Ghent University Academic Bibliography

Advanced

Disposition of hop prenylflavonoids in human breast tissue

Selin Bolca UGent, Jinghu Li, Dejan Nikolic, NATHALIE ROCHE UGent, Phillip Blondeel UGent, Sam Possemiers UGent, Denis De Keukeleire UGent, Marc Bracke UGent, Arne Heyerick UGent and Richard B Van Breemen, et al. (2010) MOLECULAR NUTRITION & FOOD RESEARCH. 54(Suppl. 2). p.S284-S294
abstract
Hop-derived products may contain xanthohumol (XN), isoxanthohumol (IX), and the potent phytoestrogen 8-prenylnaringenin (8-PN). To evaluate the potential health effects of these prenylflavonoids on breast tissue, their concentration, nature of metabolites, and biodistribution were assessed and compared with 17 beta-estradiol (E(2)) exposure. In this dietary intervention study, women were randomly allocated to hop (n = 11; 2.04 mg XN, 1.20 mg IX, and 0.1 mg 8-PN per supplement) or control (n = 10). After a run-in of >= 4 days, three supplements were taken daily for 5 days preceding an aesthetic breast reduction. Blood and breast biopsies were analyzed using HPLC-ESI-MS/MS. Upon hop administration, XN and IX concentrations ranged between 0.72 and 17.65 nmol/L and 3.30 and 31.50 nmol/L, and between 0.26 and 5.14 pmol/g and 1.16 and 83.67 pmol/g in hydrolyzed serum and breast tissue, respectively. 8-PN however, was only detected in samples of moderate and strong 8-PN producers (0.43-7.06 nmol/L and 0.78-4.83 pmol/g). Phase I metabolism appeared to be minor (similar to 10%), whereas extensive glucuronidation was observed (> 90%). Total prenylflavonoids showed a breast adipose/glandular tissue distribution of 38/62 and their derived E(2)-equivalents were negligible compared with E(2) in adipose (384.6 +/- 118.8 fmol/g, p = 0.009) and glandular (241.6 +/- 93.1 fmol/g, p<0.001) tissue, respectively. Consequently, low doses of prenylflavonoids are unlikely to elicit estrogenic responses in breast tissue.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HUMAN LIVER-MICROSOMES, HUMULUS-LUPULUS L., ESTROGEN-RECEPTOR-ALPHA, IN-VITRO, POTENT PHYTOESTROGEN, POSTMENOPAUSAL WOMEN, LIQUID-CHROMATOGRAPHY, AROMATASE ACTIVITIES, ISOFLAVONE METABOLITES, MASS-SPECTROMETRY, 8-prenylnaringenin, 17 beta-estradiol, Bioavailability, Breast tissue, Humulus lupulus
journal title
MOLECULAR NUTRITION & FOOD RESEARCH
Mol. Nutr. Food Res.
volume
54
issue
Suppl. 2
pages
S284 - S294
Web of Science type
Article
Web of Science id
000294244400018
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
4.713 (2010)
JCR rank
1/124 (2010)
JCR quartile
1 (2010)
ISSN
1613-4125
DOI
10.1002/mnfr.200900519
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1089245
handle
http://hdl.handle.net/1854/LU-1089245
date created
2010-12-17 15:57:18
date last changed
2011-11-23 10:38:29
@article{1089245,
  abstract     = {Hop-derived products may contain xanthohumol (XN), isoxanthohumol (IX), and the potent phytoestrogen 8-prenylnaringenin (8-PN). To evaluate the potential health effects of these prenylflavonoids on breast tissue, their concentration, nature of metabolites, and biodistribution were assessed and compared with 17 beta-estradiol (E(2)) exposure. In this dietary intervention study, women were randomly allocated to hop (n = 11; 2.04 mg XN, 1.20 mg IX, and 0.1 mg 8-PN per supplement) or control (n = 10). After a run-in of {\textrangle}= 4 days, three supplements were taken daily for 5 days preceding an aesthetic breast reduction. Blood and breast biopsies were analyzed using HPLC-ESI-MS/MS. Upon hop administration, XN and IX concentrations ranged between 0.72 and 17.65 nmol/L and 3.30 and 31.50 nmol/L, and between 0.26 and 5.14 pmol/g and 1.16 and 83.67 pmol/g in hydrolyzed serum and breast tissue, respectively. 8-PN however, was only detected in samples of moderate and strong 8-PN producers (0.43-7.06 nmol/L and 0.78-4.83 pmol/g). Phase I metabolism appeared to be minor (similar to 10\%), whereas extensive glucuronidation was observed ({\textrangle} 90\%). Total prenylflavonoids showed a breast adipose/glandular tissue distribution of 38/62 and their derived E(2)-equivalents were negligible compared with E(2) in adipose (384.6 +/- 118.8 fmol/g, p = 0.009) and glandular (241.6 +/- 93.1 fmol/g, p{\textlangle}0.001) tissue, respectively. Consequently, low doses of prenylflavonoids are unlikely to elicit estrogenic responses in breast tissue.},
  author       = {Bolca, Selin and Li, Jinghu and Nikolic, Dejan and ROCHE, NATHALIE and Blondeel, Phillip and Possemiers, Sam and De Keukeleire, Denis and Bracke, Marc and Heyerick, Arne and Van Breemen, Richard B and Depypere, Herman},
  issn         = {1613-4125},
  journal      = {MOLECULAR NUTRITION \& FOOD RESEARCH},
  keyword      = {HUMAN LIVER-MICROSOMES,HUMULUS-LUPULUS L.,ESTROGEN-RECEPTOR-ALPHA,IN-VITRO,POTENT PHYTOESTROGEN,POSTMENOPAUSAL WOMEN,LIQUID-CHROMATOGRAPHY,AROMATASE ACTIVITIES,ISOFLAVONE METABOLITES,MASS-SPECTROMETRY,8-prenylnaringenin,17 beta-estradiol,Bioavailability,Breast tissue,Humulus lupulus},
  language     = {eng},
  number       = {Suppl. 2},
  pages        = {S284--S294},
  title        = {Disposition of hop prenylflavonoids in human breast tissue},
  url          = {http://dx.doi.org/10.1002/mnfr.200900519},
  volume       = {54},
  year         = {2010},
}

Chicago
Bolca, Selin, Jinghu Li, Dejan Nikolic, NATHALIE ROCHE, Phillip Blondeel, Sam Possemiers, Denis De Keukeleire, et al. 2010. “Disposition of Hop Prenylflavonoids in Human Breast Tissue.” Molecular Nutrition & Food Research 54 (Suppl. 2): S284–S294.
APA
Bolca, S., Li, J., Nikolic, D., ROCHE, N., Blondeel, P., Possemiers, S., De Keukeleire, D., et al. (2010). Disposition of hop prenylflavonoids in human breast tissue. MOLECULAR NUTRITION & FOOD RESEARCH, 54(Suppl. 2), S284–S294.
Vancouver
1.
Bolca S, Li J, Nikolic D, ROCHE N, Blondeel P, Possemiers S, et al. Disposition of hop prenylflavonoids in human breast tissue. MOLECULAR NUTRITION & FOOD RESEARCH. 2010;54(Suppl. 2):S284–S294.
MLA
Bolca, Selin, Jinghu Li, Dejan Nikolic, et al. “Disposition of Hop Prenylflavonoids in Human Breast Tissue.” MOLECULAR NUTRITION & FOOD RESEARCH 54.Suppl. 2 (2010): S284–S294. Print.