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Lipid mobilization in subcutaneous adipose tissue during exercise in lean and obese humans: roles of insulin and natriuretic peptides

Katrien Koppo UGent, Dominique Larrouy, Marie Marques, Michel Berlan, Magda Bajzova, Jan Polak, Johan Van de Voorde UGent, Jens Bülow, Max Lafontan and François Crampes, et al. (2010) AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 299(2). p.E258-E265
abstract
The aim of this study was to evaluate the relative contributions of various hormones involved in the regulation of lipid mobilization in subcutaneous adipose tissue (SCAT) during exercise and to assess the impact of obesity on this regulation. Eight lean and eight obese men performed a 60-min cycle exercise bout at 50% of their peak oxygen uptake on two occasions: during intravenous infusion of octreotide (a somatostatin analog) or physiological saline (control condition). Lipolysis in SCAT was evaluated using in situ microdialysis. One microdialysis probe was perfused with the adrenergic blockers phentolamine and propranolol while another probe was perfused with the phosphodiesterase and adenosine receptor inhibitor aminophylline. Compared with the control condition, infusion of octreotide reduced plasma insulin levels in lean (from similar to 3.5 to 0.5 mu U/ml) and in obese (from similar to 9 to 2 mu U/ml), blunted the exercise-induced rise in plasma GH and epinephrine levels in both groups, and enhanced the exercise-induced natriuretic peptide (NP) levels in lean but not in obese subjects. In both groups, octreotide infusion resulted in higher exercise-induced increases in dialysate glycerol concentrations in the phentolamine-containing probe while no difference in lipolytic response was found in the aminophylline-containing probe. The results suggest that insulin antilipolytic action plays a role in the regulation of lipolysis during exercise in lean as well as in obese subjects. The octreotide-induced enhancement of exercise lipolysis in lean subjects was associated with an increased exercise-induced plasma NP response. Adenosine may contribute to the inhibition of basal lipolysis in both subject groups.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HUMAN ADIPOCYTES, ADRENERGIC REGULATION, SOMATOSTATIN ANALOG, OCTREOTIDE, CORTISOL, PATHWAY, SENSITIVITY, METABOLISM, LIPOLYSIS, GROWTH-HORMONE, octreotide adenosine catecholamines microdialysis exercise-induced liplysis
journal title
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Am. J. Physiol.-Endocrinol. Metab.
volume
299
issue
2
pages
E258 - E265
Web of Science type
Article
Web of Science id
000279654600014
JCR category
PHYSIOLOGY
JCR impact factor
4.686 (2010)
JCR rank
9/77 (2010)
JCR quartile
1 (2010)
ISSN
0193-1849
DOI
10.1152/ajpendo.00767.2009
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1089128
handle
http://hdl.handle.net/1854/LU-1089128
date created
2010-12-17 14:19:52
date last changed
2010-12-20 10:10:41
@article{1089128,
  abstract     = {The aim of this study was to evaluate the relative contributions of various hormones involved in the regulation of lipid mobilization in subcutaneous adipose tissue (SCAT) during exercise and to assess the impact of obesity on this regulation. Eight lean and eight obese men performed a 60-min cycle exercise bout at 50\% of their peak oxygen uptake on two occasions: during intravenous infusion of octreotide (a somatostatin analog) or physiological saline (control condition). Lipolysis in SCAT was evaluated using in situ microdialysis. One microdialysis probe was perfused with the adrenergic blockers phentolamine and propranolol while another probe was perfused with the phosphodiesterase and adenosine receptor inhibitor aminophylline. Compared with the control condition, infusion of octreotide reduced plasma insulin levels in lean (from similar to 3.5 to 0.5 mu U/ml) and in obese (from similar to 9 to 2 mu U/ml), blunted the exercise-induced rise in plasma GH and epinephrine levels in both groups, and enhanced the exercise-induced natriuretic peptide (NP) levels in lean but not in obese subjects. In both groups, octreotide infusion resulted in higher exercise-induced increases in dialysate glycerol concentrations in the phentolamine-containing probe while no difference in lipolytic response was found in the aminophylline-containing probe. The results suggest that insulin antilipolytic action plays a role in the regulation of lipolysis during exercise in lean as well as in obese subjects. The octreotide-induced enhancement of exercise lipolysis in lean subjects was associated with an increased exercise-induced plasma NP response. Adenosine may contribute to the inhibition of basal lipolysis in both subject groups.},
  author       = {Koppo, Katrien and Larrouy, Dominique and Marques, Marie and Berlan, Michel and Bajzova, Magda and Polak, Jan and Van de Voorde, Johan and B{\"u}low, Jens and Lafontan, Max and Crampes, Fran\c{c}ois and Langin, Dominique and Stich, Vladimir  and de Glisezinski, Isabelle },
  issn         = {0193-1849},
  journal      = {AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM},
  keyword      = {HUMAN ADIPOCYTES,ADRENERGIC REGULATION,SOMATOSTATIN ANALOG,OCTREOTIDE,CORTISOL,PATHWAY,SENSITIVITY,METABOLISM,LIPOLYSIS,GROWTH-HORMONE,octreotide adenosine catecholamines microdialysis exercise-induced liplysis},
  language     = {eng},
  number       = {2},
  pages        = {E258--E265},
  title        = {Lipid mobilization in subcutaneous adipose tissue during exercise in lean and obese humans: roles of insulin and natriuretic peptides},
  url          = {http://dx.doi.org/10.1152/ajpendo.00767.2009},
  volume       = {299},
  year         = {2010},
}

Chicago
Koppo, Katrien, Dominique Larrouy, Marie Marques, Michel Berlan, Magda Bajzova, Jan Polak, Johan Van de Voorde, et al. 2010. “Lipid Mobilization in Subcutaneous Adipose Tissue During Exercise in Lean and Obese Humans: Roles of Insulin and Natriuretic Peptides.” American Journal of Physiology-endocrinology and Metabolism 299 (2): E258–E265.
APA
Koppo, K., Larrouy, D., Marques, M., Berlan, M., Bajzova, M., Polak, J., Van de Voorde, J., et al. (2010). Lipid mobilization in subcutaneous adipose tissue during exercise in lean and obese humans: roles of insulin and natriuretic peptides. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 299(2), E258–E265.
Vancouver
1.
Koppo K, Larrouy D, Marques M, Berlan M, Bajzova M, Polak J, et al. Lipid mobilization in subcutaneous adipose tissue during exercise in lean and obese humans: roles of insulin and natriuretic peptides. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 2010;299(2):E258–E265.
MLA
Koppo, Katrien, Dominique Larrouy, Marie Marques, et al. “Lipid Mobilization in Subcutaneous Adipose Tissue During Exercise in Lean and Obese Humans: Roles of Insulin and Natriuretic Peptides.” AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 299.2 (2010): E258–E265. Print.