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Disposition of hop prenylflavonoids in human breast tissue

(2010) MOLECULAR NUTRITION & FOOD RESEARCH. 54(Suppl. 2). p.S284-S294
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Abstract
Hop-derived products may contain xanthohumol (XN), isoxanthohumol (IX), and the potent phytoestrogen 8-prenylnaringenin (8-PN). To evaluate the potential health effects of these prenylflavonoids on breast tissue, their concentration, nature of metabolites, and biodistribution were assessed and compared with 17 beta-estradiol (E(2)) exposure. In this dietary intervention study, women were randomly allocated to hop (n = 11; 2.04 mg XN, 1.20 mg IX, and 0.1 mg 8-PN per supplement) or control (n = 10). After a run-in of >= 4 days, three supplements were taken daily for 5 days preceding an aesthetic breast reduction. Blood and breast biopsies were analyzed using HPLC-ESI-MS/MS. Upon hop administration, XN and IX concentrations ranged between 0.72 and 17.65 nmol/L and 3.30 and 31.50 nmol/L, and between 0.26 and 5.14 pmol/g and 1.16 and 83.67 pmol/g in hydrolyzed serum and breast tissue, respectively. 8-PN however, was only detected in samples of moderate and strong 8-PN producers (0.43-7.06 nmol/L and 0.78-4.83 pmol/g). Phase I metabolism appeared to be minor (similar to 10%), whereas extensive glucuronidation was observed (> 90%). Total prenylflavonoids showed a breast adipose/glandular tissue distribution of 38/62 and their derived E(2)-equivalents were negligible compared with E(2) in adipose (384.6 +/- 118.8 fmol/g, p = 0.009) and glandular (241.6 +/- 93.1 fmol/g, p<0.001) tissue, respectively. Consequently, low doses of prenylflavonoids are unlikely to elicit estrogenic responses in breast tissue.
Keywords
HUMAN LIVER-MICROSOMES, HUMULUS-LUPULUS L., ESTROGEN-RECEPTOR-ALPHA, IN-VITRO, POTENT PHYTOESTROGEN, POSTMENOPAUSAL WOMEN, LIQUID-CHROMATOGRAPHY, AROMATASE ACTIVITIES, ISOFLAVONE METABOLITES, MASS-SPECTROMETRY, 8-prenylnaringenin, 17 beta-estradiol, Bioavailability, Breast tissue, Humulus lupulus

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MLA
Bolca, Selin, Jinghu Li, Dejan Nikolic, et al. “Disposition of Hop Prenylflavonoids in Human Breast Tissue.” MOLECULAR NUTRITION & FOOD RESEARCH 54.Suppl. 2 (2010): S284–S294. Print.
APA
Bolca, S., Li, J., Nikolic, D., Roche, N., Blondeel, P., Possemiers, S., De Keukeleire, D., et al. (2010). Disposition of hop prenylflavonoids in human breast tissue. MOLECULAR NUTRITION & FOOD RESEARCH, 54(Suppl. 2), S284–S294.
Chicago author-date
Bolca, Selin, Jinghu Li, Dejan Nikolic, Nathalie Roche, Phillip Blondeel, Sam Possemiers, Denis De Keukeleire, et al. 2010. “Disposition of Hop Prenylflavonoids in Human Breast Tissue.” Molecular Nutrition & Food Research 54 (Suppl. 2): S284–S294.
Chicago author-date (all authors)
Bolca, Selin, Jinghu Li, Dejan Nikolic, Nathalie Roche, Phillip Blondeel, Sam Possemiers, Denis De Keukeleire, Marc Bracke, Arne Heyerick, Richard B Van Breemen, and Herman Depypere. 2010. “Disposition of Hop Prenylflavonoids in Human Breast Tissue.” Molecular Nutrition & Food Research 54 (Suppl. 2): S284–S294.
Vancouver
1.
Bolca S, Li J, Nikolic D, Roche N, Blondeel P, Possemiers S, et al. Disposition of hop prenylflavonoids in human breast tissue. MOLECULAR NUTRITION & FOOD RESEARCH. 2010;54(Suppl. 2):S284–S294.
IEEE
[1]
S. Bolca et al., “Disposition of hop prenylflavonoids in human breast tissue,” MOLECULAR NUTRITION & FOOD RESEARCH, vol. 54, no. Suppl. 2, pp. S284–S294, 2010.
@article{1089245,
  abstract     = {Hop-derived products may contain xanthohumol (XN), isoxanthohumol (IX), and the potent phytoestrogen 8-prenylnaringenin (8-PN). To evaluate the potential health effects of these prenylflavonoids on breast tissue, their concentration, nature of metabolites, and biodistribution were assessed and compared with 17 beta-estradiol (E(2)) exposure. In this dietary intervention study, women were randomly allocated to hop (n = 11; 2.04 mg XN, 1.20 mg IX, and 0.1 mg 8-PN per supplement) or control (n = 10). After a run-in of >= 4 days, three supplements were taken daily for 5 days preceding an aesthetic breast reduction. Blood and breast biopsies were analyzed using HPLC-ESI-MS/MS. Upon hop administration, XN and IX concentrations ranged between 0.72 and 17.65 nmol/L and 3.30 and 31.50 nmol/L, and between 0.26 and 5.14 pmol/g and 1.16 and 83.67 pmol/g in hydrolyzed serum and breast tissue, respectively. 8-PN however, was only detected in samples of moderate and strong 8-PN producers (0.43-7.06 nmol/L and 0.78-4.83 pmol/g). Phase I metabolism appeared to be minor (similar to 10%), whereas extensive glucuronidation was observed (> 90%). Total prenylflavonoids showed a breast adipose/glandular tissue distribution of 38/62 and their derived E(2)-equivalents were negligible compared with E(2) in adipose (384.6 +/- 118.8 fmol/g, p = 0.009) and glandular (241.6 +/- 93.1 fmol/g, p<0.001) tissue, respectively. Consequently, low doses of prenylflavonoids are unlikely to elicit estrogenic responses in breast tissue.},
  author       = {Bolca, Selin and Li, Jinghu and Nikolic, Dejan and Roche, Nathalie and Blondeel, Phillip and Possemiers, Sam and De Keukeleire, Denis and Bracke, Marc and Heyerick, Arne and Van Breemen, Richard B and Depypere, Herman},
  issn         = {1613-4125},
  journal      = {MOLECULAR NUTRITION & FOOD RESEARCH},
  keywords     = {HUMAN LIVER-MICROSOMES,HUMULUS-LUPULUS L.,ESTROGEN-RECEPTOR-ALPHA,IN-VITRO,POTENT PHYTOESTROGEN,POSTMENOPAUSAL WOMEN,LIQUID-CHROMATOGRAPHY,AROMATASE ACTIVITIES,ISOFLAVONE METABOLITES,MASS-SPECTROMETRY,8-prenylnaringenin,17 beta-estradiol,Bioavailability,Breast tissue,Humulus lupulus},
  language     = {eng},
  number       = {Suppl. 2},
  pages        = {S284--S294},
  title        = {Disposition of hop prenylflavonoids in human breast tissue},
  url          = {http://dx.doi.org/10.1002/mnfr.200900519},
  volume       = {54},
  year         = {2010},
}

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