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Two novel proteins expressed by the venom glands of Apis mellifera and Nasonia vitripennis share an ancient C1q-like domain

Dirk de Graaf UGent, Marleen Brunain UGent, B Scharlaken, N Peiren, Bart Devreese UGent, DG Ebo, WJ Stevens, CA Desjardins, JH Werren and Franciscus Jacobs UGent (2010) INSECT MOLECULAR BIOLOGY. 19(suppl. 1). p.1-10
abstract
An in-depth proteomic study of previously unidentified two-dimensional polyacrylamide gel electrophoresis spots of honey bee (Apis mellifera, Hymenoptera) venom revealed a new protein with a C1q conserved domain (C1q-VP). BLASTP searching revealed a strong identity with only two proteins from other insect species: the jewel wasp, Nasonia vitripennis (Hymenoptera), and the green pea aphid, Acyrthosiphon pisum (Hemiptera). In higher organisms, C1q is the first subcomponent of the classical complement pathway and constitutes a major link between innate and acquired immunity. Expression of C1q-VP in a variety of tissues of honey bee workers and drones was demonstrated. In addition, a wide spatial and temporal pattern of expression was observed in N. vitripennis. We suggest that C1q-VP represents a new member of the emerging group of venom trace elements. Using degenerate primers the corresponding gene was found to be highly conserved in eight hymenopteran species, including species of the Aculeata and the Parasitica groups (suborder Apocrita) and even the suborder Symphyta. A preliminary test using recombinant proteins failed to demonstrate Am_C1q-VP-specific immunoglobulin E recognition by serum from patients with a documented severe bee venom allergy.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Nasonia vitripennis, venom, insect, Hymenoptera, Apis mellifera, HONEYBEE VENOM, STRUCTURAL-CHARACTERIZATION, GLOBULAR DOMAIN, HOST, WASP, BEE, HYMENOPTERA, METABOLISM, FLY
journal title
INSECT MOLECULAR BIOLOGY
Insect Mol. Biol.
volume
19
issue
suppl. 1
pages
1 - 10
Web of Science type
Article
Web of Science id
000273947400003
JCR category
ENTOMOLOGY
JCR impact factor
2.669 (2010)
JCR rank
5/83 (2010)
JCR quartile
1 (2010)
ISSN
0962-1075
DOI
10.1111/j.1365-2583.2009.00913.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1089753
handle
http://hdl.handle.net/1854/LU-1089753
date created
2010-12-20 13:05:06
date last changed
2010-12-20 16:03:44
@article{1089753,
  abstract     = {An in-depth proteomic study of previously unidentified two-dimensional polyacrylamide gel electrophoresis spots of honey bee (Apis mellifera, Hymenoptera) venom revealed a new protein with a C1q conserved domain (C1q-VP). BLASTP searching revealed a strong identity with only two proteins from other insect species: the jewel wasp, Nasonia vitripennis (Hymenoptera), and the green pea aphid, Acyrthosiphon pisum (Hemiptera). In higher organisms, C1q is the first subcomponent of the classical complement pathway and constitutes a major link between innate and acquired immunity. Expression of C1q-VP in a variety of tissues of honey bee workers and drones was demonstrated. In addition, a wide spatial and temporal pattern of expression was observed in N. vitripennis. We suggest that C1q-VP represents a new member of the emerging group of venom trace elements. Using degenerate primers the corresponding gene was found to be highly conserved in eight hymenopteran species, including species of the Aculeata and the Parasitica groups (suborder Apocrita) and even the suborder Symphyta. A preliminary test using recombinant proteins failed to demonstrate Am\_C1q-VP-specific immunoglobulin E recognition by serum from patients with a documented severe bee venom allergy.},
  author       = {de Graaf, Dirk and Brunain, Marleen and Scharlaken, B and Peiren, N and Devreese, Bart and Ebo, DG and Stevens, WJ and Desjardins, CA and Werren, JH and Jacobs, Franciscus},
  issn         = {0962-1075},
  journal      = {INSECT MOLECULAR BIOLOGY},
  keyword      = {Nasonia vitripennis,venom,insect,Hymenoptera,Apis mellifera,HONEYBEE VENOM,STRUCTURAL-CHARACTERIZATION,GLOBULAR DOMAIN,HOST,WASP,BEE,HYMENOPTERA,METABOLISM,FLY},
  language     = {eng},
  number       = {suppl. 1},
  pages        = {1--10},
  title        = {Two novel proteins expressed by the venom glands of Apis mellifera and Nasonia vitripennis share an ancient C1q-like domain},
  url          = {http://dx.doi.org/10.1111/j.1365-2583.2009.00913.x},
  volume       = {19},
  year         = {2010},
}

Chicago
de Graaf, Dirk, Marleen Brunain, B Scharlaken, N Peiren, Bart Devreese, DG Ebo, WJ Stevens, CA Desjardins, JH Werren, and Franciscus Jacobs. 2010. “Two Novel Proteins Expressed by the Venom Glands of Apis Mellifera and Nasonia Vitripennis Share an Ancient C1q-like Domain.” Insect Molecular Biology 19 (suppl. 1): 1–10.
APA
de Graaf, Dirk, Brunain, M., Scharlaken, B., Peiren, N., Devreese, B., Ebo, D., Stevens, W., et al. (2010). Two novel proteins expressed by the venom glands of Apis mellifera and Nasonia vitripennis share an ancient C1q-like domain. INSECT MOLECULAR BIOLOGY, 19(suppl. 1), 1–10.
Vancouver
1.
de Graaf D, Brunain M, Scharlaken B, Peiren N, Devreese B, Ebo D, et al. Two novel proteins expressed by the venom glands of Apis mellifera and Nasonia vitripennis share an ancient C1q-like domain. INSECT MOLECULAR BIOLOGY. 2010;19(suppl. 1):1–10.
MLA
de Graaf, Dirk, Marleen Brunain, B Scharlaken, et al. “Two Novel Proteins Expressed by the Venom Glands of Apis Mellifera and Nasonia Vitripennis Share an Ancient C1q-like Domain.” INSECT MOLECULAR BIOLOGY 19.suppl. 1 (2010): 1–10. Print.