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The influence of early embryo traits on human embryonic stem cell derivation efficiency

Thomas O'Leary UGent, Björn Heindryckx UGent, Sylvie Lierman UGent, Margot Van der Jeught UGent, Björn Menten UGent, Dieter Deforce UGent, Maria Cornelissen UGent, Susana Chuva de Sousa Lopes and Petra De Sutter UGent (2011) STEM CELLS AND DEVELOPMENT. 20(5). p.785-793
abstract
Despite its prognostic value in in vitro fertilization, early embryo morphology is not reported on in the derivation of human embryonic stem cell (hESC) lines. Standard hESC derivation does rely on blastocyst development and its efficiency is highly correlated to inner cell mass (ICM) quality. Poor-quality embryos (PQEs) donated for hESC derivation may have a range of cleavage-stage abnormalities that are known to compromise further development. This study was implemented to determine whether specific PQEs traits influence the efficiency of good-quality ICMs to derive new hESC lines. We found that although the types of PQEs investigated were all able to make blastocysts with good-quality ICMs, the ICMs were unequal in their ability to derive hESCs. Good-quality ICMs from embryos with multiple poor-quality traits were unable to generate hESC lines, in contrast to good-quality ICMs from embryos with a single poor-quality trait. In addition, our data suggest a direct correlation between the number of ICM cells present in the blastocyst and its capacity to derive new hESC lines. This study is the first to demonstrate that ICM quality alone is an incomplete indicator of hESC derivation and that application of in vitro fertilization-based early embryo scoring can help predict hESC derivation efficiency. Experiments aiming to quantify, improve upon, or compare hESC derivation efficiency should thus take into consideration early embryo morphology scoring for the comparison of groups with equal developmental competence.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PREGNANCY RATES, LINES, DISCARDED EMBRYOS, SUPERNUMERARY EMBRYOS, PREIMPLANTATION DEVELOPMENT, BLASTOCYST DEVELOPMENT, INTRACYTOPLASMIC SPERM INJECTION, POOR-QUALITY EMBRYOS, CLEAVAGE-STAGE EMBRYOS, IN-VITRO FERTILIZATION
journal title
STEM CELLS AND DEVELOPMENT
Stem Cells Dev.
volume
20
issue
5
pages
785 - 793
Web of Science type
Article
Web of Science id
000290255300004
JCR category
TRANSPLANTATION
JCR impact factor
4.459 (2011)
JCR rank
2/24 (2011)
JCR quartile
1 (2011)
ISSN
1547-3287
DOI
10.1089/scd.2010.0338
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1092750
handle
http://hdl.handle.net/1854/LU-1092750
date created
2010-12-23 19:51:18
date last changed
2011-09-27 15:32:15
@article{1092750,
  abstract     = {Despite its prognostic value in in vitro fertilization, early embryo morphology is not reported on in the derivation of human embryonic stem cell (hESC) lines. Standard hESC derivation does rely on blastocyst development and its efficiency is highly correlated to inner cell mass (ICM) quality. Poor-quality embryos (PQEs) donated for hESC derivation may have a range of cleavage-stage abnormalities that are known to compromise further development. This study was implemented to determine whether specific PQEs traits influence the efficiency of good-quality ICMs to derive new hESC lines. We found that although the types of PQEs investigated were all able to make blastocysts with good-quality ICMs, the ICMs were unequal in their ability to derive hESCs. Good-quality ICMs from embryos with multiple poor-quality traits were unable to generate hESC lines, in contrast to good-quality ICMs from embryos with a single poor-quality trait. In addition, our data suggest a direct correlation between the number of ICM cells present in the blastocyst and its capacity to derive new hESC lines. This study is the first to demonstrate that ICM quality alone is an incomplete indicator of hESC derivation and that application of in vitro fertilization-based early embryo scoring can help predict hESC derivation efficiency. Experiments aiming to quantify, improve upon, or compare hESC derivation efficiency should thus take into consideration early embryo morphology scoring for the comparison of groups with equal developmental competence.},
  author       = {O'Leary, Thomas and Heindryckx, Bj{\"o}rn and Lierman, Sylvie and Van der Jeught, Margot and Menten, Bj{\"o}rn and Deforce, Dieter and Cornelissen, Maria and Chuva de Sousa Lopes, Susana and De Sutter, Petra},
  issn         = {1547-3287},
  journal      = {STEM CELLS AND DEVELOPMENT},
  keyword      = {PREGNANCY RATES,LINES,DISCARDED EMBRYOS,SUPERNUMERARY EMBRYOS,PREIMPLANTATION DEVELOPMENT,BLASTOCYST DEVELOPMENT,INTRACYTOPLASMIC SPERM INJECTION,POOR-QUALITY EMBRYOS,CLEAVAGE-STAGE EMBRYOS,IN-VITRO FERTILIZATION},
  language     = {eng},
  number       = {5},
  pages        = {785--793},
  title        = {The influence of early embryo traits on human embryonic stem cell derivation efficiency},
  url          = {http://dx.doi.org/10.1089/scd.2010.0338},
  volume       = {20},
  year         = {2011},
}

Chicago
O’Leary, Thomas, Björn Heindryckx, Sylvie Lierman, Margot Van der Jeught, Björn Menten, Dieter Deforce, Maria Cornelissen, Susana Chuva de Sousa Lopes, and Petra De Sutter. 2011. “The Influence of Early Embryo Traits on Human Embryonic Stem Cell Derivation Efficiency.” Stem Cells and Development 20 (5): 785–793.
APA
O’Leary, T., Heindryckx, B., Lierman, S., Van der Jeught, M., Menten, B., Deforce, D., Cornelissen, M., et al. (2011). The influence of early embryo traits on human embryonic stem cell derivation efficiency. STEM CELLS AND DEVELOPMENT, 20(5), 785–793.
Vancouver
1.
O’Leary T, Heindryckx B, Lierman S, Van der Jeught M, Menten B, Deforce D, et al. The influence of early embryo traits on human embryonic stem cell derivation efficiency. STEM CELLS AND DEVELOPMENT. 2011;20(5):785–93.
MLA
O’Leary, Thomas, Björn Heindryckx, Sylvie Lierman, et al. “The Influence of Early Embryo Traits on Human Embryonic Stem Cell Derivation Efficiency.” STEM CELLS AND DEVELOPMENT 20.5 (2011): 785–793. Print.