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Use of blood culture medium enrichment for synovial fluid culture in horses: a comparison of different culture methods

Michèle Dumoulin UGent, Frederik Pille UGent, A-M van den Abeele, Filip Boyen UGent, B Boussauw, Maarten Oosterlinck UGent, Frank Pasmans UGent, Frank Gasthuys UGent and Ann Martens UGent (2010) EQUINE VETERINARY JOURNAL. 42(6). p.541-546
abstract
Reasons for performing study: Standard methods for culturing equine synovial fluid (SF) are often unrewarding. Evidence-based information on the relative efficiency of different systems used for optimisation of isolation of microorganisms from equine SF is lacking. Objectives: To compare the results of different culture systems performed in parallel on SF samples from horses clinically diagnosed with synovial sepsis. Methods: Synovial fluid specimens were collected between February 2007 and October 2008 from all horses admitted to a referral hospital that were clinically diagnosed with synovial sepsis and from control horses. Synovial fluid samples were cultured in parallel by: 1) direct agar culture (DA); agar culture after: 2) lysis-centrifugation pretreatment (LC); 3) conventional enrichment (CE); 4) combined LC/CE; or 5) blood culture medium enrichment using an automated system (BACTEC 9050). Results: Ninety SF samples from 82 horses were included, together with 40 control samples. Seventy-one of 90 samples (79%) were culture-positive by using blood culture medium enrichment (BACTEC), which was significantly higher compared to all other methods. BACTEC enrichment was never negative while any of the other methods was positive. Although agar culture following LC and/or CE resulted in a slightly higher number of positive samples compared to DA, this difference was not significant. All control samples were culture negative by the 5 different techniques. Although the majority of samples containing isolates recovered without enrichment, culture results after BACTEC enrichment were available on the same day as for agar culture with or without LC (19/23 samples), while CE postponed recovery by at least one day in 20/23 samples. Conclusion: Blood culture medium enrichment is superior to other techniques for isolation of bacteria from SF of horses. The use of an automated system allows enrichment without substantially postponing recovery of microorganisms. Potential relevance: The efficient and fast isolation of microorganisms from infected SF by the BACTEC system allows for rapid susceptibility testing and a more appropriate antibiotic treatment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
bacterial culture, blood culture medium enrichment, synovial infection, horse, BACTEC 9050, AMBULATORY PERITONEAL-DIALYSIS, BACTERIAL ARTHRITIS, PEDIATRIC-PATIENTS, SEPTIC ARTHRITIS, ROUTINE CULTURE, BROTH CULTURES, JOINT FLUID, SYSTEM, BACTEC-9240, SPECIMENS
journal title
EQUINE VETERINARY JOURNAL
Equine Vet. J.
volume
42
issue
6
pages
541 - 546
Web of Science type
Article
Web of Science id
000280997000013
JCR category
VETERINARY SCIENCES
JCR impact factor
1.799 (2010)
JCR rank
20/145 (2010)
JCR quartile
1 (2010)
ISSN
0425-1644
DOI
10.1111/j.2042-3306.2010.00091.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1091158
handle
http://hdl.handle.net/1854/LU-1091158
date created
2010-12-22 13:19:56
date last changed
2010-12-23 09:48:34
@article{1091158,
  abstract     = {Reasons for performing study: Standard methods for culturing equine synovial fluid (SF) are often unrewarding. Evidence-based information on the relative efficiency of different systems used for optimisation of isolation of microorganisms from equine SF is lacking.
Objectives: To compare the results of different culture systems performed in parallel on SF samples from horses clinically diagnosed with synovial sepsis.
Methods: Synovial fluid specimens were collected between February 2007 and October 2008 from all horses admitted to a referral hospital that were clinically diagnosed with synovial sepsis and from control horses. Synovial fluid samples were cultured in parallel by: 1) direct agar culture (DA); agar culture after: 2) lysis-centrifugation pretreatment (LC); 3) conventional enrichment (CE); 4) combined LC/CE; or 5) blood culture medium enrichment using an automated system (BACTEC 9050).
Results: Ninety SF samples from 82 horses were included, together with 40 control samples. Seventy-one of 90 samples (79\%) were culture-positive by using blood culture medium enrichment (BACTEC), which was significantly higher compared to all other methods. BACTEC enrichment was never negative while any of the other methods was positive. Although agar culture following LC and/or CE resulted in a slightly higher number of positive samples compared to DA, this difference was not significant. All control samples were culture negative by the 5 different techniques. Although the majority of samples containing isolates recovered without enrichment, culture results after BACTEC enrichment were available on the same day as for agar culture with or without LC (19/23 samples), while CE postponed recovery by at least one day in 20/23 samples.
Conclusion: Blood culture medium enrichment is superior to other techniques for isolation of bacteria from SF of horses. The use of an automated system allows enrichment without substantially postponing recovery of microorganisms.
Potential relevance: The efficient and fast isolation of microorganisms from infected SF by the BACTEC system allows for rapid susceptibility testing and a more appropriate antibiotic treatment.},
  author       = {Dumoulin, Mich{\`e}le and Pille, Frederik and van den Abeele, A-M and Boyen, Filip and Boussauw, B and Oosterlinck, Maarten and Pasmans, Frank and Gasthuys, Frank and Martens, Ann},
  issn         = {0425-1644},
  journal      = {EQUINE VETERINARY JOURNAL},
  keyword      = {bacterial culture,blood culture medium enrichment,synovial infection,horse,BACTEC 9050,AMBULATORY PERITONEAL-DIALYSIS,BACTERIAL ARTHRITIS,PEDIATRIC-PATIENTS,SEPTIC ARTHRITIS,ROUTINE CULTURE,BROTH CULTURES,JOINT FLUID,SYSTEM,BACTEC-9240,SPECIMENS},
  language     = {eng},
  number       = {6},
  pages        = {541--546},
  title        = {Use of blood culture medium enrichment for synovial fluid culture in horses: a comparison of different culture methods},
  url          = {http://dx.doi.org/10.1111/j.2042-3306.2010.00091.x},
  volume       = {42},
  year         = {2010},
}

Chicago
Dumoulin, Michèle, Frederik Pille, A-M van den Abeele, Filip Boyen, B Boussauw, Maarten Oosterlinck, Frank Pasmans, Frank Gasthuys, and Ann Martens. 2010. “Use of Blood Culture Medium Enrichment for Synovial Fluid Culture in Horses: a Comparison of Different Culture Methods.” Equine Veterinary Journal 42 (6): 541–546.
APA
Dumoulin, M., Pille, F., van den Abeele, A.-M., Boyen, F., Boussauw, B., Oosterlinck, M., Pasmans, F., et al. (2010). Use of blood culture medium enrichment for synovial fluid culture in horses: a comparison of different culture methods. EQUINE VETERINARY JOURNAL, 42(6), 541–546.
Vancouver
1.
Dumoulin M, Pille F, van den Abeele A-M, Boyen F, Boussauw B, Oosterlinck M, et al. Use of blood culture medium enrichment for synovial fluid culture in horses: a comparison of different culture methods. EQUINE VETERINARY JOURNAL. 2010;42(6):541–6.
MLA
Dumoulin, Michèle, Frederik Pille, A-M van den Abeele, et al. “Use of Blood Culture Medium Enrichment for Synovial Fluid Culture in Horses: a Comparison of Different Culture Methods.” EQUINE VETERINARY JOURNAL 42.6 (2010): 541–546. Print.