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Plasmacytoid dendritic cells in pulmonary lymphoid follicles of patients with COPD

Geert Van Pottelberge, Ken Bracke UGent, Sarah Van den Broeck, Suzanne M Reinartz, Cornelis M van Drunen, Emiel FM Wouters, Geert M Verleden, Frank Vermassen UGent, Guy Joos UGent and Guy Brusselle UGent (2010) EUROPEAN RESPIRATORY JOURNAL. 36(4). p.781-791
abstract
Plasmacytoid dendritic cells (pDCs) are professional antigen-presenting cells with antiviral and tolerogenic capabilities. Viral infections and autoimmunity are proposed to be important mechanisms in the pathogenesis of chronic obstructive pulmonary disease (COPD). The study aimed to quantify blood dendritic cell antigen 2-positive pDCs in lungs of subjects with or without COPD by immunohistochemistry and flow cytometry, combined with the investigation of the influence of cigarette smoke extract (CSE) on the function of pDCs in vitro. pDCs were mainly located in lymphoid follicles, a finding compatible with their expression of lymphoid homing chemokine receptors CXCR3 and CXCR4. pDC accumulated in the lymphoid follicles and in lung digests of patients with mild to moderate COPD, compared with smokers without airflow limitation and patients with COPD Global Initiative for Chronic Obstructive Lung disease (GOLD) stage III-IV. Exposing maturing pDC of healthy subjects to CSE in vitro revealed an attenuation of the expression of co-stimulatory molecules and impaired interferon-a production. Maturing pDC from patients with COPD produced higher levels of tumour necrosis factor (TNF)-alpha and interleukin (IL)-8 compared to pDC from healthy subjects. CSE significantly impairs the antiviral function of pDCs. In COPD, a GOLD stage dependent accumulation of pDC in lymphoid follicles is present, combined with an enhanced production of TNF-alpha and IL-8 by maturing pDCs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
T-REGULATORY-CELLS, HUMAN LUNG, BRONCHOALVEOLAR LAVAGE FLUID, plasmacytoid dendritic cells, lymphoid follicle, dendritic cell maturation, cigarette smoke, chronic obstructive pulmonary disease, Airway inflammation, INFLAMMATORY DISEASES, SMALL-AIRWAY, PATHOGENESIS, SUPPRESSES, MECHANISMS, INFECTION, SUBSETS
journal title
EUROPEAN RESPIRATORY JOURNAL
Eur. Resp. J.
volume
36
issue
4
pages
781 - 791
Web of Science type
Article
Web of Science id
000282473700018
JCR category
RESPIRATORY SYSTEM
JCR impact factor
5.922 (2010)
JCR rank
4/45 (2010)
JCR quartile
1 (2010)
ISSN
0903-1936
DOI
10.1183/09031936.00140409
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1087866
handle
http://hdl.handle.net/1854/LU-1087866
date created
2010-12-15 15:07:17
date last changed
2016-12-19 15:46:18
@article{1087866,
  abstract     = {Plasmacytoid dendritic cells (pDCs) are professional antigen-presenting cells with antiviral and tolerogenic capabilities. Viral infections and autoimmunity are proposed to be important mechanisms in the pathogenesis of chronic obstructive pulmonary disease (COPD). 
The study aimed to quantify blood dendritic cell antigen 2-positive pDCs in lungs of subjects with or without COPD by immunohistochemistry and flow cytometry, combined with the investigation of the influence of cigarette smoke extract (CSE) on the function of pDCs in vitro.
pDCs were mainly located in lymphoid follicles, a finding compatible with their expression of lymphoid homing chemokine receptors CXCR3 and CXCR4. pDC accumulated in the lymphoid follicles and in lung digests of patients with mild to moderate COPD, compared with smokers without airflow limitation and patients with COPD Global Initiative for Chronic Obstructive Lung disease (GOLD) stage III-IV. Exposing maturing pDC of healthy subjects to CSE in vitro revealed an attenuation of the expression of co-stimulatory molecules and impaired interferon-a production. Maturing pDC from patients with COPD produced higher levels of tumour necrosis factor (TNF)-alpha and interleukin (IL)-8 compared to pDC from healthy subjects.
CSE significantly impairs the antiviral function of pDCs. In COPD, a GOLD stage dependent accumulation of pDC in lymphoid follicles is present, combined with an enhanced production of TNF-alpha and IL-8 by maturing pDCs.},
  author       = {Van Pottelberge, Geert and Bracke, Ken and Van den Broeck, Sarah and Reinartz, Suzanne M and van Drunen, Cornelis M and Wouters, Emiel FM and Verleden, Geert M and Vermassen, Frank and Joos, Guy and Brusselle, Guy},
  issn         = {0903-1936},
  journal      = {EUROPEAN RESPIRATORY JOURNAL},
  keyword      = {T-REGULATORY-CELLS,HUMAN LUNG,BRONCHOALVEOLAR LAVAGE FLUID,plasmacytoid dendritic cells,lymphoid follicle,dendritic cell maturation,cigarette smoke,chronic obstructive pulmonary disease,Airway inflammation,INFLAMMATORY DISEASES,SMALL-AIRWAY,PATHOGENESIS,SUPPRESSES,MECHANISMS,INFECTION,SUBSETS},
  language     = {eng},
  number       = {4},
  pages        = {781--791},
  title        = {Plasmacytoid dendritic cells in pulmonary lymphoid follicles of patients with COPD},
  url          = {http://dx.doi.org/10.1183/09031936.00140409},
  volume       = {36},
  year         = {2010},
}

Chicago
Van Pottelberge, Geert, Ken Bracke, Sarah Van den Broeck, Suzanne M Reinartz, Cornelis M van Drunen, Emiel FM Wouters, Geert M Verleden, Frank Vermassen, Guy Joos, and Guy Brusselle. 2010. “Plasmacytoid Dendritic Cells in Pulmonary Lymphoid Follicles of Patients with COPD.” European Respiratory Journal 36 (4): 781–791.
APA
Van Pottelberge, G., Bracke, K., Van den Broeck, S., Reinartz, S. M., van Drunen, C. M., Wouters, E. F., Verleden, G. M., et al. (2010). Plasmacytoid dendritic cells in pulmonary lymphoid follicles of patients with COPD. EUROPEAN RESPIRATORY JOURNAL, 36(4), 781–791.
Vancouver
1.
Van Pottelberge G, Bracke K, Van den Broeck S, Reinartz SM, van Drunen CM, Wouters EF, et al. Plasmacytoid dendritic cells in pulmonary lymphoid follicles of patients with COPD. EUROPEAN RESPIRATORY JOURNAL. 2010;36(4):781–91.
MLA
Van Pottelberge, Geert, Ken Bracke, Sarah Van den Broeck, et al. “Plasmacytoid Dendritic Cells in Pulmonary Lymphoid Follicles of Patients with COPD.” EUROPEAN RESPIRATORY JOURNAL 36.4 (2010): 781–791. Print.