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Lack of a correlation between γH2AX foci kinetics in lymphocytes and the severity of acute normal tissue reactions during IMRT treatment for head and neck cancer

Joke Werbrouck (UGent) , Fréderic Duprez (UGent) , Wilfried De Neve (UGent) and Hubert Thierens (UGent)
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Abstract
Purpose: To investigate the phosphorylated histone H2A isoform X (γH2AX) foci kinetics as an indicator for the development of acute normal tissue complications during Intensity-Modulated Radiotherapy (IMRT) for head and neck cancer (HNC) patients. Materials and methods: Microscopic scoring of the gH2AX foci was used to evaluate the DNA-double-strand-break repair capacity in from Ataxia-Telangiectasia (A-T) patients derived lymphoblastoid cell lines (LCL) and T-lymphocytes isolated from 31 IMRT treated HNC patients. Cells were irradiated in vitro with 0.5 Gy given at high-dose-rate (HDR) and examined at several times up to 24 h after irradiation. The patients were subdivided in three groups showing mild, moderate and severe acute normal tissue complications based on their Common Toxicity Criteria grades for dysphagia, mucositis and dermatitis during the radiotherapy course. Results: For the ATM (Ataxia-Telangiectasia-Mutated) defective LCL, a lower number of radiation-induced foci and a somewhat less efficient repair capacity was observed. No correlation was found between the gH2AX foci kinetics pattern and the risk for acute normal tissue complications among the three patient subgroups. Conclusions: Scoring of gH2AX foci after in vitro irradiation of isolated T-lymphocytes of HNC patients cannot be applied to predict for the development of acute normal tissue complications.
Keywords
γH2AX foci, radiosensitivity assay, acute normal tissue complications, DNA DSB repair, head and neck cancer

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Chicago
Werbrouck, Joke, Fréderic Duprez, Wilfried De Neve, and Hubert Thierens. 2011. “Lack of a Correlation Between γH2AX Foci Kinetics in Lymphocytes and the Severity of Acute Normal Tissue Reactions During IMRT Treatment for Head and Neck Cancer.” International Journal of Radiation Biology 87 (1): 46–56.
APA
Werbrouck, Joke, Duprez, F., De Neve, W., & Thierens, H. (2011). Lack of a correlation between γH2AX foci kinetics in lymphocytes and the severity of acute normal tissue reactions during IMRT treatment for head and neck cancer. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 87(1), 46–56.
Vancouver
1.
Werbrouck J, Duprez F, De Neve W, Thierens H. Lack of a correlation between γH2AX foci kinetics in lymphocytes and the severity of acute normal tissue reactions during IMRT treatment for head and neck cancer. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2011;87(1):46–56.
MLA
Werbrouck, Joke, Fréderic Duprez, Wilfried De Neve, et al. “Lack of a Correlation Between γH2AX Foci Kinetics in Lymphocytes and the Severity of Acute Normal Tissue Reactions During IMRT Treatment for Head and Neck Cancer.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 87.1 (2011): 46–56. Print.
@article{1090388,
  abstract     = {Purpose: To investigate the phosphorylated histone H2A isoform X (\ensuremath{\gamma}H2AX) foci kinetics as an indicator for the development of acute normal tissue complications during Intensity-Modulated Radiotherapy (IMRT) for head and neck cancer (HNC) patients.
Materials and methods: Microscopic scoring of the gH2AX foci was used to evaluate the DNA-double-strand-break repair capacity in from Ataxia-Telangiectasia (A-T) patients derived lymphoblastoid cell lines (LCL) and T-lymphocytes isolated from 31 IMRT treated HNC patients. Cells were irradiated in vitro with 0.5 Gy given at high-dose-rate (HDR) and examined at several times up to 24 h after irradiation. The patients were subdivided in three groups showing mild, moderate and severe acute normal tissue complications based on their Common Toxicity Criteria grades for dysphagia, mucositis and dermatitis during the radiotherapy course.
Results: For the ATM (Ataxia-Telangiectasia-Mutated) defective LCL, a lower number of radiation-induced foci and a somewhat less efficient repair capacity was observed. No correlation was found between the gH2AX foci kinetics pattern and the risk for acute normal tissue complications among the three patient subgroups.
Conclusions: Scoring of gH2AX foci after in vitro irradiation of isolated T-lymphocytes of HNC patients cannot be applied to predict for the development of acute normal tissue complications.},
  author       = {Werbrouck, Joke and Duprez, Fr{\'e}deric and De Neve, Wilfried and Thierens, Hubert},
  issn         = {1362-3095},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {\ensuremath{\gamma}H2AX foci,radiosensitivity assay,acute normal tissue complications,DNA DSB repair,head and neck cancer},
  language     = {eng},
  number       = {1},
  pages        = {46--56},
  title        = {Lack of a correlation between \ensuremath{\gamma}H2AX foci kinetics in lymphocytes and the severity of acute normal tissue reactions during IMRT treatment for head and neck cancer},
  url          = {http://dx.doi.org/10.3109/09553002.2010.518213},
  volume       = {87},
  year         = {2011},
}

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