},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2010.04361.x},
  volume       = {64},
  year         = {2010},
}

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